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Q9WUB7 · CLCKA_MOUSE

  • Protein
    Chloride channel protein ClC-Ka
  • Gene
    Clcnka
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Anion-selective channel permeable to small monovalent anions with ion selectivity for chloride > bromide > nitrate > iodide (PubMed:23791703).
Forms a homodimeric channel where each subunit has its own ion conduction pathway. May conduct double-barreled currents controlled by two types of gates, two fast gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously (PubMed:23791703).
Assembles with the regulatory subunit BSND/Barttin for sorting at the basolateral plasma membrane domain and functional switch to the ion conducting state. CLCNKA:BSND channels display mostly a linear current-voltage relationship with fast gating at negative potentials (PubMed:23791703).
Mediates transepithelial chloride transport from the lumen to interstitial compartment along the thin ascending limb of Henle's loop, contributing to generation of hypertonic medullary interstitium as a countercurrent system to achieve urine concentration (PubMed:34499620, PubMed:9916798).
Conducts chloride currents in the stria vascularis of the inner ear to establish the endocochlear potential necessary for normal hearing (By similarity).

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site259Ca2+ (UniProtKB | ChEBI)
Binding site261Ca2+ (UniProtKB | ChEBI)
Binding site278Ca2+ (UniProtKB | ChEBI)
Binding site281Ca2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentbasolateral plasma membrane
Cellular Componentchloride channel complex
Molecular Functionchloride channel activity
Molecular Functionmetal ion binding
Molecular Functionvoltage-gated chloride channel activity
Biological Processregulation of body fluid levels
Biological Processrenal inner medulla development
Biological Processtransepithelial chloride transport

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Chloride channel protein ClC-Ka
  • Short names
    Chloride channel Ka
  • Alternative names
    • ClC-K1

Gene names

    • Name
      Clcnka
    • Synonyms
      Clcnk1

Organism names

  • Taxonomic identifier
  • Strains
    • C57BL/6J
    • FVB/N
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q9WUB7
  • Secondary accessions
    • A2ADB3
    • Q6UB69
    • Q8JZU7

Proteomes

Organism-specific databases

Subcellular Location

Features

Showing features for transmembrane, topological domain.

Type
IDPosition(s)Description
Transmembrane52-72Helical
Transmembrane94-114Helical
Transmembrane161-181Helical
Transmembrane204-224Helical
Transmembrane236-256Helical
Transmembrane282-302Helical
Transmembrane325-345Helical
Transmembrane396-416Helical
Transmembrane417-437Helical
Transmembrane458-478Helical
Transmembrane486-506Helical
Topological domain507-687Cytoplasmic

Keywords

Phenotypes & Variants

Disruption phenotype

Mice are born at the expected Mendelian ratios. They develop nephrogenic diabetes insipidus associated with polyuria and water diuresis.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis166Reduces the unit conductance from 40 pS to around 20 pS; displays outward rectification with half maximal activation voltage shifted to more positive potentials.
Mutagenesis166No significant effect on the unit conductance.
Mutagenesis166Moderately reduces the unit conductance.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 21 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000944561-687Chloride channel protein ClC-Ka

Proteomic databases

PTM databases

Expression

Tissue specificity

Specifically expressed in the kidney, predominantly in the inner medulla. All nephron segments expressing BSND also express CLCNK proteins.

Developmental stage

Expressed in embryonic kidneys, first detected at the nephrogenic zone around 18.5 dpc, being diffusely expressed in the renal cortex and medulla including the loop of Henle. Preferentially localizes to the renal medulla by P1.

Gene expression databases

Interaction

Subunit

Homodimer. Interacts with BSND.

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain551-609CBS 1
Domain626-687CBS 2

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    687
  • Mass (Da)
    75,684
  • Last updated
    2011-07-27 v2
  • MD5 Checksum
    AAA0F72205D4A44E3FA28EE3EC5CEED0
MEELVGLREGSSGKPVTLQELWGPCPRIRRGIRGGLEWLKERLFRAREDWYFLVALGVLMALISYAMNFAIGRVVRAHKWLYREVGDGHLLRYLSWTVYPVALLSFSSGFSQSITPFSGGSGLPELKTMLSGVVLENYLDIKNFGAKVVGLSCTLATGSTIFLGKVGPFVHLSVMISAYLGRVRAKAVGDTESKAKEVEMLSAAAAVGVATVFAAPFSGVLFSIEVMSSHFSVWNYWRGFFAATCGAFMFRLLAVFNSEQETITSIYKTRFRVDVPFDLPEIFFFVALGFICGILSCVYLFCQRNFLRFIKTNRYTSKLLATSKPSYAALVALVLASVTYPPGVGRFMASRLSMAEHLHSLFDNNSWALMTRNASPPWPSEPDPQNLWFEWYHPRFTIFGTLAFFLIMKFWMLILATTIPMPAGYFMPIFIIGAAIGRLLGEALSVAFPEGIVAGGEVYPIMPGGYALAGAAAFSGAVTHTISTALLAFELTGQIVHALPVLMAVLAANVISQNLQPSFYDGTIMAKKLPYLPWIRGRQIGSYPVTVEHFMNSNLTTLAKDMPLEEVVKVVTSTDVSQYPLVETRESQTLVGIVERTHLVQALQTQPASWAPGQERFLQDILAGGCPTQPVTLQLSPETSLYQAHRLFELLTLQALFVTSRGRAVGSVSWVELKKAISTLINPPAPK

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A2ADB4A2ADB4_MOUSEClcnka192

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict372in Ref. 4; AAH37077
Sequence conflict380in Ref. 1; AAD21083 and 4; AAH37077

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF124848
EMBL· GenBank· DDBJ
AAD21083.1
EMBL· GenBank· DDBJ
mRNA
AY373832
EMBL· GenBank· DDBJ
AAQ81628.1
EMBL· GenBank· DDBJ
mRNA
AL670285
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC037077
EMBL· GenBank· DDBJ
AAH37077.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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