Q9VQL7 · Q9VQL7_DROME

Function

function

Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.

Catalytic activity

  • (2E)-decenoyl-[ACP] + NADPH + H+ = decanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-dodecenoyl-[ACP] + NADPH + H+ = dodecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-hexadecenoyl-[ACP] + NADPH + H+ = hexadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-hexenoyl-[ACP] + NADPH + H+ = hexanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-octadecenoyl-[ACP] + NADPH + H+ = octadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-octenoyl-[ACP] + NADPH + H+ = octanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-tetradecenoyl-[ACP] + NADPH + H+ = tetradecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • 3-oxobutanoyl-[ACP] + NADPH + H+ = (3R)-hydroxybutanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxodecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxydecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxododecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxydodecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxohexadecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyhexadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxohexanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyhexanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxooctadecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyoctadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxooctanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyoctanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxotetradecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxytetradecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • hexadecanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxooctadecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • hexanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxooctanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • octanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxodecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • tetradecanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxohexadecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • hexadecanoyl-[ACP] + H2O = hexadecanoate + holo-[ACP] + H+
    This reaction proceeds in the forward direction.
    EC:3.1.2.14 (UniProtKB | ENZYME | Rhea)
  • tetradecanoyl-[ACP] + H2O = tetradecanoate + holo-[ACP] + H+
    This reaction proceeds in the forward direction.
    EC:3.1.2.14 (UniProtKB | ENZYME | Rhea)
  • a (3R)-hydroxyacyl-[ACP] + NADP+ = a 3-oxoacyl-[ACP] + NADPH + H+
    This reaction proceeds in the backward direction.
    EC:1.1.1.100 (UniProtKB | ENZYME | Rhea)
  • a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
    EC:4.2.1.59 (UniProtKB | ENZYME | Rhea)
  • a 2,3-saturated acyl-[ACP] + NADP+ = a (2E)-enoyl-[ACP] + NADPH + H+
    This reaction proceeds in the backward direction.
    EC:1.3.1.39 (UniProtKB | ENZYME | Rhea)
  • a fatty acyl-[ACP] + malonyl-[ACP] + H+ = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
    EC:2.3.1.41 (UniProtKB | ENZYME | Rhea)
  • holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA
    This reaction proceeds in the forward direction.
    EC:2.3.1.38 (UniProtKB | ENZYME | Rhea)
  • acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H+ = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+.
    EC:2.3.1.85 (UniProtKB | ENZYME | Rhea)
  • acetyl-[ACP] + malonyl-[ACP] + H+ = 3-oxobutanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • butanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxohexanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • decanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxododecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • dodecanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxotetradecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site911Proton acceptor; for dehydratase activity
Active site1062Proton donor; for dehydratase activity

GO annotations

AspectTerm
Molecular Function(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
Molecular Function3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
Molecular Function3-oxoacyl-[acyl-carrier-protein] synthase activity
Molecular Function[acyl-carrier-protein] S-acetyltransferase activity
Molecular Functionenoyl-[acyl-carrier-protein] reductase (NADPH) activity
Molecular Functionfatty acid synthase activity
Molecular Functionfatty acyl-[ACP] hydrolase activity
Molecular Functionphosphopantetheine binding
Biological Processcellular response to sucrose stimulus
Biological Processfatty acid biosynthetic process
Biological Processresponse to sucrose
Biological Processtriglyceride biosynthetic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

Gene names

    • Name
      FASN1
    • Synonyms
      anon-EST:Posey235
      , anon-EST:Posey43
      , anon-WO0138581.1
      , BcDNA:GH07626
      , BcDNA:gh07626
      , dFAS
      , dFASN
      , dFASN1
      , DM_7295848
      , Dmel\CG3523
      , FAS
      , Fas
      , fas
      , FAS1
      , FAS[CG3523]
      , FASN
      , Fasn
      , fasn
      , Fasn1
      , FASN[CG3523]
      , Fatty acid synthase
    • ORF names
      CG3523
      , Dmel_CG3523

Organism names

  • Taxonomic identifier
  • Strain
    • Berkeley
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora

Accessions

  • Primary accession
    Q9VQL7
  • Secondary accessions
    • Q9Y129

Proteomes

Organism-specific databases

PTM/Processing

Keywords

Expression

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain37-444Ketosynthase family 3 (KS3)
Region879-998N-terminal hotdog fold
Domain879-1145PKS/mFAS DH
Region1013-1145C-terminal hotdog fold
Domain2046-2123Carrier

