Q9Z1J2 · NEK4_MOUSE
- ProteinSerine/threonine-protein kinase Nek4
- GeneNek4
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids792 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Required for normal entry into proliferative arrest after a limited number of cell divisions, also called replicative senescence. Required for normal cell cycle arrest in response to double-stranded DNA damage (By similarity).
Protein kinase that seems to act exclusively upon threonine residues
Protein kinase that seems to act exclusively upon threonine residues
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]
Cofactor
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | ciliary basal body | |
Cellular Component | ciliary plasm | |
Cellular Component | ciliary rootlet | |
Cellular Component | ciliary transition zone | |
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | manganese ion binding | |
Molecular Function | protein serine kinase activity | |
Molecular Function | protein serine/threonine kinase activity | |
Biological Process | cell division | |
Biological Process | DNA damage response | |
Biological Process | mitotic cell cycle | |
Biological Process | protein phosphorylation | |
Biological Process | regulation of cellular senescence |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSerine/threonine-protein kinase Nek4
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ9Z1J2
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000086426 | 1-792 | Serine/threonine-protein kinase Nek4 | |||
Sequence: MPQAAYCYMRVVGRGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYIKHHISLFLEATKAKTSKNNVKNCDSRAKPVAAVVSRKEESNTDVIHYQPRSSEGSALHVMGEDKCLSQEKPVDIGPLRSPASLEGHTGKQDMNNTGESCATISRINIDILPAERRDSANAGVVQESQPQHVDAADEVDSQCSISQEKERLQGNTKSSDQPGNLLPRRSSDGGDGEGSELVKPLYPSNKDQKPDQDQVTGIIENQDSIHPRSQPHSSMSEPSLSRQRRQKKREQTAHSGTKSQFQELPPRLLPSYPGIGKVDIIATQQNDGNQGGPVAGCVNSSRTSSTASAKDRPLSARERRRLKQSQEEMLPSGPAVQRTPSAVEPLKPQEEDQPIPAQRFSSDCSITQMNHTLPREKEKRLMHGLSEDELSSSTSSTDKSDGDSREGKSHTNEMKDLVQLMTQTLRLEAKESCEDLQVLNPGSEFRLHRKYRDTLVLHGKVAEEVEPHCTELPTGIIPGSEKIRRIVEVLRADVIQGLGIQLLEQVFDLLGEEDELEREARLQEHMGDKYTTYCVKARQLKFFEENVSF | ||||||
Modified residue | 165 | Phosphothreonine; by autocatalysis | ||||
Sequence: T | ||||||
Modified residue | 340 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 343 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 566 | N6-methyllysine | ||||
Sequence: K | ||||||
Modified residue | 675 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed ubiquitously among various organs and is up-regulated in the testis.
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 6-261 | Protein kinase | ||||
Sequence: YCYMRVVGRGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYI | ||||||
Region | 329-358 | Disordered | ||||
Sequence: QEKPVDIGPLRSPASLEGHTGKQDMNNTGE | ||||||
Region | 379-515 | Disordered | ||||
Sequence: ANAGVVQESQPQHVDAADEVDSQCSISQEKERLQGNTKSSDQPGNLLPRRSSDGGDGEGSELVKPLYPSNKDQKPDQDQVTGIIENQDSIHPRSQPHSSMSEPSLSRQRRQKKREQTAHSGTKSQFQELPPRLLPSY | ||||||
Compositional bias | 399-425 | Polar residues | ||||
Sequence: DSQCSISQEKERLQGNTKSSDQPGNLL | ||||||
Compositional bias | 450-483 | Polar residues | ||||
Sequence: DQKPDQDQVTGIIENQDSIHPRSQPHSSMSEPSL | ||||||
Compositional bias | 527-554 | Polar residues | ||||
Sequence: QQNDGNQGGPVAGCVNSSRTSSTASAKD | ||||||
Region | 527-611 | Disordered | ||||
Sequence: QQNDGNQGGPVAGCVNSSRTSSTASAKDRPLSARERRRLKQSQEEMLPSGPAVQRTPSAVEPLKPQEEDQPIPAQRFSSDCSITQ | ||||||
Compositional bias | 555-569 | Basic and acidic residues | ||||
Sequence: RPLSARERRRLKQSQ | ||||||
Region | 628-657 | Disordered | ||||
Sequence: LSEDELSSSTSSTDKSDGDSREGKSHTNEM | ||||||
Compositional bias | 642-656 | Basic and acidic residues | ||||
Sequence: KSDGDSREGKSHTNE |
Sequence similarities
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q9Z1J2-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- SynonymsmSTK2L
- Length792
- Mass (Da)88,994
- Last updated1999-05-01 v1
- ChecksumCF9187311C807A1D
Q9Z1J2-2
- Name2
- SynonymsmSTK2S
- Differences from canonical
- 456-503: Missing
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q3THY0 | Q3THY0_MOUSE | Nek4 | 274 | ||
A0A2I3BPM0 | A0A2I3BPM0_MOUSE | Nek4 | 630 | ||
F8WGA9 | F8WGA9_MOUSE | Nek4 | 737 | ||
Q6GTE9 | Q6GTE9_MOUSE | Nek4 | 792 |
Features
Showing features for compositional bias, alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 399-425 | Polar residues | ||||
Sequence: DSQCSISQEKERLQGNTKSSDQPGNLL | ||||||
Compositional bias | 450-483 | Polar residues | ||||
Sequence: DQKPDQDQVTGIIENQDSIHPRSQPHSSMSEPSL | ||||||
Alternative sequence | VSP_007001 | 456-503 | in isoform 2 | |||
Sequence: Missing | ||||||
Sequence conflict | 499 | in Ref. 2; AAD16287 | ||||
Sequence: G → R | ||||||
Compositional bias | 527-554 | Polar residues | ||||
Sequence: QQNDGNQGGPVAGCVNSSRTSSTASAKD | ||||||
Compositional bias | 555-569 | Basic and acidic residues | ||||
Sequence: RPLSARERRRLKQSQ | ||||||
Compositional bias | 642-656 | Basic and acidic residues | ||||
Sequence: KSDGDSREGKSHTNE |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ223071 EMBL· GenBank· DDBJ | CAA11072.1 EMBL· GenBank· DDBJ | mRNA | ||
Y09234 EMBL· GenBank· DDBJ | CAA70436.1 EMBL· GenBank· DDBJ | mRNA | ||
AF099067 EMBL· GenBank· DDBJ | AAD16287.1 EMBL· GenBank· DDBJ | mRNA |