Q9W484 · RTEL1_DROME
- ProteinRegulator of telomere elongation helicase 1 homolog
- GeneRtel1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids985 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
A probable ATP-dependent DNA helicase implicated in DNA repair and the maintenance of genomic stability. Acts as an anti-recombinase to counteract toxic recombination and limit crossover during meiosis. Regulates meiotic recombination and crossover homeostasis by physically dissociating strand invasion events and thereby promotes noncrossover repair by meiotic synthesis dependent strand annealing (SDSA) as well as disassembly of D loop recombination intermediates (By similarity).
In male germline stem cells (GSCs), plays a role in GSCs maintenance during larval germline development by modulating the expression of genes such as Stat92E and preventing DNA damage-induced checkpoint activation (PubMed:34644293).
May play a role in female germline stem cell maintenance (PubMed:34644293).
In male germline stem cells (GSCs), plays a role in GSCs maintenance during larval germline development by modulating the expression of genes such as Stat92E and preventing DNA damage-induced checkpoint activation (PubMed:34644293).
May play a role in female germline stem cell maintenance (PubMed:34644293).
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 42-49 | ATP (UniProtKB | ChEBI) | ||||
Sequence: SPTGTGKT | ||||||
Binding site | 146 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 164 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 173 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 209 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome | |
Cellular Component | nucleus | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | chromatin binding | |
Molecular Function | DNA binding | |
Molecular Function | DNA helicase activity | |
Molecular Function | DNA polymerase binding | |
Molecular Function | metal ion binding | |
Biological Process | DNA damage response | |
Biological Process | DNA duplex unwinding | |
Biological Process | DNA recombination | |
Biological Process | DNA repair | |
Biological Process | DNA replication | |
Biological Process | male germ-line stem cell population maintenance | |
Biological Process | negative regulation of DNA recombination | |
Biological Process | negative regulation of t-circle formation | |
Biological Process | regulation of double-strand break repair via homologous recombination | |
Biological Process | telomeric loop disassembly |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRegulator of telomere elongation helicase 1 homolog
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ9W484
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
In males, RNAi-mediated knockdown in germ cells results in loss of male germline stem cells (GSCs) at day 14, appearance of branched fusomes and down regulation of a number of genes including Stat92E expression in adult testes (PubMed:34644293).
In females, RNAi-mediated knockdown in germ cells results in a decrease in germline stem cells in the germaria (PubMed:34644293).
RNAi-mediated knockdown in somatic cells or in differentiating spermatogonia does not affect GSC number in larval and adult testes or female germaria (PubMed:34644293).
In the germline, simultaneous RNAi-mediated knockdown of Rtel1 and grp results in partial rescue of loss of germline stem cell (PubMed:34644293).
In the germline, RNAi-mediated knockdown of Rtel1 in a lok mutant background results in partial rescue of loss of germline stem cell, including restored levels of Stat92E expression (PubMed:34644293).
In females, RNAi-mediated knockdown in germ cells results in a decrease in germline stem cells in the germaria (PubMed:34644293).
RNAi-mediated knockdown in somatic cells or in differentiating spermatogonia does not affect GSC number in larval and adult testes or female germaria (PubMed:34644293).
In the germline, simultaneous RNAi-mediated knockdown of Rtel1 and grp results in partial rescue of loss of germline stem cell (PubMed:34644293).
In the germline, RNAi-mediated knockdown of Rtel1 in a lok mutant background results in partial rescue of loss of germline stem cell, including restored levels of Stat92E expression (PubMed:34644293).
