Q9WU56 · PUS1_MOUSE

  • Protein
    Pseudouridylate synthase 1 homolog
  • Gene
    Pus1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Pseudouridylate synthase that catalyzes pseudouridylation of tRNAs and mRNAs (PubMed:10094309, PubMed:15327771, PubMed:27197761).
Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA (PubMed:10094309).
Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (By similarity).
Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions (By similarity).
Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing (By similarity).
Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 mRNA (PubMed:15327771).

Isoform 3

Does not form pseudouridine when expressed in vitro.

Isoform 4

Does not form pseudouridine when expressed in vitro.

Catalytic activity

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site142Nucleophile

GO annotations

AspectTerm
Cellular Componentmitochondrion
Cellular Componentnucleolus
Cellular Componentnucleus
Cellular Componenttranscription regulator complex
Molecular Functionchromatin binding
Molecular Functionnuclear receptor coactivator activity
Molecular Functionpseudouridine synthase activity
Molecular Functionsteroid receptor RNA activator RNA binding
Molecular FunctiontRNA binding
Molecular FunctiontRNA pseudouridine synthase activity
Molecular FunctiontRNA pseudouridine(38-40) synthase activity
Biological ProcessmRNA processing
Biological ProcessmRNA pseudouridine synthesis
Biological Processpositive regulation of transcription by RNA polymerase II
Biological ProcessRNA splicing
Biological ProcesstRNA pseudouridine synthesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pseudouridylate synthase 1 homolog
  • EC number
  • Alternative names
    • tRNA pseudouridine synthase 1
      (EC:5.4.99.12
      ) . EC:5.4.99.12 (UniProtKB | ENZYME | Rhea)
    • tRNA pseudouridine(38-40) synthase
    • tRNA pseudouridylate synthase I
    • tRNA-uridine isomerase I

Gene names

    • Name
      Pus1
    • ORF names
      MNCb-0873

Organism names

  • Taxonomic identifier
  • Strains
    • 129/SvJ
    • C57BL/6J
    • NOD
    • Czech II
    • FVB/N
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q9WU56
  • Secondary accessions
    • Q3UUC6
    • Q791J1
    • Q8C250
    • Q8VDQ3
    • Q9EQD1
    • Q9JJE5

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Impaired muscle morphology and physiology leading to impaired exercise capacity (PubMed:27197761).
Mice were born at the expected Mendelian frequency (PubMed:27197761).
At 14 weeks, mice display reduced exercise capacity, probably caused by alterations in muscle metabolism related to mitochondrial content and oxidative capacity (PubMed:27197761).
Cells show reduced pseudouridylation of cytoplasmic and mitochondrial tRNAs (PubMed:27197761).

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 12 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, transit peptide, modified residue.

TypeIDPosition(s)Description
ChainPRO_0000057518?-423Pseudouridylate synthase 1 homolog
Transit peptide1-?Mitochondrion
Modified residue411Phosphoserine
Modified residue416Phosphoserine
Modified residue422Phosphothreonine

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Monomer (By similarity).
Forms a complex with RARG and the SRA1 RNA in the nucleus (PubMed:15327771).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region32-75Disordered
Compositional bias49-75Basic and acidic residues
Region403-423Disordered

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (4)
  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 4 isoforms produced by Alternative splicing.

Q9WU56-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    423
  • Mass (Da)
    47,502
  • Last updated
    2006-09-05 v2
  • Checksum
    C71FDBF60F29295A
MGFPRLWAALLRNWGRWTARPGPRVPGLPPMAGNKVPPALASHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKDRDVQDESYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNFSGTALGAADTGAKVPSSLEGSEGDGDTD

Q9WU56-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Q9WU56-3

  • Name
    3
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Q9WU56-4

  • Name
    4
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
H7BX59H7BX59_MOUSEPus1441
D3YWU8D3YWU8_MOUSEPus1147
D3Z092D3Z092_MOUSEPus1162

Sequence caution

The sequence BAA95047.1 differs from that shown. Reason: Frameshift
The sequence BAC40817.1 differs from that shown. Reason: Erroneous initiation

Features

Showing features for alternative sequence, sequence conflict, compositional bias.

TypeIDPosition(s)Description
Alternative sequenceVSP_0201141-30in isoform 2, isoform 3 and isoform 4
Sequence conflict48in Ref. 4; BAC40817
Compositional bias49-75Basic and acidic residues
Sequence conflict52in Ref. 4; BAC40817
Alternative sequenceVSP_02179198-143in isoform 4
Alternative sequenceVSP_021792143in isoform 3
Sequence conflict406in Ref. 5; AAH21446

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF116237
EMBL· GenBank· DDBJ
AAD21041.1
EMBL· GenBank· DDBJ
mRNA
AF269250
EMBL· GenBank· DDBJ
AAG35307.1
EMBL· GenBank· DDBJ
Genomic DNA
AF269250
EMBL· GenBank· DDBJ
AAG35308.1
EMBL· GenBank· DDBJ
Genomic DNA
AB041563
EMBL· GenBank· DDBJ
BAA95047.1
EMBL· GenBank· DDBJ
mRNA Frameshift
AK089258
EMBL· GenBank· DDBJ
BAC40817.1
EMBL· GenBank· DDBJ
mRNA Different initiation
AK138570
EMBL· GenBank· DDBJ
BAE23701.1
EMBL· GenBank· DDBJ
mRNA
BC021446
EMBL· GenBank· DDBJ
AAH21446.2
EMBL· GenBank· DDBJ
mRNA
BC034359
EMBL· GenBank· DDBJ
AAH34359.2
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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