Q9VRC9 · NPC1B_DROME
- ProteinNPC intracellular cholesterol transporter 1 homolog 1b
- GeneNpc1b
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1254 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Important for cholesterol absorption at the midgut epithelium. Acts only in the early steps of sterol absorption, prior to Npc1a-dependent intracellular sterol trafficking.
Catalytic activity
- cholesterol(in) = cholesterol(out)
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Molecular Function | cholesterol binding | |
Molecular Function | lipid transporter activity | |
Biological Process | central nervous system development | |
Biological Process | cholesterol homeostasis | |
Biological Process | cholesterol metabolic process | |
Biological Process | cholesterol transport | |
Biological Process | dorsal closure | |
Biological Process | intestinal cholesterol absorption | |
Biological Process | peripheral nervous system development |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNPC intracellular cholesterol transporter 1 homolog 1b
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ9VRC9
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 17-272 | Extracellular | ||||
Sequence: QSAEQLGCIWYGQSHMIGAHWVNKGDTNPARPLNSPTSEAIFAKRCPMLYKEYKGESGEDELSLCCDAAQIETMESGLSQADGVFSRCPTCTRNMALTVCAMTCAKNHTLFLTAYNDTNDAGVDYVKYIDYRLTDDTVSKIYNSCIGIQHTQTGRPAMDLGCGSYNAKTCNYRRWYEFMGDVSGDYVPFQINYKWSEDAEEGSNEIYLDLSPLKCGESYEDSYACACIDCEESCPLTDAPTGPDELWKIAGLYGVT | ||||||
Transmembrane | 273-293 | Helical | ||||
Sequence: FILALIIACALSFFIFWGAFG | ||||||
Topological domain | 294-325 | Cytoplasmic | ||||
Sequence: KTSAPSVCMPTLFGEFFYHGFRIWGTFCAKHP | ||||||
Transmembrane | 326-346 | Helical | ||||
Sequence: VIVLALCSWAIAGLSFGIRYM | ||||||
Topological domain | 347-593 | Extracellular | ||||
Sequence: TITTDPVELWAGEESQTRIEKDYFDQHFGPFYRTNQMFVKAVNQTYFTHETSNGVLNFGPAFEYNFLKEVFELQDSIMKLGMADNEGLDKICYAPVLMAGETPTVDRCAIQSVYGYFQHDMDRFENSYVDSNNYTINYLNQLEDCLRVPMMEDCFGTFGGPIEPGIAVGGMPKVAVGEDPDYMLATGLVLTFLGRNYNDESKLEPNMKWEKLFVDFLRDYKSDRLDIAYMAERSIQDAIVELSEGEV | ||||||
Transmembrane | 594-614 | Helical | ||||
Sequence: STVVISYVVMFVYVAIALGHI | ||||||
Topological domain | 615-625 | Cytoplasmic | ||||
Sequence: RSCRGFLRESR | ||||||
Transmembrane | 626-646 | Helical | ||||
Sequence: IMLAIGGIVIVLASVVCSLGF | ||||||
Topological domain | 647-657 | Extracellular | ||||
Sequence: WGYLDVTTTML | ||||||
Transmembrane | 658-678 | Helical | ||||
Sequence: AIEVIPFLVLAVGVDNIFIMV | ||||||
Topological domain | 679-736 | Cytoplasmic | ||||
Sequence: HTYQRLDHSKFKTTHEAIGEAIGQVGPSILQTAGSEMACFAIGCISDMPAVKTFAMYA | ||||||
Transmembrane | 737-757 | Helical | ||||
Sequence: AIAILLDFLLQITAFVALMAI | ||||||
Topological domain | 758-815 | Extracellular | ||||
Sequence: DEKRYLDGRLDMLCCVKSGGKKINDEDGDGVDRPKEVGLLETLFKNFYSPFLLSKPVK | ||||||
Transmembrane | 816-836 | Helical | ||||
