Q9VVI3 · NEDD4_DROME
- ProteinE3 ubiquitin-protein ligase Nedd-4
- GeneNedd4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1007 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Essential E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Down-regulates Notch signaling pathway by promoting N/Notch ubiquitination, endocytosis and degradation. During development suppresses activation of the Notch signaling pathway in imaginal disk cells (PubMed:17084358).
Catalytic activity
Pathway
Protein modification; protein ubiquitination.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 974 | Glycyl thioester intermediate | ||||
Sequence: C |
GO annotations
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameE3 ubiquitin-protein ligase Nedd-4
- EC number
- Short namesDNedd4
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ9VVI3
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 974 | Abolishes activity; no effect on interaction with comm. | ||||
Sequence: C → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000120322 | 1-1007 | E3 ubiquitin-protein ligase Nedd-4 | |||
Sequence: MSARSSGLVAAAALPVPSSSSSVAGGDVPRPPPRRRAASVAGQQQTRQEFGNGYTPRRSLAAVNDSGDSCHLRIVVLTGQSLAKKDIFGASDPYVRIDLNTINGDINIDSVLTKTKKKTLNPTWNEEFIFRVKPSEHKLVFQVFDENRLTRDDFLGMVELTLVNLPTEQEGRTIGEQSYTLRPRRSVGAKSRIKGTLRIYHAFIRETREQSEPSSGNSDGEWEHVEATNAGETSAQPHPFPTGGHDALPAGWEERQDANGRTYYVNHTARTTQWDRPTVLNSHSSQSTDDQLASDFQRRFHISVDDTESGRSADSISHNSIEDNNNAAGLAYTPKTAATSSAPPNTPTNNNGILAQIAMQYRAEEDQDPTVDHTSFVYNSLRHPVAHRQPEISATSLQNDLRPVREAPGVPDIAITNPFTRRAAGNMAGGAGWQQERRRQQMQLHIQQHQQRQQQQQQNRILLDVDHRQQEPQHRGQRHQQQHRPSNEDTDHTDSHNPSDISAPSTRRNSEEDNAAVPPMEQNTGGEEEPLPPRWSMQVAPNGRTFFIDHASRRTTWIDPRNGRASPMPNQTRRVEDDLGPLPEGWEERVHTDGRVFYIDHNTRTTQWEDPRLSNPNIAGQAVPYSRDYKQKYEYFKSHIRKPTNVPNKFEIRIRRTSILEDSYRIISSVTKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKENDPRILELTFCLDEDVFGQKSQHELKPGGANIDVTNENKDEYIKLVIEWRFVARVKEQMSSFLDGFGSIIPLNLIKIFDEHELELLMCGIQNIDVKDWRENTLYKGDYHMNHIIIQWFWRAVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQMFTIEKWGTPNNFPRAHTCFNRLDLPPYEGYLQLKDKLIKAIEGSQGFAGVD |
Proteomic databases
Expression
Interaction
Subunit
Interacts (via WW2 domain) with comm (via PY-motifs) (PubMed:12165468, PubMed:16531238).
Interacts with N (PubMed:15620649).
