Q9Y2A9 · B3GN3_HUMAN
- ProteinN-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3
- GeneB3GNT3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids372 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides (PubMed:11042166).
Also acts as a core1-1,3-N-acetylglucosaminyltransferase (Core1-beta3GlcNAcT) to form the 6-sulfo sialyl Lewis x on extended core1 O-glycans (PubMed:11439191).
Also acts as a core1-1,3-N-acetylglucosaminyltransferase (Core1-beta3GlcNAcT) to form the 6-sulfo sialyl Lewis x on extended core1 O-glycans (PubMed:11439191).
Catalytic activity
- 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-{beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->3)-[beta-D-GlcNAc-(1->6)]-alpha-D-GalNAc}-L-threonyl-[protein] + H+ + UDP
- 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-{beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->3)-[beta-D-GlcNAc-(1->6)]-alpha-D-GalNAc}-L-seryl-[protein] + H+ + UDP
Pathway
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Golgi membrane | |
Cellular Component | plasma membrane | |
Molecular Function | beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity | |
Molecular Function | N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity | |
Molecular Function | UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity | |
Biological Process | keratan sulfate biosynthetic process | |
Biological Process | O-glycan processing | |
Biological Process | poly-N-acetyllactosamine biosynthetic process | |
Biological Process | protein O-linked glycosylation |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameN-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ9Y2A9
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-10 | Cytoplasmic | ||||
Sequence: MKYLRHRRPN | ||||||
Transmembrane | 11-31 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: ATLILAIGAFTLLLFSLLVSP | ||||||
Topological domain | 32-372 | Lumenal | ||||
Sequence: PTCKVQEQPPAIPEALAWPTPPTRPAPAPCHANTSMVTHPDFATQPQHVQNFLLYRHCRHFPLLQDVPPSKCAQPVFLLLVIKSSPSNYVRRELLRRTWGRERKVRGLQLRLLFLVGTASNPHEARKVNRLLELEAQTHGDILQWDFHDSFFNLTLKQVLFLQWQETRCANASFVLNGDDDVFAHTDNMVFYLQDHDPGRHLFVGQLIQNVGPIRAFWSKYYVPEVVTQNERYPPYCGGGGFLLSRFTAAALRRAAHVLDIFPIDDVFLGMCLELEGLKPASHSGIRTSGVRAPSQRLSSFDPCFYRDLLLVHRFLPYEMLLMWDALNQPNLTCGNQTQIY |
Keywords
- Cellular component
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_074178 | 317 | ||||
Sequence: I → F | ||||||
Natural variant | VAR_022644 | 328 | in dbSNP:rs36686 | |||
Sequence: R → H |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 501 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000219172 | 1-372 | N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3 | |||
Sequence: MKYLRHRRPNATLILAIGAFTLLLFSLLVSPPTCKVQEQPPAIPEALAWPTPPTRPAPAPCHANTSMVTHPDFATQPQHVQNFLLYRHCRHFPLLQDVPPSKCAQPVFLLLVIKSSPSNYVRRELLRRTWGRERKVRGLQLRLLFLVGTASNPHEARKVNRLLELEAQTHGDILQWDFHDSFFNLTLKQVLFLQWQETRCANASFVLNGDDDVFAHTDNMVFYLQDHDPGRHLFVGQLIQNVGPIRAFWSKYYVPEVVTQNERYPPYCGGGGFLLSRFTAAALRRAAHVLDIFPIDDVFLGMCLELEGLKPASHSGIRTSGVRAPSQRLSSFDPCFYRDLLLVHRFLPYEMLLMWDALNQPNLTCGNQTQIY | ||||||
Glycosylation | 64 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 184 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 202 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 362 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 367 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in colon, jejunum, stomach, esophagus, placenta and trachea.
Gene expression databases
Organism-specific databases
Structure
Sequence
- Sequence statusComplete
- Length372
- Mass (Da)42,534
- Last updated2010-11-02 v2
- ChecksumA6E3FE88B2F00F10
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 89 | in Ref. 9; AAH66876 | ||||
Sequence: C → Y | ||||||
Sequence conflict | 355 | in Ref. 9; AAH67423 | ||||
Sequence: W → R |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB015630 EMBL· GenBank· DDBJ | BAA76497.1 EMBL· GenBank· DDBJ | mRNA | ||
AF293973 EMBL· GenBank· DDBJ | AAK00849.1 EMBL· GenBank· DDBJ | mRNA | ||
AB049585 EMBL· GenBank· DDBJ | BAB21531.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ130847 EMBL· GenBank· DDBJ | CAC45044.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ278961 EMBL· GenBank· DDBJ | CAC82374.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AY358955 EMBL· GenBank· DDBJ | AAQ89314.1 EMBL· GenBank· DDBJ | mRNA | ||
AK314323 EMBL· GenBank· DDBJ | BAG36971.1 EMBL· GenBank· DDBJ | mRNA | ||
AC008761 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC066876 EMBL· GenBank· DDBJ | AAH66876.1 EMBL· GenBank· DDBJ | mRNA | ||
BC067423 EMBL· GenBank· DDBJ | AAH67423.1 EMBL· GenBank· DDBJ | mRNA |