Q9Z191 · EYA4_MOUSE
- ProteinEyes absent homolog 4
- GeneEya4
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids616 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye (By similarity).
Catalytic activity
- H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Features
Showing features for active site, binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | metal ion binding | |
Molecular Function | protein tyrosine phosphatase activity | |
Biological Process | cell differentiation | |
Biological Process | chromatin organization | |
Biological Process | DNA repair | |
Biological Process | middle ear morphogenesis | |
Biological Process | negative regulation of extrinsic apoptotic signaling pathway in absence of ligand | |
Biological Process | positive regulation of DNA repair | |
Biological Process | sensory perception of sound |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEyes absent homolog 4
- EC number
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ9Z191
- Secondary accessions
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for modified residue, chain, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 1 | N-acetylmethionine | ||||
Sequence: M | ||||||
Chain | PRO_0000218652 | 1-616 | Eyes absent homolog 4 | |||
Sequence: MEDTQDLNEQSVKKTCPEADVSEPQNSRSMEMQDLASPHALVGGSDTPGSSKLDKSGLSSTSVTTNGTGVSLLAVKTEPLHSSESTTTTGDGALDTFTGSVITSSGYSPRSAQQYSPQLYPSKPYPHILSTPAAQTMSAYAGQTQYSGMQQPAVYTAYSQTGQPYSLPAYDLGVMLPAIKTESGLSQTQSPLQSGCLSYSPGFSTPQPGQTPYSYQMPGSSFAPSSTIYANNSVSNSTNFSSSQQDYPSYTAFGQNQYAQYYSASTYGAYMTSNNTADGTSSSTSTYQLQESLQGLTSQPGEFDTVQSPSTPIKDLDDRTCRSSGSKSRGRGRKNNPSPPPDSDLERVFVWDLDETIIVFHSLLTGSYAQKYGKDPPMAVTLGLRMEEMIFNLADTHLFFNDLEECDQVHIDDVSSDDNGQDLSTYSFATDGFHAAASSANLCLPTGVRGGVDWMRKLAFRYRRVKELYNTYKNNVGGLLGPAKRDAWLQLRAEIEGLTDSWLTNALKSLSIISTRSNCVNVLVTTTQLIPALAKVLLYSLGGAFPIENIYSATKIGKESCFERIVSRFGTNITYVVIGDGRDEEHAANQHNMPFWRISSHSDLLALHQALELEYL | ||||||
Cross-link | 14 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 52 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Modified residue | 338 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
In the embryo, expressed mainly in the craniofacial mesenchyme, dermamyotome and limb.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-66 | Disordered | ||||
Sequence: MEDTQDLNEQSVKKTCPEADVSEPQNSRSMEMQDLASPHALVGGSDTPGSSKLDKSGLSSTSVTTN | ||||||
Compositional bias | 49-66 | Polar residues | ||||
Sequence: GSSKLDKSGLSSTSVTTN | ||||||
Region | 186-211 | Disordered | ||||
Sequence: SQTQSPLQSGCLSYSPGFSTPQPGQT | ||||||
Compositional bias | 277-314 | Polar residues | ||||
Sequence: ADGTSSSTSTYQLQESLQGLTSQPGEFDTVQSPSTPIK | ||||||
Region | 277-345 | Disordered | ||||
Sequence: ADGTSSSTSTYQLQESLQGLTSQPGEFDTVQSPSTPIKDLDDRTCRSSGSKSRGRGRKNNPSPPPDSDL |
Sequence similarities
Belongs to the HAD-like hydrolase superfamily. EYA family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length616
- Mass (Da)66,875
- Last updated2011-07-27 v2
- ChecksumAC4B7E891AB7A6F3
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1W2P721 | A0A1W2P721_MOUSE | Eya4 | 639 | ||
A0A1W2P897 | A0A1W2P897_MOUSE | Eya4 | 74 | ||
Q8BY78 | Q8BY78_MOUSE | Eya4 | 616 |
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 49-66 | Polar residues | ||||
Sequence: GSSKLDKSGLSSTSVTTN | ||||||
Compositional bias | 277-314 | Polar residues | ||||
Sequence: ADGTSSSTSTYQLQESLQGLTSQPGEFDTVQSPSTPIK | ||||||
Sequence conflict | 337 | in Ref. 1; CAA76637 | ||||
Sequence: P → L |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Y17115 EMBL· GenBank· DDBJ | CAA76637.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ007997 EMBL· GenBank· DDBJ | CAA07820.1 EMBL· GenBank· DDBJ | mRNA | ||
CH466540 EMBL· GenBank· DDBJ | EDL04766.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC120899 EMBL· GenBank· DDBJ | AAI20900.1 EMBL· GenBank· DDBJ | mRNA |