Interacts with N (PubMed:15620649).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9VVI3 | comm Q24139 | 3 | EBI-498113, EBI-118294 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 15-58 | Disordered | ||||
Sequence: PVPSSSSSVAGGDVPRPPPRRRAASVAGQQQTRQEFGNGYTPRR | ||||||
Compositional bias | 39-53 | Polar residues | ||||
Sequence: SVAGQQQTRQEFGNG | ||||||
Domain | 52-175 | C2 | ||||
Sequence: NGYTPRRSLAAVNDSGDSCHLRIVVLTGQSLAKKDIFGASDPYVRIDLNTINGDINIDSVLTKTKKKTLNPTWNEEFIFRVKPSEHKLVFQVFDENRLTRDDFLGMVELTLVNLPTEQEGRTIG | ||||||
Domain | 246-279 | WW 1 | ||||
Sequence: DALPAGWEERQDANGRTYYVNHTARTTQWDRPTV | ||||||
Region | 303-327 | Disordered | ||||
Sequence: SVDDTESGRSADSISHNSIEDNNNA | ||||||
Compositional bias | 313-327 | Polar residues | ||||
Sequence: ADSISHNSIEDNNNA | ||||||
Region | 394-539 | Disordered | ||||
Sequence: ATSLQNDLRPVREAPGVPDIAITNPFTRRAAGNMAGGAGWQQERRRQQMQLHIQQHQQRQQQQQQNRILLDVDHRQQEPQHRGQRHQQQHRPSNEDTDHTDSHNPSDISAPSTRRNSEEDNAAVPPMEQNTGGEEEPLPPRWSMQV | ||||||
Compositional bias | 436-463 | Polar residues | ||||
Sequence: ERRRQQMQLHIQQHQQRQQQQQQNRILL | ||||||
Compositional bias | 464-496 | Basic and acidic residues | ||||
Sequence: DVDHRQQEPQHRGQRHQQQHRPSNEDTDHTDSH | ||||||
Compositional bias | 497-512 | Polar residues | ||||
Sequence: NPSDISAPSTRRNSEE | ||||||
Domain | 529-562 | WW 2 | ||||
Sequence: EPLPPRWSMQVAPNGRTFFIDHASRRTTWIDPRN | ||||||
Region | 558-586 | Disordered | ||||
Sequence: IDPRNGRASPMPNQTRRVEDDLGPLPEGW | ||||||
Domain | 580-613 | WW 3 | ||||
Sequence: GPLPEGWEERVHTDGRVFYIDHNTRTTQWEDPRL | ||||||
Domain | 672-1006 | HECT | ||||
Sequence: KTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKENDPRILELTFCLDEDVFGQKSQHELKPGGANIDVTNENKDEYIKLVIEWRFVARVKEQMSSFLDGFGSIIPLNLIKIFDEHELELLMCGIQNIDVKDWRENTLYKGDYHMNHIIIQWFWRAVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQMFTIEKWGTPNNFPRAHTCFNRLDLPPYEGYLQLKDKLIKAIEGSQGFAGV |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 4 isoforms produced by Alternative splicing.
Q9VVI3-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length1,007
- Mass (Da)114,875
- Last updated2002-10-01 v2
- ChecksumDC5994BD19543760
Q9VVI3-2
- Name2
Q9VVI3-3
- Name3
- Differences from canonical
- 314-486: Missing
Q9VVI3-4
- Name4
Computationally mapped potential isoform sequences
There are 5 potential isoforms mapped to this entry
Sequence caution
Features
Showing features for alternative sequence, compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_015440 | 1-38 | in isoform 2 and isoform 4 | |||
Sequence: Missing | ||||||
Compositional bias | 39-53 | Polar residues | ||||
Sequence: SVAGQQQTRQEFGNG | ||||||
Alternative sequence | VSP_015441 | 39-63 | in isoform 2 and isoform 4 | |||
Sequence: SVAGQQQTRQEFGNGYTPRRSLAAV → MAESTTTSPSVTSEDGQIHGCNNSD | ||||||
Sequence conflict | 185-188 | in Ref. 2; AAS64973 | ||||
Sequence: RSVG → S | ||||||
Compositional bias | 313-327 | Polar residues | ||||
Sequence: ADSISHNSIEDNNNA | ||||||
Alternative sequence | VSP_015442 | 314-486 | in isoform 3 and isoform 4 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_015443 | 375-387 | in isoform 2 | |||
Sequence: Missing | ||||||
Compositional bias | 436-463 | Polar residues | ||||
Sequence: ERRRQQMQLHIQQHQQRQQQQQQNRILL | ||||||
Compositional bias | 464-496 | Basic and acidic residues | ||||
Sequence: DVDHRQQEPQHRGQRHQQQHRPSNEDTDHTDSH | ||||||
Compositional bias | 497-512 | Polar residues | ||||
Sequence: NPSDISAPSTRRNSEE |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ278468 EMBL· GenBank· DDBJ | CAC42101.1 EMBL· GenBank· DDBJ | mRNA | ||
AE014296 EMBL· GenBank· DDBJ | AAF49328.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014296 EMBL· GenBank· DDBJ | AAS64973.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014296 EMBL· GenBank· DDBJ | AAN11694.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014296 EMBL· GenBank· DDBJ | AAN11695.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY061595 EMBL· GenBank· DDBJ | AAL29143.1 EMBL· GenBank· DDBJ | mRNA | ||
AY058619 EMBL· GenBank· DDBJ | AAL13848.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |