Q9XI90 · WRKY4_ARATH
- ProteinProbable WRKY transcription factor 4
- GeneWRKY4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids514 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcription factor that binds specifically to the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Has a positive role in resistance to necrotrophic pathogens (e.g. Botrytis cinerea), but a negative effect on plant resistance to biotrophic pathogens (e.g. Pseudomonas syringae).
Features
Showing features for dna binding, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 223-287 | WRKY 1 | ||||
Sequence: NVDKPADDGYNWRKYGQKQVKGSEFPRSYYKCTNPGCPVKKKVERSLDGQVTEIIYKGQHNHEPP | ||||||
DNA binding | 403-468 | WRKY 2 | ||||
Sequence: SEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCGVRKHVERAATDPKAVVTTYEGKHNHDLP | ||||||
Binding site | 434 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 436 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 439 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 463 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 465 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | DNA binding | |
Molecular Function | DNA-binding transcription factor activity | |
Molecular Function | metal ion binding | |
Molecular Function | sequence-specific DNA binding | |
Molecular Function | transcription cis-regulatory region binding | |
Biological Process | defense response | |
Biological Process | negative regulation of defense response to bacterium | |
Biological Process | regulation of defense response to fungus | |
Biological Process | response to ethylene | |
Biological Process | response to jasmonic acid | |
Biological Process | response to salicylic acid |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameProbable WRKY transcription factor 4
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9XI90
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Increased sensitivity to the necrotrophic fungal pathogen Botrytis cinerea.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 25 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000133647 | 1-514 | Probable WRKY transcription factor 4 | |||
Sequence: MSEKEEAPSTSKSTGAPSRPTLSLPPRPFSEMFFNGGVGFSPGPMTLVSNMFPDSDEFRSFSQLLAGAMSSPATAAAAAAAATASDYQRLGEGTNSSSGDVDPRFKQNRPTGLMISQSQSPSMFTVPPGLSPAMLLDSPSFLGLFSPVQGSYGMTHQQALAQVTAQAVQANANMQPQTEYPPPSQVQSFSSGQAQIPTSAPLPAQRETSDVTIIEHRSQQPLNVDKPADDGYNWRKYGQKQVKGSEFPRSYYKCTNPGCPVKKKVERSLDGQVTEIIYKGQHNHEPPQNTKRGNKDNTANINGSSINNNRGSSELGASQFQTNSSNKTKREQHEAVSQATTTEHLSEASDGEEVGNGETDVREKDENEPDPKRRSTEVRISEPAPAASHRTVTEPRIIVQTTSEVDLLDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCGVRKHVERAATDPKAVVTTYEGKHNHDLPAAKSSSHAAAAAQLRPDNRPGGLANLNQQQQQQPVARLRLKEEQTT |
Proteomic databases
PTM databases
Expression
Tissue specificity
In young, mature and senescent leaves.
Induction
By biotic and abiotic stresses such as pathogen infection (e.g. Botrytis cinerea and Pseudomonas syringae), salicylic acid (SA), jasmonic acid (JA), ethylene (ACC), liquid infiltration or spraying, and strongly during leaf senescence.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-28 | Disordered | ||||
Sequence: MSEKEEAPSTSKSTGAPSRPTLSLPPRP | ||||||
Region | 175-204 | Disordered | ||||
Sequence: QPQTEYPPPSQVQSFSSGQAQIPTSAPLPA | ||||||
Region | 278-394 | Disordered | ||||
Sequence: YKGQHNHEPPQNTKRGNKDNTANINGSSINNNRGSSELGASQFQTNSSNKTKREQHEAVSQATTTEHLSEASDGEEVGNGETDVREKDENEPDPKRRSTEVRISEPAPAASHRTVTE | ||||||
Compositional bias | 292-326 | Polar residues | ||||
Sequence: RGNKDNTANINGSSINNNRGSSELGASQFQTNSSN | ||||||
Compositional bias | 346-379 | Basic and acidic residues | ||||
Sequence: SEASDGEEVGNGETDVREKDENEPDPKRRSTEVR | ||||||
Region | 464-514 | Disordered | ||||
Sequence: NHDLPAAKSSSHAAAAAQLRPDNRPGGLANLNQQQQQQPVARLRLKEEQTT | ||||||
Compositional bias | 490-514 | Polar residues | ||||
Sequence: GLANLNQQQQQQPVARLRLKEEQTT |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q9XI90-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length514
- Mass (Da)55,816
- Last updated2002-05-15 v2
- Checksum01010F8745C420C5
Q9XI90-2
- Name2
- Differences from canonical
- 112-147: GLMISQSQSPSMFTVPPGLSPAMLLDSPSFLGLFSP → AVLDLICNI
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A2H1ZEB2 | A0A2H1ZEB2_ARATH | WRKY4 | 361 |
Sequence caution
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_008969 | 112-147 | in isoform 2 | |||
Sequence: GLMISQSQSPSMFTVPPGLSPAMLLDSPSFLGLFSP → AVLDLICNI | ||||||
Compositional bias | 292-326 | Polar residues | ||||
Sequence: RGNKDNTANINGSSINNNRGSSELGASQFQTNSSN | ||||||
Compositional bias | 346-379 | Basic and acidic residues | ||||
Sequence: SEASDGEEVGNGETDVREKDENEPDPKRRSTEVR | ||||||
Compositional bias | 490-514 | Polar residues | ||||
Sequence: GLANLNQQQQQQPVARLRLKEEQTT |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF224703 EMBL· GenBank· DDBJ | AAK28313.1 EMBL· GenBank· DDBJ | mRNA | ||
AF425835 EMBL· GenBank· DDBJ | AAL13048.1 EMBL· GenBank· DDBJ | mRNA | ||
AC007576 EMBL· GenBank· DDBJ | AAD39282.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AC068197 EMBL· GenBank· DDBJ | AAF79402.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002684 EMBL· GenBank· DDBJ | AEE29089.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY045676 EMBL· GenBank· DDBJ | AAK74034.1 EMBL· GenBank· DDBJ | mRNA | ||
BT002629 EMBL· GenBank· DDBJ | AAO11545.1 EMBL· GenBank· DDBJ | mRNA |