Q9VQ89 · MIO_DROME

Function

function

An essential component of the GATOR subcomplex GATOR2 which functions as an activator of the amino acid-sensing branch of the TORC1 signaling pathway (PubMed:23723238, PubMed:27166823).
The two GATOR subcomplexes, GATOR1 and GATOR2, regulate the TORC1 pathway in order to mediate metabolic homeostasis, female gametogenesis and the response to amino acid limitation and complete starvation (PubMed:23723238, PubMed:27166823).
GATOR2 activates the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex, controlling the switch to cell proliferation and growth under nutrient replete conditions and during female oocyte development (PubMed:14973288, PubMed:21521741, PubMed:23723238, PubMed:25512509).
This component is required for activating TORC1 specifically in germline cells to promote cell growth and maintain the oocyte fate (PubMed:21521741, PubMed:23723238, PubMed:27166823).
GATOR1 and GATOR2 act at different stages of oogenesis to regulate TORC1 in order to control meiotic entry and promote oocyte growth and development (PubMed:14973288, PubMed:21521741, PubMed:23723238, PubMed:25512509).
After exactly four mitotic cyst divisions, the GATOR1 complex members (Iml1, Nprl2 and Nprl3) down-regulate TORC1 to slow cellular metabolism and promote the mitotic/meiotic transition (PubMed:25512509).
At later stages of oogenesis, the mio and Nup44A components of the GATOR2 complex inhibit GATOR1 and thus activate TORC1 to promote meiotic progression, and drive oocyte growth and development (PubMed:21521741, PubMed:25512509).
In addition to its role in the regulation of the TORC1 complex, functions independently of TORC1 to prevent the inappropriate accumulation of autolysosomes in germline tissues (PubMed:27166823).

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site741Zn2+ 1 (UniProtKB | ChEBI)
Binding site744Zn2+ 1 (UniProtKB | ChEBI)
Binding site771Zn2+ 1 (UniProtKB | ChEBI)
Binding site774Zn2+ 1 (UniProtKB | ChEBI)
Binding site784Zn2+ 2 (UniProtKB | ChEBI)
Binding site821Zn2+ 3 (UniProtKB | ChEBI)
Binding site824Zn2+ 3 (UniProtKB | ChEBI)
Binding site824Zn2+ 4 (UniProtKB | ChEBI)
Binding site826Zn2+ 4 (UniProtKB | ChEBI)
Binding site829Zn2+ 2 (UniProtKB | ChEBI)
Binding site832Zn2+ 2 (UniProtKB | ChEBI)
Binding site843Zn2+ 4 (UniProtKB | ChEBI)
Binding site848Zn2+ 4 (UniProtKB | ChEBI)
Binding site852Zn2+ 3 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentautolysosome
Cellular Componentcytoplasm
Cellular Componentfemale germ cell nucleus
Cellular ComponentGATOR2 complex
Cellular Componentlysosome
Cellular Componentnucleus
Cellular Componentprotein-containing complex
Cellular ComponentSeh1-associated complex
Molecular Functionmetal ion binding
Biological Processcell division
Biological Processcellular response to amino acid starvation
Biological Processdouble-strand break repair
Biological Processgermarium-derived egg chamber formation
Biological Processmeiotic cell cycle
Biological Processnegative regulation of autophagy
Biological Processoocyte differentiation
Biological Processoocyte fate determination
Biological Processpositive regulation of autophagy
Biological Processpositive regulation of cell size
Biological Processpositive regulation of TOR signaling
Biological Processpositive regulation of TORC1 signaling
Biological Processregulation of meiotic nuclear division

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    GATOR2 complex protein MIOS
  • Alternative names
    • Missing oocyte protein

Gene names

    • Name
      mio
    • ORF names
      CG7074

Organism names

  • Taxonomic identifier
  • Strain
    • Berkeley
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora

Accessions

  • Primary accession
    Q9VQ89
  • Secondary accessions
    • Q95RC2

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Lysosome
Note: Accumulates in pro-oocyte nuclei in early prophase of meiosis I (PubMed:14973288).
In egg chambers, detected in lysosomes and autolysosomes of both fed and starved females (PubMed:25512509).

