Q9VQ79 · AIFM1_DROME
- ProteinPutative apoptosis-inducing factor 1, mitochondrial
- GeneAIF
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids739 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Probable NADH oxidoreductase (By similarity).
Mitochondrial effector of cell death that plays roles in developmentally regulated cell death and normal mitochondrial function
Mitochondrial effector of cell death that plays roles in developmentally regulated cell death and normal mitochondrial function
Catalytic activity
- A + H+ + NADH = AH2 + NAD+
Cofactor
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 261-265 | FAD (UniProtKB | ChEBI) | ||||
Sequence: GGGTA | ||||||
Binding site | 295 | FAD (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 300 | FAD (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 358 | FAD (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Binding site | 410 | FAD (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 564 | FAD (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 580-581 | FAD (UniProtKB | ChEBI) | ||||
Sequence: HH |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | mitochondrial intermembrane space | |
Cellular Component | mitochondrion | |
Molecular Function | DNA binding | |
Molecular Function | FAD binding | |
Molecular Function | NAD(P)H oxidase H2O2-forming activity | |
Molecular Function | NADH dehydrogenase activity | |
Molecular Function | protein dimerization activity | |
Biological Process | apoptotic process | |
Biological Process | defense response to fungus | |
Biological Process | mitochondrial ATP synthesis coupled electron transport | |
Biological Process | mitochondrial respiratory chain complex assembly | |
Biological Process | programmed cell death involved in cell development | |
Biological Process | protein import into mitochondrial intermembrane space |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePutative apoptosis-inducing factor 1, mitochondrial
- EC number
- Short namesDmAIF
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ9VQ79
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Loss of zygotic expression results in decreased embryonic cell death and the persistence of differentiated neuronal cells along the ventral nerve cord at late embryonic stages. Embryos that do hatch undergo growth arrest at early larval stages, accompanied by mitochondrial respiratory dysfunction.
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-42 | Mitochondrion | ||||
Sequence: MSIWGVRCLTQRFIRQAYILANRRLLGPVPQRSPPAYAPLRP | ||||||
Chain | PRO_0000022029 | 43-739 | Putative apoptosis-inducing factor 1, mitochondrial | |||
Sequence: AHSSLYQMVKKRTLEARTKLQANKYPNHQVCVTKPSTTPPVEEYETAVEGAGVPAYANAQFQAHSQSEPLFKVGFADVKSVCSAKDVLKSDSAKLSTSQPVLDSCKATSPCEEFKRKRKETTCQPCDEDGTAPGGGDGGDEECECRMKDLRLKCLLGALAALLAGGFLAWFMTRDTDDSEAKKAEAEEEERKRRLVAGLATSPPSSEDLPKHVPYLIIGGGTAAFSAFRAIKSNDATAKVLMISNEFRKPYMRPPLSKELWYTPNPNEDPIKDYRFKQWTGSERSLFFEPDEFFIDPEDLDDNANGGIAVAQGFSVKKVDAQKRIVTLNDGYEISYDECLIATGCAPKNLPMLRDAPPSVLEKVMVYRTPDDFDRLRKLAAEKRSITIVGNGFIGSELACSLAHYSRENNGGKVYQVFQENANMSKVLPNYLSRWTTAKMEAQGVCVIPNASIRSAVRDETNLKLELNNGMTLMSDVVVVCVGCTPNTDLAGPSRLEVDRSLGGFVVNAELEARRNLYVAGDASCFFDPLLGRRRVEHHDHSVVSGRLAGENMTGAKKPYQHQSMFWSDLGPEIGYEGIGLVDSSLPTVGVFALPSESATRVDQLSESSDSDVPETSTSSSQSSKSDAGASQDGVTCDPDEAGNYGKGVIFYLKNDKIVGILLWNLFNRIGLARTIINQNKKYDDLNEVAKLFEIHA |
Proteomic databases
Expression
Developmental stage
Expressed both maternally and zygotically throughout development with slightly lower levels during pupation.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 257-564 | FAD-dependent oxidoreductase | ||||
Sequence: YLIIGGGTAAFSAFRAIKSNDATAKVLMISNEFRKPYMRPPLSKELWYTPNPNEDPIKDYRFKQWTGSERSLFFEPDEFFIDPEDLDDNANGGIAVAQGFSVKKVDAQKRIVTLNDGYEISYDECLIATGCAPKNLPMLRDAPPSVLEKVMVYRTPDDFDRLRKLAAEKRSITIVGNGFIGSELACSLAHYSRENNGGKVYQVFQENANMSKVLPNYLSRWTTAKMEAQGVCVIPNASIRSAVRDETNLKLELNNGMTLMSDVVVVCVGCTPNTDLAGPSRLEVDRSLGGFVVNAELEARRNLYVAGD | ||||||
Compositional bias | 644-673 | Polar residues | ||||
Sequence: VDQLSESSDSDVPETSTSSSQSSKSDAGAS | ||||||
Region | 644-681 | Disordered | ||||
Sequence: VDQLSESSDSDVPETSTSSSQSSKSDAGASQDGVTCDP |
Sequence similarities
Belongs to the FAD-dependent oxidoreductase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 2 isoforms produced by Alternative splicing.
Q9VQ79-2
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameB
- Length739
- Mass (Da)81,217
- Last updated2003-08-15 v2
- Checksum71184B1721AB93AF
Q9VQ79-1
- NameA
- Differences from canonical
- 51-115: Missing
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
M9PBQ4 | M9PBQ4_DROME | AIF | 738 |
Sequence caution
Features
Showing features for alternative sequence, sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_007951 | 51-115 | in isoform A | |||
Sequence: Missing | ||||||
Sequence conflict | 639 | in Ref. 4; AAK93507 | ||||
Sequence: E → D | ||||||
Compositional bias | 644-673 | Polar residues | ||||
Sequence: VDQLSESSDSDVPETSTSSSQSSKSDAGAS |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE014134 EMBL· GenBank· DDBJ | AAF51299.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014134 EMBL· GenBank· DDBJ | AAN10444.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY052083 EMBL· GenBank· DDBJ | AAK93507.1 EMBL· GenBank· DDBJ | mRNA | ||
AY058696 EMBL· GenBank· DDBJ | AAL13925.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
BT044192 EMBL· GenBank· DDBJ | ACH92257.1 EMBL· GenBank· DDBJ | mRNA |