Q9XDP2 · ASST_LELAM
- ProteinArylsulfate sulfotransferase AssT
- GeneastA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids598 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the transfer of a sulfate group from a phenyl sulfate ester to other phenolic compounds. Is able to use several substrate donors and acceptors in vitro: using phenol as an acceptor substrate, 4-methylumbelliferyl sulfate is the best donor substrate, followed by beta-naphthyl sulfate, p-nitrophenyl sulfate (PNS), and alpha-naphthyl sulfate; using PNS as a donor substrate, alpha-naphthol is the best acceptor substrate, followed by phenol, resorcinol, p-acetaminophen, tyramine, and tyrosine. Cannot use 3'-phosphoadenosine-5'-phophosulfate (PAPS), the donor substrate of mammalian sulfotransferase. May be a detoxifying enzyme, converting toxic phenolic compounds into non-toxic materials.
Catalytic activity
- a phenol + an aryl sulfate = a phenol + an aryl sulfate
- 4-methylumbelliferone sulfate + phenol = 4-methylumbelliferone + phenyl sulfate
- 2-naphthyl sulfate + phenol = 2-naphthol + phenyl sulfate
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.163 mM | p-nitrophenyl sulfate | |||||
0.314 mM | phenol |
pH Dependence
Optimum pH is 9.0 using p-nitrophenyl sulfate as a donor and phenol as an acceptor substrate.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 279 | 4-methylumbelliferone (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 383 | 4-methylumbelliferone (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 463 | Nucleophile; sulfurylated histidine covalent intermediate | ||||
Sequence: H | ||||||
Binding site | 463 | 4-methylumbelliferone (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | periplasmic space | |
Molecular Function | aryl sulfotransferase activity | |
Molecular Function | arylsulfate sulfotransferase activity |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameArylsulfate sulfotransferase AssT
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Lelliottia
Accessions
- Primary accessionQ9XDP2
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-27 | |||||
Sequence: MFHPYRKTLLSGTVALALGLFATGAIA | ||||||
Chain | PRO_5009019430 | 28-598 | Arylsulfate sulfotransferase AssT | |||
Sequence: AGFQPAQPAGKLGAIVVDPYGNAPLTALIELDSHTISDVKVTVHGKGEKGVPVSYSVGKQSLATYDGIPVFGLYQKHANKVTVEYTENGKAMKEDYVIQTSAIVNRYMDNRSISDLQKTKVIKVAPGFEDRLYLVNTHTFTPQGAEFHWHGEKDKNAGLLDAGPAAGALPFDIAPFTFVVDTEGEYRWWLDQDTFYDGHDMNINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLADHKLPRGFLDATHESIETVNGTVLLRVGKRNYRKEDGLHVHTIRDQIIEVDKSGRVIDVWDLTKILDPLRDSLLGALDAGAVCVNVDLEHAGQQAKLEPDTPFGDALGVGAERNWAHVNPIAYDAKDDAIILSSRHQGIVKIGSDKKVKWILAPAKGWNKQLASKLLKPVDSKGNPLTCNENGKCENTDFDFSYTQHTAWLTDKGTLTVFDNGDGRWLEQPALPSMKYSRFVEYKIDEKNGTVQPLWQYGKERGYDFYSPITSVIEYQKDRDTIFGFSGSINLFEVGQPTIGKINEIDYKTKDVKVEIDVLSDKPNQTHYRALLVHPQQMFK | ||||||
Disulfide bond | 445↔451 | |||||
Sequence: CNENGKC |
Keywords
- PTM
Interaction
Subunit
Monomer.
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length598
- Mass (Da)66,684
- Last updated1999-11-01 v1
- Checksum3019CD522D58E586
Keywords
- Technical term