Keywords

Phenotypes & Variants

Disruption phenotype

Egg chambers contain 16 polyploid nurse cells because the oocyte enters the endocycle and develops as a polyploid nurse cell.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis817-867Significant decrease in TORC1 activity in adult ovaries indicated by reduced phosphorylation of S6K/p70S6K and the accumulation of autolysosomes. Female germline clones are smaller compared to wild-type cells. In contrast, somatic tissues do not display a significant decrease in TORC1 activity and there is only a negligible decrease in cell size. RNAi-mediated knockdown of Nprl1 or Nprl3 rescues the ovarian defects in mutants, but not the accumulation of lysosomes.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003294101-867GATOR2 complex protein MIOS

Proteomic databases

Expression

Tissue specificity

Present in the oocyte.

Gene expression databases

    • FBgn0031399Expressed in adult Malpighian tubule (Drosophila) and 24 other cell types or tissues

Interaction

Subunit

Component of the GATOR complex consisting of mio, Nup44A/Seh1, Im11, Nplr3, Nplr2, Wdr24, Wdr59 and Sec13 (PubMed:27166823).
Within the GATOR complex, probable component of the GATOR2 subcomplex which is likely composed of mio, Nup44A/Seh1, Wdr24, Wdr59 and Sec13 (PubMed:27166823).
Interacts with Wdr24 (PubMed:27166823).
Interacts with nucleoporin Nup44A/Seh1 (PubMed:21521741).

Complex viewer

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for repeat, region, zinc finger.

TypeIDPosition(s)Description
Repeat51-86WD 1
Repeat100-144WD 2
Repeat149-188WD 3
Repeat190-228WD 4
Repeat231-272WD 5
Region350-378Disordered
Zinc finger739-777C4-type
Zinc finger778-857RING-type; atypical

Sequence similarities

Belongs to the WD repeat mio family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    867
  • Mass (Da)
    98,602
  • Last updated
    2002-10-01 v2
  • Checksum
    76AED774B9C34E4D
MSGNTHGLSWFPHFPDKFVSWGQEIHLYEVRRKDDHSQKSRLPYISVNYLANESRYQYARCVAASYHSDQPIIAVGLADGKVGICNFRDTYDSSWEYTPRQQRMCTCLAWNELDANILAIGHDRHRNDTCITIWDIERGVPKETANFFGVGESANSICWDRNHRTVIAGMSQKMIKLFDLRQSNATCQSIQTKTVQGLSVSPNGNYLCSYVDSVITLWDPRNIKSPLRQIQSSKNHLQIAWCPTRTSLLSSLQRDSSYITLYDIRSVDTDNSGEIYHVKRQISPFPARYQHSGKFSFVNCLSWHSRDFERALLLADALNILDFRLPATLHTAHSNRRKLPLLMQRPLYTPASPTSTAATPTQQQPTSSCSTNSGSSLDFSTPGGSPFNVDLLKPELFELDLVDETRQRALEDYGIKPDNKRFGELHLTPYLRNVWSTLNNVYSEDRLTGLKATLGINLGHTSEALMASSRIESQVLQWPEGINNSNKLICYRSEQRDLALQLCGWAFEQELDRFIDQLYANKEYSRAAMICVFHLKIFHACNILSSAADNMRDPSMYRITVIALSSFNADRCSSTWRNQRSSANMQIHDPHLRAVFSFLTMEKDNFDAVLKEEGVSLSDRMAFACKYLSETKLADYVAQQIQAAIDGGDLNGLLLTGESQDGIDILQSYMDTSFDVQTVALVAINYFRQELFEDKRIQYWIASYLDHLNSWGLWEKRAELDIKIESIRPSSRSSRTVFLSCNFCGKSVSNALLDEPRPRSTTTSTNRLSSCPSCRKPLPRCSLCLMHMGTMVNMSNGETPTTTPDVPGWQTKPFSKWFSWCQTCRHGGHTEHIMQWFKQNSECPVSSCNCRCFDMDGTKPNTLRDIS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE014134
EMBL· GenBank· DDBJ
AAF51288.2
EMBL· GenBank· DDBJ
Genomic DNA
AY061485
EMBL· GenBank· DDBJ
AAL29033.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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