Q9VUT6 · GALT8_DROME

Function

function

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. It can both act as a peptide transferase that transfers GalNAc onto unmodified peptide substrates, and as a glycopeptide transferase that requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Prefers both EA2 and the diglycosylated Muc5AC-3/13 as substrates, albeit at very low levels fro Muc5AC-3/13.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Pathway

Protein modification; protein glycosylation.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site168substrate
Binding site220Mn2+ (UniProtKB | ChEBI)
Binding site221substrate
Binding site222Mn2+ (UniProtKB | ChEBI)
Binding site322substrate
Binding site350Mn2+ (UniProtKB | ChEBI)
Binding site353substrate

GO annotations

AspectTerm
Cellular ComponentGolgi apparatus
Cellular ComponentGolgi membrane
Cellular ComponentGolgi stack
Molecular Functioncarbohydrate binding
Molecular Functionmetal ion binding
Molecular Functionpolypeptide N-acetylgalactosaminyltransferase activity
Biological Processoligosaccharide biosynthetic process
Biological Processprotein O-linked glycosylation

Keywords

Enzyme and pathway databases

Protein family/group databases

    • CBM13Carbohydrate-Binding Module Family 13
    • GT27Glycosyltransferase Family 27

Names & Taxonomy

Protein names

  • Recommended name
    Polypeptide N-acetylgalactosaminyltransferase 8
  • EC number
  • Short names
    pp-GaNTase 8
  • Alternative names
    • Protein-UDP acetylgalactosaminyltransferase 8
    • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 8

Gene names

    • Name
      Pgant8
    • ORF names
      CG7297

Organism names

  • Taxonomic identifier
  • Strain
    • Berkeley
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora

Accessions

  • Primary accession
    Q9VUT6

Proteomes

Organism-specific databases

Subcellular Location

Golgi apparatus membrane
; Single-pass type II membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-11Cytoplasmic
Transmembrane12-31Helical; Signal-anchor for type II membrane protein
Topological domain32-590Lumenal

Keywords

PTM/Processing

Features

Showing features for chain, glycosylation, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00000591621-590Polypeptide N-acetylgalactosaminyltransferase 8
Glycosylation77N-linked (GlcNAc...) asparagine
Disulfide bond117↔345
Glycosylation241N-linked (GlcNAc...) asparagine
Disulfide bond336↔419
Disulfide bond459↔475
Disulfide bond502↔517
Disulfide bond546↔561

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in developing oocytes and egg chambers. During embryonic stages 9-11, expressed in the primordium of the foregut, midgut and hindgut. During embryonic stages 12-13, expressed in the posterior midgut and hindgut. During embryonic stages 14-15, expression continues in the hindgut. No expression detected during embryonic stages 16-17 or in third instar larvae imaginal disks.

Developmental stage

Expressed both maternally and zygotically. Weakly expressed during early embryonic stages but increases during 12-24 hours of embryogenesis through larval development and continues to be expressed throughout adulthood, albeit at slightly lower levels in males than females.

Gene expression databases

    • FBgn0036529Expressed in embryonic/larval hemocyte (Drosophila) and 20 other cell types or tissues

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region127-236Catalytic subdomain A
Region291-353Catalytic subdomain B
Domain446-573Ricin B-type lectin

Domain

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    590
  • Mass (Da)
    68,100
  • Last updated
    2000-05-01 v1
  • Checksum
    B18B759A9FC2B021
MCLDIWRHKKKVLPLLLLMAIGSIIYYLYTLKLEGERDESATSTTSRLERDIRDLQAVFESEVIPDLGALGRPARGNWTEEQLEAIAKSQRETGYNAWLSKRISPERSLYDMRHRSCKKLKYPMEKLPSVSVVITYHNEEASVLLRTLSSLRSRTPIQLLREVILVDDGSTQADEKLNDFIKIKFLNMVQHRRITTQVGLMHARVVGAELALADVLVFLDSHVEVTKGWLEPLIAPILEDNRTCTTPIIDTIDFDNFAYRRGKPSRGFFNWEFNYIQLPLLKEEAVAMPAPHKNPIMNGGLFAIGREWFSELGGYDKGLKIWGAEQFELSLKLWLCGGQILEVPCSRVGHLFRDGNFQIRYTNKDKNSEKKLISRNYRRVAEIWLDEYKDKLFANMPHLTVIPVGNLAEQRDLKNRLHCKPFKWFLDNLATDFLNLYPILDPAEYASGVLQSISSPKLCLDRKDPSHGQPKLAPCSSDHVFPSPEQYWSLTNHRELRSGFYCLEVRNHGVNVHIYQCHGQSGNQFWSFDSKTHQVISGQQQNFRHCLEAQPELNAVTSSVCDPKNHKQQWKFGYLNSQRLQHFWDNVKTQ

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE014296
EMBL· GenBank· DDBJ
AAF49587.1
EMBL· GenBank· DDBJ
Genomic DNA
AY070966
EMBL· GenBank· DDBJ
AAL48588.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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