Q9WZC1 · MIAB_THEMA

Function

function

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site10[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site46[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site79[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site150[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site154[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site157[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionN6-isopentenyladenosine methylthiotransferase activity
Biological ProcesstRNA methylthiolation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
  • EC number
  • Alternative names
    • (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
    • tRNA-i(6)A37 methylthiotransferase

Gene names

    • Name
      miaB
    • Ordered locus names
      TM_0653

Organism names

Accessions

  • Primary accession
    Q9WZC1

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis10Loss of activity.
Mutagenesis150Decreased iron content and loss of activity; when associated with A-154 and A-157.
Mutagenesis154Decreased iron content and loss of activity; when associated with A-150 and A-157.
Mutagenesis157Decreased iron content and loss of activity; when associated with A-150 and A-154.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003595901-443tRNA-2-methylthio-N6-dimethylallyladenosine synthase

Proteomic databases

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-114MTTase N-terminal
Domain136-367Radical SAM core
Domain370-431TRAM

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    443
  • Mass (Da)
    50,742
  • Last updated
    1999-11-01 v1
  • Checksum
    2C738B79667C9895
MRFYIKTFGCQMNENDSEAMAGLLVKEGFTPASSPEEADVVIINTCAVRRKSEEKAYSELGQVLKLKKKKKIVVGVAGCVAEKEREKFLEKGADFVLGTRAVPRVTEAVKKALEGEKVALFEDHLDEYTHELPRIRTSRHHAWVTIIHGCDRFCTYCIVPYTRGRERSRPMADILEEVKKLAEQGYREVTFLGQNVDAYGKDLKDGSSLAKLLEEASKIEGIERIWFLTSYPTDFSDELIEVIAKNPKVAKSVHLPVQSGSNRILKLMNRRYTKEEYLALLEKIRSKVPEVAISSDIIVGFPTETEEDFMETVDLVEKAQFERLNLAIYSPREGTVAWKYYKDDVPYEEKVRRMQFLMNLQKRINRKLNERYRGKTVRIIVEAQAKNGLFYGRDIRNKIIAFEGEDWMIGRFADVKVEKITAGPLYGKVVWVEKTPSPVSSSE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE000512
EMBL· GenBank· DDBJ
AAD35737.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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