Q9VTH0 · PLOD_DROME
- ProteinProcollagen-lysine,2-oxoglutarate 5-dioxygenase
- GenePlod
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids721 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Forms hydroxylysine residues in collagen type IV (By similarity).
Required for the secretion of collagen type IV (vkg) from haemocytes, fat body and follicle cells (PubMed:20888931, PubMed:23369713).
Required for the secretion of collagen type IV (vkg) from haemocytes, fat body and follicle cells (PubMed:20888931, PubMed:23369713).
Catalytic activity
- 2-oxoglutarate + L-lysyl-[collagen] + O2 = (5R)-5-hydroxy-L-lysyl-[collagen] + CO2 + succinate
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Fe2+ ion per subunit.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | extracellular space | |
Cellular Component | perinuclear region of cytoplasm | |
Molecular Function | iron ion binding | |
Molecular Function | L-ascorbic acid binding | |
Molecular Function | procollagen galactosyltransferase activity | |
Molecular Function | procollagen glucosyltransferase activity | |
Molecular Function | procollagen-lysine 5-dioxygenase activity | |
Biological Process | growth of a germarium-derived egg chamber |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProcollagen-lysine,2-oxoglutarate 5-dioxygenase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ9VTH0
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Detected in the ER of fat body cells and haemocytes. Also detected in small puncta in the extracellular space, particularly in the region surrounding the haemocytes.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
RNAi-mediated knockdown in follicle cells results in round eggs. Collagen type IV protein vkg accumulates in the basal cytoplasm of the follicle cells and is likely to cause the distended ER region in the basal cytoplasm.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 75 | Forms round eggs. Intracellular vkg accumulates at the basal surface of follicle cells, and vkg levels are reduced in the basal membrane. | ||||
Sequence: G → D |
PTM/Processing
Features
Showing features for signal, chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-21 | |||||
Sequence: MRIQQSALLLLLLAVTSQGDA | ||||||
Chain | PRO_5015100590 | 22-721 | Procollagen-lysine,2-oxoglutarate 5-dioxygenase | |||
Sequence: ESNWNDKIKVFTVATEPTDGYTRYIRSARVYDIEVTTLGLGEEWKGGDMQKPGGGFKLNLLREAIAPYKNEPETIILFTDSYDVIITTTLDEIFEKFKESGAKILFSAEKYCWPDKSLANDYPEVEGKASRFLNSGAFIGYAPQVFALLVDPIEDTADDQLYFTKIFLDETKRAKLGLKLDVQSRLFQNLHGAKNDVKLKVDLESNQGVLQNVDFMTTPSIIHGNGLSKVDLNAYGNYLARTFNGVCLLCQENLLDLEETNLPVISLALMVTQPVPFFDQFLEGIESLNYPKEKLHLLIYSNVAFHDDDIKSFVNKHAKEYATAKFALSTDELDERQGRQLALDKARLHQSDYIFFVDADAHIDDGEVLRELLRLNKQFVAPIFSKHKELWSNFWGALSEGGYYARSHDYVDIVKRELIGMFNVPHVTSIYLVKKTAFDAISFKHKEFDPDMAMCESLRNAGIFMYASNLRIFGHLVNADDFNTTVTRPDFYTLFSNEIDWTEKYIHPNYSLQLNESNKIQQPCPDVYWFQIVSDAFCDDLVAIMEAHNGWSDGSNNDNRLEGGYEAVPTRDIHMKQVGLERLYLKFLQMFVRPLQERAFTGYFHNPPRALMNFMVRYRPDEQPSLRPHHDSSTYTINIAMNRAGIDYQGGGCRFIRYNCSVTDTKKGWMLMHPGRLTHYHEGLLVTNGTRYIMISFIDP | ||||||
Glycosylation | 504 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 530 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 536 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 680 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 709 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Developmental stage
Expressed throughout embryogenesis (at protein level). First detected at stage 12 in the haemocytes and in the fat body at stage 13 (at protein level). High levels also detected in the anal pads of stage 16 embryos (at protein level).
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 627-721 | Fe2OG dioxygenase | ||||
Sequence: NPPRALMNFMVRYRPDEQPSLRPHHDSSTYTINIAMNRAGIDYQGGGCRFIRYNCSVTDTKKGWMLMHPGRLTHYHEGLLVTNGTRYIMISFIDP |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length721
- Mass (Da)82,510
- Last updated2000-05-01 v1
- Checksum01208211B39C9E5A
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 141 | in Ref. 3; AAO41443 | ||||
Sequence: N → D |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE014296 EMBL· GenBank· DDBJ | AAF50079.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014296 EMBL· GenBank· DDBJ | AAN11883.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT003764 EMBL· GenBank· DDBJ | AAO41443.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AY118559 EMBL· GenBank· DDBJ | AAM49928.1 EMBL· GenBank· DDBJ | mRNA |