Q9VQ89 · MIO_DROME
- ProteinGATOR2 complex protein MIOS
- Genemio
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids867 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
An essential component of the GATOR subcomplex GATOR2 which functions as an activator of the amino acid-sensing branch of the TORC1 signaling pathway (PubMed:23723238, PubMed:27166823).
The two GATOR subcomplexes, GATOR1 and GATOR2, regulate the TORC1 pathway in order to mediate metabolic homeostasis, female gametogenesis and the response to amino acid limitation and complete starvation (PubMed:23723238, PubMed:27166823).
GATOR2 activates the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex, controlling the switch to cell proliferation and growth under nutrient replete conditions and during female oocyte development (PubMed:14973288, PubMed:21521741, PubMed:23723238, PubMed:25512509).
This component is required for activating TORC1 specifically in germline cells to promote cell growth and maintain the oocyte fate (PubMed:21521741, PubMed:23723238, PubMed:27166823).
GATOR1 and GATOR2 act at different stages of oogenesis to regulate TORC1 in order to control meiotic entry and promote oocyte growth and development (PubMed:14973288, PubMed:21521741, PubMed:23723238, PubMed:25512509).
After exactly four mitotic cyst divisions, the GATOR1 complex members (Iml1, Nprl2 and Nprl3) down-regulate TORC1 to slow cellular metabolism and promote the mitotic/meiotic transition (PubMed:25512509).
At later stages of oogenesis, the mio and Nup44A components of the GATOR2 complex inhibit GATOR1 and thus activate TORC1 to promote meiotic progression, and drive oocyte growth and development (PubMed:21521741, PubMed:25512509).
In addition to its role in the regulation of the TORC1 complex, functions independently of TORC1 to prevent the inappropriate accumulation of autolysosomes in germline tissues (PubMed:27166823).
The two GATOR subcomplexes, GATOR1 and GATOR2, regulate the TORC1 pathway in order to mediate metabolic homeostasis, female gametogenesis and the response to amino acid limitation and complete starvation (PubMed:23723238, PubMed:27166823).
GATOR2 activates the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex, controlling the switch to cell proliferation and growth under nutrient replete conditions and during female oocyte development (PubMed:14973288, PubMed:21521741, PubMed:23723238, PubMed:25512509).
This component is required for activating TORC1 specifically in germline cells to promote cell growth and maintain the oocyte fate (PubMed:21521741, PubMed:23723238, PubMed:27166823).
GATOR1 and GATOR2 act at different stages of oogenesis to regulate TORC1 in order to control meiotic entry and promote oocyte growth and development (PubMed:14973288, PubMed:21521741, PubMed:23723238, PubMed:25512509).
After exactly four mitotic cyst divisions, the GATOR1 complex members (Iml1, Nprl2 and Nprl3) down-regulate TORC1 to slow cellular metabolism and promote the mitotic/meiotic transition (PubMed:25512509).
At later stages of oogenesis, the mio and Nup44A components of the GATOR2 complex inhibit GATOR1 and thus activate TORC1 to promote meiotic progression, and drive oocyte growth and development (PubMed:21521741, PubMed:25512509).
In addition to its role in the regulation of the TORC1 complex, functions independently of TORC1 to prevent the inappropriate accumulation of autolysosomes in germline tissues (PubMed:27166823).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 741 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 744 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 771 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 774 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 784 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 821 | Zn2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 824 | Zn2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 824 | Zn2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 826 | Zn2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 829 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 832 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 843 | Zn2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 848 | Zn2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 852 | Zn2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | autolysosome | |
Cellular Component | cytoplasm | |
Cellular Component | female germ cell nucleus | |
Cellular Component | GATOR2 complex | |
Cellular Component | lysosome | |
Cellular Component | nucleus | |
Cellular Component | protein-containing complex | |
Cellular Component | Seh1-associated complex | |
