Q9XEX2 · PRX2B_ARATH
- ProteinPeroxiredoxin-2B
- GenePRXIIB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids162 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin or glutaredoxin system. May be involved in intracellular redox signaling.
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this 1-Cys peroxiredoxin, no C(R) is present and C(P) instead forms a disulfide with a cysteine from another protein or with a small thiol molecule. C(P) is reactivated by glutaredoxin (Grx).
Catalytic activity
- [glutaredoxin]-dithiol + a hydroperoxide = [glutaredoxin]-disulfide + an alcohol + H2O
RHEA-COMP:10729 CHEBI:29950
Position: nCHEBI:29950
Position: n+3+ CHEBI:35924 = RHEA-COMP:10730 CHEBI:50058
Position: n/n+3+ CHEBI:30879 + CHEBI:15377
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 51 | Cysteine sulfenic acid (-SOH) intermediate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Cellular Component | plasma membrane | |
Molecular Function | thioredoxin peroxidase activity | |
Biological Process | cellular response to oxidative stress |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended namePeroxiredoxin-2B
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9XEX2
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000282279 | 1-162 | Peroxiredoxin-2B | ||
Proteomic databases
Expression
Tissue specificity
Expressed in all tissues but mostly in reproductive tissues such as buds, flowers, siliques and seeds.
Induction
Slightly induced by salt stress.
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 4-162 | Thioredoxin | |||
Sequence similarities
Belongs to the peroxiredoxin family. Prx5 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
Q9XEX2-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length162
- Mass (Da)17,428
- Last updated1999-11-01 v1
- MD5 ChecksumF9D2BD24A3486D23643C2ECF119F0C65
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F4ID64 | F4ID64_ARATH | TPX1 | 87 |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF121355 EMBL· GenBank· DDBJ | AAD28242.1 EMBL· GenBank· DDBJ | mRNA | ||
AC009513 EMBL· GenBank· DDBJ | AAF06058.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE34447.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF332464 EMBL· GenBank· DDBJ | AAG48827.1 EMBL· GenBank· DDBJ | mRNA | ||
AY065056 EMBL· GenBank· DDBJ | AAL57690.1 EMBL· GenBank· DDBJ | mRNA |