Q9XT96 · PSN2_BOVIN
- ProteinPresenilin-2
- GenePSEN2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids449 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins. The holoprotein functions as a calcium-leak channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is involved in calcium homeostasis. Is a regulator of mitochondrion-endoplasmic reticulum membrane tethering and modulates calcium ions shuttling between ER and mitochondria.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 264 | |||||
Sequence: D | ||||||
Active site | 367 | |||||
Sequence: D |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | centrosome | |
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | gamma-secretase complex | |
Cellular Component | Golgi membrane | |
Cellular Component | kinetochore | |
Cellular Component | nuclear inner membrane | |
Molecular Function | aspartic endopeptidase activity, intramembrane cleaving | |
Biological Process | amyloid-beta formation | |
Biological Process | calcium ion homeostasis | |
Biological Process | intracellular signal transduction | |
Biological Process | membrane protein ectodomain proteolysis | |
Biological Process | mitochondrion-endoplasmic reticulum membrane tethering | |
Biological Process | Notch signaling pathway | |
Biological Process | protein processing | |
Biological Process | regulation of calcium import into the mitochondrion |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended namePresenilin-2
- EC number
- Short namesPS-2
- Cleaved into 2 chains
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos
Accessions
- Primary accessionQ9XT96
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Golgi apparatus membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane, intramembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-88 | Cytoplasmic | ||||
Sequence: MLTFMASDSEEEVCDERTSLMSAESPTPRSCQDGRQGLEDGESAAQWRSQESEEDHEEEDPDRYVCSGVPGRPPGLEEELTLKYGAKH | ||||||
Transmembrane | 89-109 | Helical | ||||
Sequence: VIMLFVPVTLCMIVVVATIKS | ||||||
Topological domain | 110-139 | Lumenal | ||||
Sequence: VRFYTEKNGQLIYTPFSEDTPSVGQRLLNS | ||||||
Transmembrane | 140-160 | Helical | ||||
Sequence: VLNTLIMISVIVTMTIFLVVL | ||||||
Topological domain | 161-167 | Cytoplasmic | ||||
Sequence: YKYRCYK | ||||||
Transmembrane | 168-188 | Helical | ||||
Sequence: FIHGWLIMSSLMLLFLFTYIY | ||||||
Topological domain | 189-201 | Lumenal | ||||
Sequence: LGEVLKTYNVAMD | ||||||
Transmembrane | 202-222 | Helical | ||||
Sequence: YPTLFLTVWNFGAVGMVCIHW | ||||||
Topological domain | 223-224 | Cytoplasmic | ||||
Sequence: KG | ||||||
Transmembrane | 225-245 | Helical | ||||
Sequence: PLVLQQAYLIMISALMALVFI | ||||||
Topological domain | 246-250 | Lumenal | ||||
Sequence: KYLPE | ||||||
Transmembrane | 251-271 | Helical | ||||
Sequence: WSAWVILGAISVYDLVAVLCP | ||||||
Topological domain | 272-362 | Cytoplasmic | ||||
Sequence: KGPLRMLVETAQERNEPIFPALIYSSAMVWTVGMAKLDPSSQGALQLPYDPEMEEDSYDSFGEPSYPDVFEPPLPGYPGEELEEEEERGVK | ||||||
Transmembrane | 363-383 | Helical | ||||
Sequence: LGLGDFIFYSVLVGKAAATGS | ||||||
Topological domain | 384-389 | Lumenal | ||||
Sequence: GDWNTT | ||||||
Transmembrane | 390-410 | Helical | ||||
Sequence: LACFVAILIGLCLTLLLLAVF | ||||||
Topological domain | 411-414 | Cytoplasmic | ||||
Sequence: KKAL | ||||||
Intramembrane | 415-435 | Helical | ||||
Sequence: PALPISITFGLIFYFSTDNLV | ||||||
Topological domain | 436-449 | Cytoplasmic | ||||
Sequence: RPFMDTLASHQLYI |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000025601 | 1-298 | Presenilin-2 NTF subunit | |||
Sequence: MLTFMASDSEEEVCDERTSLMSAESPTPRSCQDGRQGLEDGESAAQWRSQESEEDHEEEDPDRYVCSGVPGRPPGLEEELTLKYGAKHVIMLFVPVTLCMIVVVATIKSVRFYTEKNGQLIYTPFSEDTPSVGQRLLNSVLNTLIMISVIVTMTIFLVVLYKYRCYKFIHGWLIMSSLMLLFLFTYIYLGEVLKTYNVAMDYPTLFLTVWNFGAVGMVCIHWKGPLVLQQAYLIMISALMALVFIKYLPEWSAWVILGAISVYDLVAVLCPKGPLRMLVETAQERNEPIFPALIYSSA | ||||||
Modified residue | 22 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 25 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 30 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 52 | Phosphoserine | ||||
Sequence: S | ||||||
Chain | PRO_0000025602 | 299-449 | Presenilin-2 CTF subunit | |||
Sequence: MVWTVGMAKLDPSSQGALQLPYDPEMEEDSYDSFGEPSYPDVFEPPLPGYPGEELEEEEERGVKLGLGDFIFYSVLVGKAAATGSGDWNTTLACFVAILIGLCLTLLLLAVFKKALPALPISITFGLIFYFSTDNLVRPFMDTLASHQLYI |
Post-translational modification
Phosphorylated on serine residues.
Keywords
- PTM
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
Homodimer. Component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. Such minimal complex is sufficient for secretase activity, although other components may exist. Interacts with DOCK3. Interacts with HERPUD1, FLNA and FLNB (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-71 | Disordered | ||||
Sequence: MLTFMASDSEEEVCDERTSLMSAESPTPRSCQDGRQGLEDGESAAQWRSQESEEDHEEEDPDRYVCSGVPG | ||||||
Motif | 415-417 | PAL | ||||
Sequence: PAL |
Domain
The PAL motif is required for normal active site conformation.
Sequence similarities
Belongs to the peptidase A22A family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length449
- Mass (Da)50,271
- Last updated2006-10-03 v2
- Checksum63080D05B70C1A6A
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0AAF6ZU55 | A0AAF6ZU55_BOVIN | PSEN2 | 416 | ||
A0A3Q1MPE8 | A0A3Q1MPE8_BOVIN | PSEN2 | 415 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 381 | in Ref. 1; AAD39024 | ||||
Sequence: T → M |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF038937 EMBL· GenBank· DDBJ | AAD39024.1 EMBL· GenBank· DDBJ | mRNA | ||
BC112811 EMBL· GenBank· DDBJ | AAI12812.1 EMBL· GenBank· DDBJ | mRNA |