Molecular Function | metal ion binding | |
Biological Process | cell division | |
Biological Process | cellular response to amino acid starvation | |
Biological Process | double-strand break repair | |
Biological Process | germarium-derived egg chamber formation | |
Biological Process | meiotic cell cycle | |
Biological Process | negative regulation of autophagy | |
Biological Process | oocyte differentiation | |
Biological Process | oocyte fate determination | |
Biological Process | positive regulation of autophagy | |
Biological Process | positive regulation of cell size | |
Biological Process | positive regulation of TOR signaling | |
Biological Process | positive regulation of TORC1 signaling | |
Biological Process | regulation of meiotic nuclear division |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameGATOR2 complex protein MIOS
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ9VQ89
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Disruption phenotype
Egg chambers contain 16 polyploid nurse cells because the oocyte enters the endocycle and develops as a polyploid nurse cell.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 817-867 | Significant decrease in TORC1 activity in adult ovaries indicated by reduced phosphorylation of S6K/p70S6K and the accumulation of autolysosomes. Female germline clones are smaller compared to wild-type cells. In contrast, somatic tissues do not display a significant decrease in TORC1 activity and there is only a negligible decrease in cell size. RNAi-mediated knockdown of Nprl1 or Nprl3 rescues the ovarian defects in mutants, but not the accumulation of lysosomes. | ||||
Sequence: Missing |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000329410 | 1-867 | GATOR2 complex protein MIOS | |||
Sequence: MSGNTHGLSWFPHFPDKFVSWGQEIHLYEVRRKDDHSQKSRLPYISVNYLANESRYQYARCVAASYHSDQPIIAVGLADGKVGICNFRDTYDSSWEYTPRQQRMCTCLAWNELDANILAIGHDRHRNDTCITIWDIERGVPKETANFFGVGESANSICWDRNHRTVIAGMSQKMIKLFDLRQSNATCQSIQTKTVQGLSVSPNGNYLCSYVDSVITLWDPRNIKSPLRQIQSSKNHLQIAWCPTRTSLLSSLQRDSSYITLYDIRSVDTDNSGEIYHVKRQISPFPARYQHSGKFSFVNCLSWHSRDFERALLLADALNILDFRLPATLHTAHSNRRKLPLLMQRPLYTPASPTSTAATPTQQQPTSSCSTNSGSSLDFSTPGGSPFNVDLLKPELFELDLVDETRQRALEDYGIKPDNKRFGELHLTPYLRNVWSTLNNVYSEDRLTGLKATLGINLGHTSEALMASSRIESQVLQWPEGINNSNKLICYRSEQRDLALQLCGWAFEQELDRFIDQLYANKEYSRAAMICVFHLKIFHACNILSSAADNMRDPSMYRITVIALSSFNADRCSSTWRNQRSSANMQIHDPHLRAVFSFLTMEKDNFDAVLKEEGVSLSDRMAFACKYLSETKLADYVAQQIQAAIDGGDLNGLLLTGESQDGIDILQSYMDTSFDVQTVALVAINYFRQELFEDKRIQYWIASYLDHLNSWGLWEKRAELDIKIESIRPSSRSSRTVFLSCNFCGKSVSNALLDEPRPRSTTTSTNRLSSCPSCRKPLPRCSLCLMHMGTMVNMSNGETPTTTPDVPGWQTKPFSKWFSWCQTCRHGGHTEHIMQWFKQNSECPVSSCNCRCFDMDGTKPNTLRDIS |
Proteomic databases
Expression
Interaction
Subunit
Component of the GATOR complex consisting of mio, Nup44A/Seh1, Im11, Nplr3, Nplr2, Wdr24, Wdr59 and Sec13 (PubMed:27166823).
Within the GATOR complex, probable component of the GATOR2 subcomplex which is likely composed of mio, Nup44A/Seh1, Wdr24, Wdr59 and Sec13 (PubMed:27166823).
Interacts with Wdr24 (PubMed:27166823).
Interacts with nucleoporin Nup44A/Seh1 (PubMed:21521741).
Within the GATOR complex, probable component of the GATOR2 subcomplex which is likely composed of mio, Nup44A/Seh1, Wdr24, Wdr59 and Sec13 (PubMed:27166823).
Interacts with Wdr24 (PubMed:27166823).
Interacts with nucleoporin Nup44A/Seh1 (PubMed:21521741).
Complex viewer
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for repeat, region, zinc finger.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Repeat | 51-86 | WD 1 | ||||
Sequence: ANESRYQYARCVAASYHSDQPIIAVGLADGKVGICN | ||||||
Repeat | 100-144 | WD 2 | ||||
Sequence: RQQRMCTCLAWNELDANILAIGHDRHRNDTCITIWDIERGVPKET | ||||||
Repeat | 149-188 | WD 3 | ||||
Sequence: GVGESANSICWDRNHRTVIAGMSQKMIKLFDLRQSNATCQ | ||||||
Repeat | 190-228 | WD 4 | ||||
Sequence: IQTKTVQGLSVSPNGNYLCSYVDSVITLWDPRNIKSPLR | ||||||
Repeat | 231-272 | WD 5 | ||||
Sequence: QSSKNHLQIAWCPTRTSLLSSLQRDSSYITLYDIRSVDTDNS | ||||||
Region | 350-378 | Disordered | ||||
Sequence: PASPTSTAATPTQQQPTSSCSTNSGSSLD | ||||||
Zinc finger | 739-777 | C4-type | ||||
Sequence: LSCNFCGKSVSNALLDEPRPRSTTTSTNRLSSCPSCRKP | ||||||
Zinc finger | 778-857 | RING-type; atypical | ||||
Sequence: LPRCSLCLMHMGTMVNMSNGETPTTTPDVPGWQTKPFSKWFSWCQTCRHGGHTEHIMQWFKQNSECPVSSCNCRCFDMDG |
Sequence similarities
Belongs to the WD repeat mio family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length867
- Mass (Da)98,602
- Last updated2002-10-01 v2
- Checksum76AED774B9C34E4D
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE014134 EMBL· GenBank· DDBJ | AAF51288.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY061485 EMBL· GenBank· DDBJ | AAL29033.1 EMBL· GenBank· DDBJ | mRNA |