Q9Y169 · EXT2_DROME
- ProteinExostosin-2
- Genesotv
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids717 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains. Plays a central role in diffusion of morphogens hedgehog (hh), wingless (wg) and Decapentaplegic (dpp) via its role in heparan sulfate proteoglycans (HSPGs) biosynthesis, HSPGs being required for movement of Hh, Dpp and wg morphogens.
Catalytic activity
- 3-O-{[(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNAc](n)-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl}-L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-{alpha-D-GlcNAc-[(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNAc](n)-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl}-L-seryl-[protein] + H+ + UDP
3-O-{[(1→4)-β-D-GlcA-(1→4)-α-D-GlcNAc](n)-(1→4)-β-D-GlcA-(1→3)-β-D-Gal-(1→3)-β-D-Gal-(1→4)-β-D-Xyl}-L-seryl-[protein] RHEA-COMP:12621 + CHEBI:57705 = 3-O-{α-D-GlcNAc-[(1→4)-β-D-GlcA-(1→4)-α-D-GlcNAc](n)-(1→4)-β-D-GlcA-(1→3)-β-D-Gal-(1→3)-β-D-Gal-(1→4)-β-D-Xyl}-L-seryl-[protein] RHEA-COMP:12623 + CHEBI:15378 + CHEBI:58223 - 3-O-{alpha-D-GlcNAc-[(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNAc](n)-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl}-L-seryl-[protein] + UDP-alpha-D-glucuronate = 3-O-{[(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNAc](n+1)-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl}-L-seryl-[protein] + H+ + UDP
Cofactor
Pathway
Protein modification; protein glycosylation.
Glycan metabolism; heparan sulfate biosynthesis.
Glycan metabolism; heparin biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 464 | UDP-N-acetyl-alpha-D-glucosamine (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 489 | UDP-N-acetyl-alpha-D-glucosamine (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 516 | UDP-N-acetyl-alpha-D-glucosamine (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 521 | UDP-N-acetyl-alpha-D-glucosamine (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 537 | UDP-N-acetyl-alpha-D-glucosamine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 538 | UDP-N-acetyl-alpha-D-glucosamine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 539 | Mn2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 539 | UDP-N-acetyl-alpha-D-glucosamine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 626 | UDP-N-acetyl-alpha-D-glucosamine (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 627 | |||||
Sequence: D | ||||||
Binding site | 627 | UDP-N-acetyl-alpha-D-glucosamine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 672 | UDP-N-acetyl-alpha-D-glucosamine (UniProtKB | ChEBI) | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | EXT1-EXT2 complex | |
Cellular Component | Golgi apparatus | |
Cellular Component | Golgi membrane | |
Molecular Function | acetylglucosaminyltransferase activity | |
Molecular Function | glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity | |
Molecular Function | metal ion binding | |
Molecular Function | N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity | |
Biological Process | chondroitin sulfate biosynthetic process | |
Biological Process | glycosaminoglycan biosynthetic process | |
Biological Process | heparan sulfate proteoglycan biosynthetic process | |
Biological Process | heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process | |
Biological Process | heparin biosynthetic process | |
Biological Process | N-acetylglucosamine metabolic process | |
Biological Process | protein glycosylation | |
Biological Process | Wnt signaling pathway |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameExostosin-2
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ9Y169
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass type II membrane protein
Golgi apparatus membrane ; Single-pass type II membrane protein
Note: Localization to the Golgi may be regulated by sau (PubMed:23720043).
