Q9W2E7 · RAE1_DROME

Function

function

Probable component of the nuclear pore complex (NPC) which regulates the nuclear export of specific mRNAs and promotes cell cycle progression during mitosis and male meiosis (PubMed:14729268, PubMed:23788425, PubMed:27494403).
Acts with Nup98-96 to promote the nuclear export of specific mRNAs such as Moe, however it does not appear to be required for general nuclear mRNA transport (PubMed:14729268, PubMed:28554770).
Essential mitotic and male meiotic cell cycle regulator with roles in many aspects of the cell cycle including chromatin organization and condensation, spindle assembly, chromosome segregation, and maintaining nuclear structure (PubMed:14729268, PubMed:23788425, PubMed:27494403).
During male meiosis it is required for completion of meiosis I, as well as accurate cytokinesis of the secondary spermatocytes, and postmeiotic differentiation of spermatids (PubMed:23788425).
Acts as a downstream regulatory target of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway to promote mitotic cell cycle progression and proliferation during wing and eye development, and thereby plays a key role in integrating the regulation of proliferation with organ size control (PubMed:14729268, PubMed:27494403).
When the Hippo/SWH signaling pathway is inactive, Rae1 acts independently of yki to increase organ size by promoting mitotic S-phase entry and increase cellular proliferation (PubMed:27494403).
When the Hippo/SWH signaling pathway is active it inhibits the activity of Rae1 in a Wts-dependent manner to restrict organ growth (PubMed:27494403).
However, Rae1 is also able to negatively regulate the levels and activity of yki likely by activating the core kinases of the Hippo/SWH signaling pathway hpo and Wts and increasing the protein levels of hpo, Mer and Wts; it is therefore likely that it functions as part of a negative feedback loop with the Hippo/SWH signaling pathway to regulate pathway homeostasis and prevent organ overgrowth (PubMed:27494403).
Promotes mitotic cell cycle progression, at least in part, by increasing the accumulation of mitotic cyclins such as CycB, possibly by directly up-regulating cyclin transcripts or by inhibiting the anaphase promoting complex/cyclosome (APC/C) activator fzy (PubMed:27494403).
Also required in presynaptic, postmitotic motor neurons to restrain synaptic terminal growth (PubMed:21874015).
Promotes the expression and stability of the an E3 ubiquitin ligase of hiw, and is likely to function in the regulation of synaptic growth by binding to hiw and protecting it from autophagy-mediated degradation (PubMed:21874015).

GO annotations

AspectTerm
Cellular Componentcell periphery
Cellular Componentchromosome
Cellular Componentcytoplasm
Cellular ComponentNebenkern
Cellular Componentnuclear envelope
Cellular Componentnuclear pore
Cellular Componentnucleus
Cellular Componentperinuclear region of cytoplasm
Cellular Componentsperm flagellum
Molecular FunctionRNA binding
Molecular Functionubiquitin binding
Biological Processcell differentiation
Biological Processcell division
Biological Processhippo signaling
Biological Processmale meiosis I
Biological Processmitotic cell cycle
Biological Processnegative regulation of synaptic assembly at neuromuscular junction
Biological Processpositive regulation of G1/S transition of mitotic cell cycle
Biological Processpositive regulation of gene expression
Biological Processpositive regulation of hippo signaling
Biological Processregulation of autophagy
Biological Processregulation of cell population proliferation
Biological Processregulation of developmental growth
Biological Processregulation of G1/S transition of mitotic cell cycle
Biological Processregulation of hippo signaling
Biological ProcessRNA export from nucleus
Biological Processspermatogenesis
Biological Processtranscription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Protein Rae1

Gene names

    • Name
      Rae1
    • ORF names
      CG9862

Organism names

  • Taxonomic identifier
  • Strain
    • Berkeley
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora

Accessions

  • Primary accession
    Q9W2E7
  • Secondary accessions
    • Q7JVX4

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Nucleus envelope
Chromosome
Cytoplasm
Note: Dynamic pattern of localization during the spermatocyte cell cycle. Perinuclear in young primary spermatocytes. In late meiotic prophase spermatocytes, localizes to the nucleus where it co-localizes with three major chromatin clumps, and is also associated with puncta in the cytoplasm. In anaphase I and telophase I, occurs at segregating chromatin and mitochondria localized between the two daughter nuclei. In post-meiotic onion stage spermatids, mainly associates with the Nebenkern (a mitochondrial formation in the sperm) but is not detected at the nucleus.

Keywords

Phenotypes & Variants

Disruption phenotype

Larval lethal (PubMed:27494403).
Larvae die at the third-instar stage (PubMed:27494403).
RNAi-mediated knockdown in the testes results in male sterility likely due to defects in primary spermatocyte nuclear integrity, meiotic chromosome condensation, segregation, and spindle morphology (PubMed:23788425).
These defects lead to a failure to complete meiosis but several aspects of spermatid differentiation can still proceed, including axoneme formation and elongation (PubMed:23788425).
RNAi-mediated knockdown in the developing wing or in the proliferating cells of the developing eye, reduces their organ size (PubMed:27494403).
RNAi-mediated knockdown in larval neuroblasts results in chromatin undercondensation in metaphase chromosomes (PubMed:23788425).
RNAi-mediated knockdown in differentiating eye cells does not result in an obvious phenotype (PubMed:27494403).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis129Male sterility. Spermatocytes display various defects during early meiotic stages, post-meiotic stages and late spermatogenesis. Defects in early meiotic stages result in karyokinesis and cytokinesis abnormailities and a failure to complete meiosis I. Differentiation arrest prior to spermatid individualization results in seminal vesicles lacking mature sperm.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004500941-346Protein Rae1

Proteomic databases

Expression

Tissue specificity

Head (at protein level).

Developmental stage

Larval brain (at protein level).

Gene expression databases

    • FBgn0034646Expressed in secondary oocyte and 22 other cell types or tissues

Interaction

Subunit

Interacts with hiw; the interaction with Rae1 may protect hiw from autophagy-mediated degradation (PubMed:21874015).
Interacts with Nup98-96 (PubMed:21874015, PubMed:28554770).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for repeat.

TypeIDPosition(s)Description
Repeat17-61WD 1
Repeat64-105WD 2
Repeat126-148WD 3
Repeat255-289WD 4

Sequence similarities

Belongs to the WD repeat rae1 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    346
  • Mass (Da)
    38,618
  • Last updated
    2000-05-01 v1
  • Checksum
    17E4650B83E692A2
MFGATQSTNRMNDFEVASPPDDSVSALEFSPSTVQKNFLVAGSWDSTVRCWEVEQNGATVPKSMKTMGGPVLDVCWSDDGSKVFVASCDKQVKLWDLASDQVMQVAAHDGPVKTCHMVKGPTYTCLMTGSWDKTLKFWDTRSPNPMMTINLPERCYCADVEYPMAVVGTANRGLIIYSLQNSPTEYKRQESPLKYQHRAISIFRDKKKEPTGCALGSIEGRVAIQYVNPGNPKDNFTFKCHRTTGTSGYQDIYAVNDIAFHPVHGTLVTVGSDGTFSFWDKDARTKLKSSETMDQSITKCGFNANGQIFAYAVGYDWSKGHEYFNPAKKPQIFLRSCYDELKPRIN

Sequence caution

The sequence AAM49937.2 differs from that shown. Reason: Erroneous initiation Extended N-terminus.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE013599
EMBL· GenBank· DDBJ
AAF46745.1
EMBL· GenBank· DDBJ
Genomic DNA
AY118568
EMBL· GenBank· DDBJ
AAM49937.2
EMBL· GenBank· DDBJ
mRNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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