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,438
  • Mass (Da)
    266,444
  • Last updated
    2000-05-01 v1
  • Checksum
    D336104713119865
MPARFAEEVITAEPAQRAAPQLDLGGGHYVPRQQHLNDEIAITGFSGRLPESSTIEEFKQNLFDGVDMVNDDPRRWERGLYGLPDRIGKLKDSDLENFDQQFFGVHQKQAECMDPLLRMLLELTHEAIIDAGLNPSDLRGSRTGVYIGVSNSETEQHWCSDADRVNGYGLTGCARAMFANRISFTFDFKGPSYSIDTACSSSLYALEQAFSDMREGKVDNALVAGAGLILKPTMSLQFKRLNMLSPDGSCKAFDESGNGYVRSDGCVVLLLQRTSAARRVYASILNVRTNTDGFKEQGITYPIGKMQNRLIRETYEEIGLNPADVVYVEAHGTGTKVGDPQEVNSITDFFCKDRTTPLLIGSVKSNMGHSEPASGVCSVAKILIAMEEGVIPGNLHYNKPNPDLYGLVDGRLKVVDRNLPWNGGIIGLNSFGFGGANAHVILKSNPKPKALTPKDGALKVVLASGRTFEAVEQLLESASTNADDDEYLQLINEIHSKAIPNHFFRGYGVVSSKGTHQREVIESNDDKRPIWYIYSGMGSQWASMAKDLMKIEAFAKTIQRCADVLKPEGVDLIDVLTRSTDKSFENILNSFISIAAMQVALTDLLSSLGIHPDGIVGHSVGELGCAYADGCFTPEQTVLAAYWRGKSILDTQLAKGKMAAVGLSWEDAHSRVPSDCFPVCHNSEDNCTISGPEASIEALVAKLNAEGVFAKAVNSSGYAFHSKYIAEAGPKLRKSLEKIIPNAKNRTARWISTSIPESAWNTPVAKQSSAAYHVNNLLSPVLFHEALQHVPKNAISVEIAPHGLLQAILKRALGPDATNLSLVKRGHENNVEFFLTNVGKLFAAGAQPQVLTLVRPISYPVGRGTPMLNSKVGWDHTQKWLVAKFGKETSSGETIVEVDLSKEDDAFLAGHTIDGRILFPATGYMTLAWQTFAKMQGSEFHKTPVVMENLVFHRATILNKNAVVKFGINFFDGTGAFEICESGSLAVSGKITIPESIDNEELPLEEQTPSAVAKELGTNDVYKELRLRGYDYGGIFRGIVRSDTVASTGKLQWVDNWISFMDTMLQFSILSKNLRELYLPTRIERAVINPAKHFELLSALTKEEQVETGLPVQWYSDINVIKSAGVELRGLKANLAQRRPGTQAPPTLERYQFVPNINTTDLNENSEKARLHALDVAIQVIIENSSGAVKLKGVELANGRNPDVLVANRLLQIIEGEPVLTGDVAVVTSNNNEETITAALGDSGVRVVSKDVLKEPVEQNCHFVFGIDVLSRPDTKTLENSIASIRENGFLILEETLPTYTKTGRALLTKFGFVAVQEQSLGATRVLVLARKAVDLKTRKSVVVVATEQNFNWVDDLKAALATAATEEQYVYVVCQGEELFGAVGLMTCIKNENGGKLARLVFVQDAKAEKFSLTSTLYRQQLEKDLISNVLKNGAWGTFRHLKLETQQATLQVEHAYVNALVKGDLASLKWIEAAQADTAATVDKNLETCTVYYAPINFRDVMLTSGKLAADALPGDLAEQDCVLGLEFAGRDTQGRRVMAMVPAKSLATTCVASKRMMWQIPEKWTMEEASTVPCVYSTVYYALVVRGQMKKGEKILIHAGSGGVGQAAISVALAHGLTVFTTVGSKEKREFLLKRFPKLQERNIGNSRDTSFEQLVLRETKGRGVDLVLNSLSEEKLQASIRCLGLNGRFLEIGKFDLSNNSPLGMSVFLKNTSFHGILLDSVMEGEEEMQNQVVSLVAEGIKTGAVVPLPTSVFNDQQVEQAFRFMASGKHIGKVVIKVRDEEAGKKALQPKPRLINAIPRTYMHPEKSYILVGGLGGFGLELTNWLVTRGARYIVLTSRSGVKTGYQGLMIRRWQERGVKVVIDTSDVTTAAGAKKLLENSNKLALVGGIFNLAAVLRDALIEDQTAKDFKTVADPKVTATKYLDQFSRDICTELDYFICFSSVSCGRGNIGQTNYGLANSAMERICEQRQVSGFPGTAIQWGAIGDTGLVLENLGDNDTVIGGTLPQRMPSCLQTIDLFLQQPHPVVASMVVAEKRKSDQSAGVSLIATIANILGLRDTKNIQDGASLADLGMDSLMSAEIKQTLERNFDIVLSAQEIRQLTFGALKAMDGGADVKPAAAAPAAAAGVPEANITSGGSSRTASPMGDGTQVVFTTSLIPTEAIVQLDTKAPANSKQSPIFFISPIEGFASALEPLAKRLEVPAYGLQYTEAVPSDSLESAAKFFIKQLRTVQPKGPYKLAGYSFGCLLTYVMAGILEETNEVANVIMLDGAPSYVNWYTSSFKQRYTDGTNADNDNQSYGLAYFGIVLANIDYKALVRLLIVIPTWEEKLERFAELMSNEITQPVETIKKSATLFYKKLELADGYQPTLKLKTNVTLVKPTDNSAKLDEDYRLKEVCTKPVEVHTVEGNHRTFLIEDQSLKTIQSILKRLFN

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
B7Z001B7Z001_DROMEFASN12540

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE014134
EMBL· GenBank· DDBJ
AAF51148.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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