Sequence: VSVLLIFTVITCLSLMVTPSI | ||||||
Topological domain | 837-857 | Cytoplasmic | ||||
Sequence: EKGLDQEMSMPKNSHVVKYFR | ||||||
Transmembrane | 858-878 | Helical | ||||
Sequence: YMVDLLAMGAPVYWVLKPGLN | ||||||
Topological domain | 879-1079 | Extracellular | ||||
Sequence: YSEPLQQNLICGGVECNNNSLSVQLYTQAQYPEITSLARPASSWLDDYIDWLAISDCCKYNVTTGGFCSSNSKSEDCLPCERGFTENGLRPDAETFNKYIPYFLFDLPDAECAKAGRASYADAVIYTIDDVGMSTVQDSYFMQYSTTSTTSEEFYSQLREVRRISGEINAMFKENNVDAEIFAYCVFYIYYEQYLTIWGDA | ||||||
Transmembrane | 1080-1100 | Helical | ||||
Sequence: MFSLGMSLVAIFLVTLLITGL | ||||||
Topological domain | 1101-1105 | Cytoplasmic | ||||
Sequence: DITST | ||||||
Transmembrane | 1106-1126 | Helical | ||||
Sequence: FIVLFMVICILINMLGMMWAW | ||||||
Topological domain | 1127-1132 | Extracellular | ||||
Sequence: SINLNA | ||||||
Transmembrane | 1133-1153 | Helical | ||||
Sequence: ISLVNLVVCVGIGVEFVAHIV | ||||||
Topological domain | 1154-1174 | Cytoplasmic | ||||
Sequence: RSFKRAEGTAQERARHSLNVT | ||||||
Transmembrane | 1175-1195 | Helical | ||||
Sequence: GSSVLSGITLTKFAGIVVLGF | ||||||
Topological domain | 1196-1207 | Extracellular | ||||
Sequence: SNSQIFQVFYFR | ||||||
Transmembrane | 1208-1228 | Helical | ||||
Sequence: MYLGIVLIGAAHGLILLPVLL | ||||||
Topological domain | 1229-1254 | Cytoplasmic | ||||
Sequence: SLLGPPQKLARSSGAEPTASITITTN |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Larval lethal at the second instar stage. In larvae dietary cholesterol absorption is impaired. In the larval midgut there is a severe reduction in cholesterol and failure to accumulate cholesterol-rich trafficking organelles. No effect on total cholesterol content, possibly due to maternal contributions. Glucose absorption in the midgut is not affected.
PTM/Processing
Features
Showing features for signal, chain, disulfide bond, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-16 | |||||
Sequence: MKVIFATIWLIAGAWS | ||||||
Chain | PRO_5004338412 | 17-1254 | NPC intracellular cholesterol transporter 1 homolog 1b | |||
Sequence: QSAEQLGCIWYGQSHMIGAHWVNKGDTNPARPLNSPTSEAIFAKRCPMLYKEYKGESGEDELSLCCDAAQIETMESGLSQADGVFSRCPTCTRNMALTVCAMTCAKNHTLFLTAYNDTNDAGVDYVKYIDYRLTDDTVSKIYNSCIGIQHTQTGRPAMDLGCGSYNAKTCNYRRWYEFMGDVSGDYVPFQINYKWSEDAEEGSNEIYLDLSPLKCGESYEDSYACACIDCEESCPLTDAPTGPDELWKIAGLYGVTFILALIIACALSFFIFWGAFGKTSAPSVCMPTLFGEFFYHGFRIWGTFCAKHPVIVLALCSWAIAGLSFGIRYMTITTDPVELWAGEESQTRIEKDYFDQHFGPFYRTNQMFVKAVNQTYFTHETSNGVLNFGPAFEYNFLKEVFELQDSIMKLGMADNEGLDKICYAPVLMAGETPTVDRCAIQSVYGYFQHDMDRFENSYVDSNNYTINYLNQLEDCLRVPMMEDCFGTFGGPIEPGIAVGGMPKVAVGEDPDYMLATGLVLTFLGRNYNDESKLEPNMKWEKLFVDFLRDYKSDRLDIAYMAERSIQDAIVELSEGEVSTVVISYVVMFVYVAIALGHIRSCRGFLRESRIMLAIGGIVIVLASVVCSLGFWGYLDVTTTMLAIEVIPFLVLAVGVDNIFIMVHTYQRLDHSKFKTTHEAIGEAIGQVGPSILQTAGSEMACFAIGCISDMPAVKTFAMYAAIAILLDFLLQITAFVALMAIDEKRYLDGRLDMLCCVKSGGKKINDEDGDGVDRPKEVGLLETLFKNFYSPFLLSKPVKVSVLLIFTVITCLSLMVTPSIEKGLDQEMSMPKNSHVVKYFRYMVDLLAMGAPVYWVLKPGLNYSEPLQQNLICGGVECNNNSLSVQLYTQAQYPEITSLARPASSWLDDYIDWLAISDCCKYNVTTGGFCSSNSKSEDCLPCERGFTENGLRPDAETFNKYIPYFLFDLPDAECAKAGRASYADAVIYTIDDVGMSTVQDSYFMQYSTTSTTSEEFYSQLREVRRISGEINAMFKENNVDAEIFAYCVFYIYYEQYLTIWGDAMFSLGMSLVAIFLVTLLITGLDITSTFIVLFMVICILINMLGMMWAWSINLNAISLVNLVVCVGIGVEFVAHIVRSFKRAEGTAQERARHSLNVTGSSVLSGITLTKFAGIVVLGFSNSQIFQVFYFRMYLGIVLIGAAHGLILLPVLLSLLGPPQKLARSSGAEPTASITITTN | ||||||