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 81 | in strain: ZW104 | ||||
Sequence: G → D | ||||||
Natural variant | 95 | in strain: ZW141 | ||||
Sequence: A → T | ||||||
Natural variant | 294 | in strain: ZW104 and ZW141 | ||||
Sequence: Missing |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 3 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000370623 | 1-985 | Regulator of telomere elongation helicase 1 homolog | |||
Sequence: MPESLIAGIPVHFPFEPYPVQRAYMEKVIHCLRDGTNGVLESPTGTGKTLSLLCSSLAWIRTRQSEHQKQMVKMEKADFSGLGGGAPGGDLSELAKTMGRANNWGVPKVIYASRTHSQLTQAMRELKRTAYANMRSVVLGSRDQLCIHPEVMREQGNSNKTNMCKLRVHSKTCSFQMRVESRKDHPDLRGPTIMDIEDLVKVGQRLKICPYFASRELVPQADITFMPYNYLLDPKARKANKIELGNTIVILDEAHNIEKICEESASVQIKSSDVAMAIEDVTHIMQVFASGESQDMAGDEPKDFTLDDLTLLKEMLLELEKAIDAIVVDNAVDGTTFPASMMYELLGKANFTYGNVATIVSLLDKLVQYLLVASQQMSIRKGGTFTLLSDLLTIVFANKEDVMSKVYASFKVHVLVEESKQGHGKQQGAKQQGGWLGKGTIAAATGLSKVAKIINFWCFNPGFGMEQLLNTQVRSVILTSGTLAPLKPLIAELAIPVAQHLENPHIVDQSQVYVKIIGTGPDRQQLISNYANRDNPKYISSLGQTILNVARIVPDGLLVFFPSYPMLNKCVDAWQASGLWADISCKKPIFLEPRSKDQFTSTMEEFYQAIRDSKGAVFMAVCRGKVSEGLDFADRNGRAVIITGLPFPPLKDPKVILKRRYLEANRTRENQLLSGQEWYNLDATRAVNQAIGRVIRHRNDYGAILLCDSRFKDASQVQQLSKWIRGHLGDRPQCSPFGPIVRELRQFFKNAEANMKLPDERETDSPLETVCKTEDEPLAAIPKVKREPGSNATFKSANESAIKVEMANSIKTWTPADYASAAGHKLGGAAPNAMDFMSRLDSNVSSIDFNCCTDSKSGSSGLVKIHKRERSSPTAPESSSQVTKKRYKLVENIKVEPSSSQAKEAPEERAAFLRELRSLVTQDQFRRFGKALLEYKNGTYESFQALMAILLDVLSAPKVRYMLVGMRKYLKNEHKDEFDRRVGNL | ||||||
Modified residue | 874 | Phosphothreonine | ||||
Sequence: T |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in both male germline and somatic cells (at protein level) (PubMed:34644293).
Expressed in ovarian germline stem cells (at protein level) (PubMed:34644293).
Expressed in adult testes (at protein level) (PubMed:34644293).
Expressed in the germarium including germline stem cells (PubMed:34644293).
Expressed in ovarian germline stem cells (at protein level) (PubMed:34644293).
Expressed in adult testes (at protein level) (PubMed:34644293).
Expressed in the germarium including germline stem cells (PubMed:34644293).
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain, motif, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 7-303 | Helicase ATP-binding | ||||
Sequence: AGIPVHFPFEPYPVQRAYMEKVIHCLRDGTNGVLESPTGTGKTLSLLCSSLAWIRTRQSEHQKQMVKMEKADFSGLGGGAPGGDLSELAKTMGRANNWGVPKVIYASRTHSQLTQAMRELKRTAYANMRSVVLGSRDQLCIHPEVMREQGNSNKTNMCKLRVHSKTCSFQMRVESRKDHPDLRGPTIMDIEDLVKVGQRLKICPYFASRELVPQADITFMPYNYLLDPKARKANKIELGNTIVILDEAHNIEKICEESASVQIKSSDVAMAIEDVTHIMQVFASGESQDMAGDEPKD | ||||||
Motif | 252-255 | DEAH box | ||||
Sequence: DEAH | ||||||
Region | 863-883 | Disordered | ||||
Sequence: VKIHKRERSSPTAPESSSQVT |
Sequence similarities
Belongs to the helicase family. RAD3/XPD subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length985
- Mass (Da)109,683
- Last updated2000-05-01 v1
- Checksum84CFDDC26EF255DC
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
M9PGH6 | M9PGH6_DROME | Rtel1 | 985 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE014298 EMBL· GenBank· DDBJ | AAF46074.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT021290 EMBL· GenBank· DDBJ | AAX33438.1 EMBL· GenBank· DDBJ | mRNA | ||
EU217688 EMBL· GenBank· DDBJ | ABW92607.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EU217689 EMBL· GenBank· DDBJ | ABW92608.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EU217690 EMBL· GenBank· DDBJ | ABW92609.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EU217691 EMBL· GenBank· DDBJ | ABW92610.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EU217692 EMBL· GenBank· DDBJ | ABW92611.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EU217693 EMBL· GenBank· DDBJ | ABW92612.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EU217694 EMBL· GenBank· DDBJ | ABW92613.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EU217695 EMBL· GenBank· DDBJ | ABW92614.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EU217696 EMBL· GenBank· DDBJ | ABW92615.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EU217697 EMBL· GenBank· DDBJ | ABW92616.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EU217698 EMBL· GenBank· DDBJ | ABW92617.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EU217699 EMBL· GenBank· DDBJ | ABW92618.1 EMBL· GenBank· DDBJ | Genomic DNA |