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-25 | Cytoplasmic | ||||
Sequence: MTKIKNLLSFVTQSRAISHTNPREH | ||||||
Transmembrane | 26-46 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: ILNCLTYGLLVIVALCAGFLL | ||||||
Topological domain | 47-717 | Lumenal | ||||
Sequence: WDLSSSPRDGFFHGKRDSHTLILDLEHIQELAVNPEAEQRARNVNCTFWDCLNIYKCEHDRLKVYIYPLQEFVDEQSDKTATTLSSEYFQILEAVLKSRYYTSNPNEACLFLPSLDLLNQNVFDKHLAGAALASLDFWDRGANHIIFNMLPGGAPSYNTVLDVNTDNAIIFGGGFDSWSYRPGFDVAIPVWSPRLVRQHAHATAQRKFLLVVAQLNILPRFVRTLRELSLAHSEQLLLLGACENLDLTMRCPLSQHHKSLEYPRLLSRGKFCLLGRSLRMGQPDLVEIMSQHCIPVIAVDNYVLPFEDVIDWSLASVRIRENELHSVMQKLKAISSVKIVEMQKQVQWLFSKYFKDLKTVTLTALEVLESRIFPLRARSSRQWNTIDTNARSTFNPLFLPSLAPKSQGFTAVILTYDRVESLFLLIQKLAVVPSLQSILVIWNNQKKSPPHLSTFPSISKPLKIRQTKENKLSNRFYPYPEIETEAILTIDDDIIMLTTDELDFGYEVWREFPDHIVGFPSRIHVWENVTMRWHYESEWTNQISMVLTGAAFHHKYWSHMYTHAMPGDIKDWVDEHMNCEDIAMNFLVANITNNPPIKVTPRKKFKCPECTNTEMLSADLNHMRERSACIDRFSKIYGRMPLRTVEFRADPVLFRDNFPDKLKRYNDIGSL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000149665 | 1-717 | Exostosin-2 | |||
Sequence: MTKIKNLLSFVTQSRAISHTNPREHILNCLTYGLLVIVALCAGFLLWDLSSSPRDGFFHGKRDSHTLILDLEHIQELAVNPEAEQRARNVNCTFWDCLNIYKCEHDRLKVYIYPLQEFVDEQSDKTATTLSSEYFQILEAVLKSRYYTSNPNEACLFLPSLDLLNQNVFDKHLAGAALASLDFWDRGANHIIFNMLPGGAPSYNTVLDVNTDNAIIFGGGFDSWSYRPGFDVAIPVWSPRLVRQHAHATAQRKFLLVVAQLNILPRFVRTLRELSLAHSEQLLLLGACENLDLTMRCPLSQHHKSLEYPRLLSRGKFCLLGRSLRMGQPDLVEIMSQHCIPVIAVDNYVLPFEDVIDWSLASVRIRENELHSVMQKLKAISSVKIVEMQKQVQWLFSKYFKDLKTVTLTALEVLESRIFPLRARSSRQWNTIDTNARSTFNPLFLPSLAPKSQGFTAVILTYDRVESLFLLIQKLAVVPSLQSILVIWNNQKKSPPHLSTFPSISKPLKIRQTKENKLSNRFYPYPEIETEAILTIDDDIIMLTTDELDFGYEVWREFPDHIVGFPSRIHVWENVTMRWHYESEWTNQISMVLTGAAFHHKYWSHMYTHAMPGDIKDWVDEHMNCEDIAMNFLVANITNNPPIKVTPRKKFKCPECTNTEMLSADLNHMRERSACIDRFSKIYGRMPLRTVEFRADPVLFRDNFPDKLKRYNDIGSL | ||||||
Glycosylation | 91 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 574 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 625↔675 | |||||
Sequence: CEDIAMNFLVANITNNPPIKVTPRKKFKCPECTNTEMLSADLNHMRERSAC | ||||||
Glycosylation | 636 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
In wing imaginal disk, it is ubiquitously expressed.
Developmental stage
Expressed both maternally and zygotically.
Gene expression databases
Interaction
Subunit
Interacts with sau.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9Y169 | ttv Q9V730 | 3 | EBI-142791, EBI-166374 |
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length717
- Mass (Da)82,726
- Last updated1999-11-01 v1
- ChecksumA3894493C16EF7DC
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE013599 EMBL· GenBank· DDBJ | AAO41379.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF145598 EMBL· GenBank· DDBJ | AAD38573.1 EMBL· GenBank· DDBJ | mRNA | ||
BT003544 EMBL· GenBank· DDBJ | AAO39548.1 EMBL· GenBank· DDBJ | mRNA |