Q9WXG7 · DPDD_ALCFA
- ProteinCis-3,4-dihydrophenanthrene-3,4-diol dehydrogenase
- GenephnB
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids277 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in the degradation of phenanthrene. Catalyzes the oxidation of cis-phenanthrene dihydrodiol (PDD) to yield phenanthrenediol. It can use either NAD or NADP as electron acceptor, however NAD is preferred to NADP.
Catalytic activity
- (3S,4R)-3,4-dihydrophenanthrene-3,4-diol + NAD+ = H+ + NADH + phenanthrene-3,4-diol
Activity regulation
Inhibited by heavy metal such as Hg2+ and by p-chloromercuribenzoate.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
210 μM | PDD | 9 | 30 | |||
580 μM | NAD | 9 | 30 | |||
6.3 mM | NADP | 9 | 30 |
pH Dependence
Optimum pH is 9.
Temperature Dependence
Optimum temperature is 40 degrees Celsius.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | cis-3,4-dihydrophenanthrene-3,4-diol dehydrogenase activity | |
Biological Process | phenanthrene catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCis-3,4-dihydrophenanthrene-3,4-diol dehydrogenase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Alcaligenaceae > Alcaligenes
Accessions
- Primary accessionQ9WXG7
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000424348 | 1-277 | Cis-3,4-dihydrophenanthrene-3,4-diol dehydrogenase | |||
Sequence: MAWLEGQSVFLTGGVAGLGRALVKRLVEEGANVTVLDRNARGLDELVESFKGRVAGSPGDVRNLADNRKAVELAVERFGKLDTFNRQRRHLGLLCPPCRPAGRCHQRSFDEVIGINLMGYVMGIKAAAPALVRSRGSVILTLSSSAFYAGGGGVLYTVAKHAAVGLIKQAAHELAPYVRVNGVAPGGIASDLRGPKSLGMGEQSITSVPLADLVKDIAPIGRLSDTEEYTGSYVYLASARNSAPATGVIINCDGGMGVRSVLGPASGGKGLLEKFGG |
Interaction
Subunit
Homotetramer.
Structure
Sequence
- Sequence statusComplete
- Length277
- Mass (Da)28,827
- Last updated1999-11-01 v1
- ChecksumDE3701AB9B96D7DC