Q9WUF4 · VAMP8_RAT

  • Protein
    Vesicle-associated membrane protein 8
  • Gene
    Vamp8
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (By similarity).
Also required for dense-granule secretion in platelets (By similarity).
Also plays a role in regulated enzyme secretion in pancreatic acinar cells (By similarity).
Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (PubMed:12737809).
Involved in the homotypic fusion of early and late endosomes (PubMed:10982406, PubMed:11029036).
Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism (By similarity).

Features

Showing features for site.

1100102030405060708090100
TypeIDPosition(s)Description
Site32Binding to STX8

GO annotations

AspectTerm
Cellular Componentazurophil granule membrane
Cellular Componentbasal part of cell
Cellular Componentbasolateral part of cell
Cellular Componentbasolateral plasma membrane
Cellular Componentcell surface
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentearly endosome membrane
Cellular Componentlate endosome membrane
Cellular Componentlysosomal membrane
Cellular Componentlysosome
Cellular Componentmembrane
Cellular Componentmidbody
Cellular Componentmucin granule
Cellular Componentperinuclear region of cytoplasm
Cellular Componentphagocytic vesicle
Cellular Componentplasma membrane
Cellular Componentrecycling endosome
Cellular Componentsecretory granule
Cellular Componentsecretory granule membrane
Cellular ComponentSNARE complex
Cellular Componentvesicle
Cellular Componentzymogen granule
Cellular Componentzymogen granule membrane
Molecular Functionchloride channel inhibitor activity
Molecular FunctionSNAP receptor activity
Molecular Functionsyntaxin binding
Biological Processautophagosome maturation
Biological Processautophagosome membrane docking
Biological Processcellular response to type II interferon
Biological Processdefense response to virus
Biological Processestablishment of localization in cell
Biological Processmembrane fusion
Biological Processmucus secretion
Biological Processnegative regulation of secretion by cell
Biological Processpositive regulation of histamine secretion by mast cell
Biological Processpositive regulation of pancreatic amylase secretion
Biological Processprotein transport
Biological Processprotein-containing complex assembly
Biological Processregulation of endocytosis
Biological Processregulation of protein localization to plasma membrane
Biological ProcessSNARE complex assembly
Biological Processsymbiont entry into host cell
Biological Processvesicle fusion
Biological Processvesicle-mediated transport
Biological Processzymogen granule exocytosis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Vesicle-associated membrane protein 8
  • Short names
    VAMP-8
  • Alternative names
    • Endobrevin
      (EDB
      )

Gene names

    • Name
      Vamp8

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    Q9WUF4

Proteomes

Organism-specific databases

Subcellular Location

Lysosome membrane
; Single-pass type IV membrane protein
Late endosome membrane
; Single-pass type IV membrane protein
Early endosome membrane
; Single-pass type IV membrane protein
Midbody
Cell membrane
; Single-pass type IV membrane protein
Zymogen granule membrane
; Single-pass type IV membrane protein
Note: Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi (PubMed:9614193).
Sub-tight junctional domain in retinal pigment epithelium cells (PubMed:11029036).
Midbody region during cytokinesis (PubMed:12414999).
Lumenal oriented, apical membranes of nephric tubular cell (PubMed:11208143).
Cycles through the apical but not through the basolateral plasma membrane (By similarity).
Apical region of acinar cells; in zymogen granule membranes (By similarity).

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-74Cytoplasmic
Transmembrane75-95Helical; Anchor for type IV membrane protein
Topological domain96-100Vesicular

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00002067391-100Vesicle-associated membrane protein 8
Modified residue4Phosphoserine
Modified residue17Phosphoserine
Modified residue27Phosphothreonine
Modified residue47Phosphothreonine
Modified residue53Phosphothreonine
Modified residue54Phosphoserine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed (at protein level) at a high level in kidney, lung and spleen; at a lower level in testis, liver, brain and heart. Expressed in kidney and retinal pigment epithelium derived cell line.

Interaction

Subunit

Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome (By similarity).
Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (PubMed:10336434, PubMed:11786915, PubMed:9614193).
Interacts with PICALM (By similarity).
SNARE complex formation and binding by PICALM are mutually exclusive processes for VAMP8 (By similarity).
Interacts with SBF2/MTMR13 (By similarity).
Interacts with RAB21 (in GTP-bound form) in response to starvation; the interaction probably regulates VAMP8 endolysosomal trafficking (By similarity).
Interacts with STX17; this interaction is increased in the absence of TMEM39A (By similarity).
Interacts with TRIM6 (By similarity).

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain11-71v-SNARE coiled-coil homology

Sequence similarities

Belongs to the synaptobrevin family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    100
  • Mass (Da)
    11,320
  • Last updated
    1999-11-01 v1
  • Checksum
    D4449EAF44F03672
MEASGSAGNDRVRNLQSEVEGVKNIMTQNVERILARGENLDHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVIICVIVLIILILIILFATGTIPT

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
F1LNT8F1LNT8_RATVamp8116
A0A8I6AB54A0A8I6AB54_RATVamp8100

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF132812
EMBL· GenBank· DDBJ
AAD33595.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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