Q9WUB7 · CLCKA_MOUSE
- ProteinChloride channel protein ClC-Ka
- GeneClcnka
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids687 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Anion-selective channel permeable to small monovalent anions with ion selectivity for chloride > bromide > nitrate > iodide (PubMed:23791703).
Forms a homodimeric channel where each subunit has its own ion conduction pathway. May conduct double-barreled currents controlled by two types of gates, two fast gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously (PubMed:23791703).
Assembles with the regulatory subunit BSND/Barttin for sorting at the basolateral plasma membrane domain and functional switch to the ion conducting state. CLCNKA:BSND channels display mostly a linear current-voltage relationship with fast gating at negative potentials (PubMed:23791703).
Mediates transepithelial chloride transport from the lumen to interstitial compartment along the thin ascending limb of Henle's loop, contributing to generation of hypertonic medullary interstitium as a countercurrent system to achieve urine concentration (PubMed:34499620, PubMed:9916798).
Conducts chloride currents in the stria vascularis of the inner ear to establish the endocochlear potential necessary for normal hearing (By similarity).
Forms a homodimeric channel where each subunit has its own ion conduction pathway. May conduct double-barreled currents controlled by two types of gates, two fast gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously (PubMed:23791703).
Assembles with the regulatory subunit BSND/Barttin for sorting at the basolateral plasma membrane domain and functional switch to the ion conducting state. CLCNKA:BSND channels display mostly a linear current-voltage relationship with fast gating at negative potentials (PubMed:23791703).
Mediates transepithelial chloride transport from the lumen to interstitial compartment along the thin ascending limb of Henle's loop, contributing to generation of hypertonic medullary interstitium as a countercurrent system to achieve urine concentration (PubMed:34499620, PubMed:9916798).
Conducts chloride currents in the stria vascularis of the inner ear to establish the endocochlear potential necessary for normal hearing (By similarity).
Catalytic activity
- chloride(in) = chloride(out)
- bromide(in) = bromide(out)
- nitrate(in) = nitrate(out)
- iodide(out) = iodide(in)
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 259 | Ca2+ (UniProtKB | ChEBI) | |||
Binding site | 261 | Ca2+ (UniProtKB | ChEBI) | |||
Binding site | 278 | Ca2+ (UniProtKB | ChEBI) | |||
Binding site | 281 | Ca2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | basolateral plasma membrane | |
Cellular Component | chloride channel complex | |
Molecular Function | chloride channel activity | |
Molecular Function | metal ion binding | |
Molecular Function | voltage-gated chloride channel activity | |
Biological Process | regulation of body fluid levels | |
Biological Process | renal inner medulla development | |
Biological Process | transepithelial chloride transport |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameChloride channel protein ClC-Ka
- Short namesChloride channel Ka
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ9WUB7
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Basolateral cell membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane, topological domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 52-72 | Helical | |||
Transmembrane | 94-114 | Helical | |||
Transmembrane | 161-181 | Helical | |||
Transmembrane | 204-224 | Helical | |||
Transmembrane | 236-256 | Helical | |||
Transmembrane | 282-302 | Helical | |||
Transmembrane | 325-345 | Helical | |||
Transmembrane | 396-416 | Helical | |||
Transmembrane | 417-437 | Helical | |||
Transmembrane | 458-478 | Helical | |||
Transmembrane | 486-506 | Helical | |||
Topological domain | 507-687 | Cytoplasmic | |||
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Mice are born at the expected Mendelian ratios. They develop nephrogenic diabetes insipidus associated with polyuria and water diuresis.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 166 | Reduces the unit conductance from 40 pS to around 20 pS; displays outward rectification with half maximal activation voltage shifted to more positive potentials. | |||
Mutagenesis | 166 | No significant effect on the unit conductance. | |||
Mutagenesis | 166 | Moderately reduces the unit conductance. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 21 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000094456 | 1-687 | Chloride channel protein ClC-Ka | ||
Proteomic databases
PTM databases
Expression
Tissue specificity
Specifically expressed in the kidney, predominantly in the inner medulla. All nephron segments expressing BSND also express CLCNK proteins.
Developmental stage
Expressed in embryonic kidneys, first detected at the nephrogenic zone around 18.5 dpc, being diffusely expressed in the renal cortex and medulla including the loop of Henle. Preferentially localizes to the renal medulla by P1.
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 551-609 | CBS 1 | |||
Domain | 626-687 | CBS 2 | |||
Sequence similarities
Belongs to the chloride channel (TC 2.A.49) family. CLCNKA subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length687
- Mass (Da)75,684
- Last updated2011-07-27 v2
- MD5 ChecksumAAA0F72205D4A44E3FA28EE3EC5CEED0
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A2ADB4 | A2ADB4_MOUSE | Clcnka | 192 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 372 | in Ref. 4; AAH37077 | |||
Sequence conflict | 380 | in Ref. 1; AAD21083 and 4; AAH37077 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF124848 EMBL· GenBank· DDBJ | AAD21083.1 EMBL· GenBank· DDBJ | mRNA | ||
AY373832 EMBL· GenBank· DDBJ | AAQ81628.1 EMBL· GenBank· DDBJ | mRNA | ||
AL670285 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC037077 EMBL· GenBank· DDBJ | AAH37077.1 EMBL· GenBank· DDBJ | mRNA |