Disulfide bond | 24↔81 | |||||
Sequence: CIWYGQSHMIGAHWVNKGDTNPARPLNSPTSEAIFAKRCPMLYKEYKGESGEDELSLC | ||||||
Disulfide bond | 62↔116 | |||||
Sequence: CPMLYKEYKGESGEDELSLCCDAAQIETMESGLSQADGVFSRCPTCTRNMALTVC | ||||||
Disulfide bond | 82↔120 | |||||
Sequence: CDAAQIETMESGLSQADGVFSRCPTCTRNMALTVCAMTC | ||||||
Disulfide bond | 104↔241 | |||||
Sequence: CPTCTRNMALTVCAMTCAKNHTLFLTAYNDTNDAGVDYVKYIDYRLTDDTVSKIYNSCIGIQHTQTGRPAMDLGCGSYNAKTCNYRRWYEFMGDVSGDYVPFQINYKWSEDAEEGSNEIYLDLSPLKCGESYEDSYAC | ||||||
Disulfide bond | 107↔161 | |||||
Sequence: CTRNMALTVCAMTCAKNHTLFLTAYNDTNDAGVDYVKYIDYRLTDDTVSKIYNSC | ||||||
Glycosylation | 123 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 132 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 178↔186 | |||||
Sequence: CGSYNAKTC | ||||||
Disulfide bond | 231↔246 | |||||
Sequence: CGESYEDSYACACIDC | ||||||
Disulfide bond | 243↔250 | |||||
Sequence: CIDCEESC | ||||||
Glycosylation | 389 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 438↔454 | |||||
Sequence: CYAPVLMAGETPTVDRC | ||||||
Glycosylation | 479 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 491↔500 | |||||
Sequence: CLRVPMMEDC | ||||||
Disulfide bond | 889↔894 | |||||
Sequence: CGGVEC | ||||||
Glycosylation | 896 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 935↔990 | |||||
Sequence: CCKYNVTTGGFCSSNSKSEDCLPCERGFTENGLRPDAETFNKYIPYFLFDLPDAEC | ||||||
Disulfide bond | 936↔958 | |||||
Sequence: CKYNVTTGGFCSSNSKSEDCLPC | ||||||
Glycosylation | 939 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 946↔955 | |||||
Sequence: CSSNSKSEDC |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the midgut.
Developmental stage
Expressed specifically in the midgut epithelium. In third-instar larvae, expressed in the epithelium of two distinct areas of the midgut; the m2 and m10-m12 compartments. Levels decrease during pupation, and then increase in the adult midgut 2 days after eclosion.
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 592-757 | SSD | ||||
Sequence: EVSTVVISYVVMFVYVAIALGHIRSCRGFLRESRIMLAIGGIVIVLASVVCSLGFWGYLDVTTTMLAIEVIPFLVLAVGVDNIFIMVHTYQRLDHSKFKTTHEAIGEAIGQVGPSILQTAGSEMACFAIGCISDMPAVKTFAMYAAIAILLDFLLQITAFVALMAI |
Sequence similarities
Belongs to the patched family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length1,254
- Mass (Da)139,556
- Last updated2009-03-03 v2
- Checksum01A76C1B1184F249
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE014298 EMBL· GenBank· DDBJ | AAF50873.2 EMBL· GenBank· DDBJ | Genomic DNA |