Q9WUF4 · VAMP8_RAT
- ProteinVesicle-associated membrane protein 8
- GeneVamp8
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids100 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (By similarity).
Also required for dense-granule secretion in platelets (By similarity).
Also plays a role in regulated enzyme secretion in pancreatic acinar cells (By similarity).
Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (PubMed:12737809).
Involved in the homotypic fusion of early and late endosomes (PubMed:10982406, PubMed:11029036).
Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism (By similarity).
Also required for dense-granule secretion in platelets (By similarity).
Also plays a role in regulated enzyme secretion in pancreatic acinar cells (By similarity).
Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (PubMed:12737809).
Involved in the homotypic fusion of early and late endosomes (PubMed:10982406, PubMed:11029036).
Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism (By similarity).
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 32 | Binding to STX8 | ||||
Sequence: R |
GO annotations
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameVesicle-associated membrane protein 8
- Short namesVAMP-8
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ9WUF4
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Lysosome membrane ; Single-pass type IV membrane protein
Late endosome membrane ; Single-pass type IV membrane protein
Early endosome membrane ; Single-pass type IV membrane protein
Cell membrane ; Single-pass type IV membrane protein
Zymogen granule membrane ; Single-pass type IV membrane protein
Note: Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi (PubMed:9614193).
Sub-tight junctional domain in retinal pigment epithelium cells (PubMed:11029036).
Midbody region during cytokinesis (PubMed:12414999).
Lumenal oriented, apical membranes of nephric tubular cell (PubMed:11208143).
Cycles through the apical but not through the basolateral plasma membrane (By similarity).
Apical region of acinar cells; in zymogen granule membranes (By similarity).
Sub-tight junctional domain in retinal pigment epithelium cells (PubMed:11029036).
Midbody region during cytokinesis (PubMed:12414999).
Lumenal oriented, apical membranes of nephric tubular cell (PubMed:11208143).
Cycles through the apical but not through the basolateral plasma membrane (By similarity).
Apical region of acinar cells; in zymogen granule membranes (By similarity).
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-74 | Cytoplasmic | ||||
Sequence: MEASGSAGNDRVRNLQSEVEGVKNIMTQNVERILARGENLDHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVK | ||||||
Transmembrane | 75-95 | Helical; Anchor for type IV membrane protein | ||||
Sequence: MIVIICVIVLIILILIILFAT | ||||||
Topological domain | 96-100 | Vesicular | ||||
Sequence: GTIPT |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000206739 | 1-100 | Vesicle-associated membrane protein 8 | |||
Sequence: MEASGSAGNDRVRNLQSEVEGVKNIMTQNVERILARGENLDHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVIICVIVLIILILIILFATGTIPT | ||||||
Modified residue | 4 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 17 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 27 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 47 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 53 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 54 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed (at protein level) at a high level in kidney, lung and spleen; at a lower level in testis, liver, brain and heart. Expressed in kidney and retinal pigment epithelium derived cell line.
Interaction
Subunit
Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome (By similarity).
Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (PubMed:10336434, PubMed:11786915, PubMed:9614193).
Interacts with PICALM (By similarity).
SNARE complex formation and binding by PICALM are mutually exclusive processes for VAMP8 (By similarity).
Interacts with SBF2/MTMR13 (By similarity).
Interacts with RAB21 (in GTP-bound form) in response to starvation; the interaction probably regulates VAMP8 endolysosomal trafficking (By similarity).
Interacts with STX17; this interaction is increased in the absence of TMEM39A (By similarity).
Interacts with TRIM6 (By similarity).
Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (PubMed:10336434, PubMed:11786915, PubMed:9614193).
Interacts with PICALM (By similarity).
SNARE complex formation and binding by PICALM are mutually exclusive processes for VAMP8 (By similarity).
Interacts with SBF2/MTMR13 (By similarity).
Interacts with RAB21 (in GTP-bound form) in response to starvation; the interaction probably regulates VAMP8 endolysosomal trafficking (By similarity).
Interacts with STX17; this interaction is increased in the absence of TMEM39A (By similarity).
Interacts with TRIM6 (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 11-71 | v-SNARE coiled-coil homology | ||||
Sequence: RVRNLQSEVEGVKNIMTQNVERILARGENLDHLRNKTEDLEATSEHFKTTSQKVARKFWWK |
Sequence similarities
Belongs to the synaptobrevin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length100
- Mass (Da)11,320
- Last updated1999-11-01 v1
- ChecksumD4449EAF44F03672
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F1LNT8 | F1LNT8_RAT | Vamp8 | 116 | ||
A0A8I6AB54 | A0A8I6AB54_RAT | Vamp8 | 100 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF132812 EMBL· GenBank· DDBJ | AAD33595.1 EMBL· GenBank· DDBJ | mRNA |