Q9XWN7 · NSH1_CAEEL
- ProteinNucleoside hydrolase
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids338 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the hydrolysis of the N-glycosidic bond of commonly occurring purine and pyrimidine nucleosides into ribose and the base, with decreasing activity, in the order: adenosine, guanosine, inosine, xanthosine, cytidine, uridine.
Catalytic activity
- a purine D-ribonucleoside + H2O = a purine nucleobase + D-ribose
- guanosine + H2O = D-ribose + guanine
- xanthosine + H2O = D-ribose + xanthine
Cofactor
Note: Binds 1 Ca2+ ion per monomer.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.295 mM | inosine | 7.0 | 37 | |||
0.129 mM | inosine | 7.0 | 25 | |||
0.093 mM | adenosine | 7.0 | 37 | |||
0.077 mM | adenosine | 7.0 | 25 | |||
0.164 mM | guanosine | 7.0 | 37 | |||
0.068 mM | guanosine | 7.0 | 25 | |||
0.037 mM | 7-methylguanosine | 7.0 | 25 | |||
10.004 mM | cytidine | 7.0 | 37 | |||
2.843 mM | uridine | 7.0 | 37 | |||
1.038 mM | xanthosine | 7.0 | 37 |
kcat is 0.79 sec-1 with inosine as substrate (at pH 7.0 and 37 degrees Celsius) (PubMed:12777783).
kcat is 0.203 sec-1 with inosine as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:28218438).
kcat is 0.78 sec-1 with adenosine as substrate (at pH 7.0 and 37 degrees Celsius) (PubMed:12777783).
kcat is 0.418 sec-1 with adenosine as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:28218438).
kcat is 0.84 sec-1 with guanosine as substrate (at pH 7.0 and 37 degrees Celsius) (PubMed:12777783).
kcat is 0.143 sec-1 with guanosine as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:28218438).
kcat is 0.50 sec-1 with cytidine as substrate (at pH 7.0 and 37 degrees Celsius) (PubMed:12777783).
kcat is 0.002 sec-1 with uridine as substrate (at pH 7.0 and 37 degrees Celsius) (PubMed:12777783).
kcat is 0.029 sec-1 with xanthosine as substrate (at pH 7.0 and 37 degrees Celsius) (PubMed:12777783).
kcat is 0.203 sec-1 with inosine as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:28218438).
kcat is 0.78 sec-1 with adenosine as substrate (at pH 7.0 and 37 degrees Celsius) (PubMed:12777783).
kcat is 0.418 sec-1 with adenosine as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:28218438).
kcat is 0.84 sec-1 with guanosine as substrate (at pH 7.0 and 37 degrees Celsius) (PubMed:12777783).
kcat is 0.143 sec-1 with guanosine as substrate (at pH 7.0 and 25 degrees Celsius) (PubMed:28218438).
kcat is 0.50 sec-1 with cytidine as substrate (at pH 7.0 and 37 degrees Celsius) (PubMed:12777783).
kcat is 0.002 sec-1 with uridine as substrate (at pH 7.0 and 37 degrees Celsius) (PubMed:12777783).
kcat is 0.029 sec-1 with xanthosine as substrate (at pH 7.0 and 37 degrees Celsius) (PubMed:12777783).
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 13 | Proton acceptor | |||
Binding site | 13 | Ca2+ (UniProtKB | ChEBI); catalytic | |||
Binding site | 18 | Ca2+ (UniProtKB | ChEBI); catalytic | |||
Binding site | 133 | Ca2+ (UniProtKB | ChEBI); catalytic | |||
Binding site | 254 | Ca2+ (UniProtKB | ChEBI); catalytic | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | adenosine nucleosidase activity | |
Molecular Function | calcium ion binding | |
Molecular Function | inosine nucleosidase activity | |
Molecular Function | purine nucleosidase activity | |
Biological Process | purine nucleoside catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNucleoside hydrolase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ9XWN7
Proteomes
Organism-specific databases
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 42 | Reduces hydrolysis of inosine, adenosine, and guanosine by a factor of about 14-, 22-, and 10-fold, respectively. | |||
Mutagenesis | 253 | Reduces hydrolysis of inosine, adenosine and guanosine by a factor of about 120-fold. May be involved in leaving group activation, acting as a general acid required to protonate the N7 of the purine leaving group. | |||
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000457212 | 1-338 | Nucleoside hydrolase | ||
Proteomic databases
Expression
Gene expression databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length338
- Mass (Da)37,215
- Last updated1999-11-01 v1
- Checksum2F604EA869EBD250
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX284605 EMBL· GenBank· DDBJ | CAA21614.1 EMBL· GenBank· DDBJ | Genomic DNA |