Q9VJK8 · JHAMT_DROME

Function

function

O-methyltransferase that transfers a methyl group from S-adenosyl-L-methionine (SAM) to the carboxyl group of juvenile hormone acids to produce active juvenile hormones in the corpora allata, the last step during juvenile hormone biosynthesis (PubMed:18549957).
Also able to methylate farnesoate to methyl farnesoate (PubMed:18549957).

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
3.32 μMjuvenile hormone III acid
0.18 μMfarnesoate
kcat is 7.7 min-1 for juvenile hormone III acid. kcat is 10.1 min-1 for farnesoate. kcat is 0.103 min-1 for laureate acid. kcat is 0.041 min-1 for palmitate acid.

GO annotations

AspectTerm
Cellular Componentmitochondrion
Molecular Function3,4-dihydroxy-5-polyprenylbenzoic acid O-methyltransferase activity
Molecular Functionfarnesoic acid O-methyltransferase activity
Molecular Functionjuvenile hormone acid methyltransferase activity
Biological Processjuvenile hormone biosynthetic process
Biological Processmale courtship behavior
Biological Processmethylation
Biological Processubiquinone biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Juvenile hormone acid O-methyltransferase
  • EC number
  • Alternative names
    • Juvenile hormone acid methyltransferase
      (DmJHAMT
      )

Gene names

    • Name
      jhamt
    • ORF names
      CG17330

Organism names

  • Taxonomic identifier
  • Strain
    • Berkeley
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora

Accessions

  • Primary accession
    Q9VJK8
  • Secondary accessions
    • Q767F0
    • Q8T900

Proteomes

Organism-specific databases

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004336191-297Juvenile hormone acid O-methyltransferase

Proteomic databases

Expression

Tissue specificity

Predominantly expressed in corpora allata. Also expressed at low level in testis.

Developmental stage

Highly expressed in the first instar larvae and then gradually decreases during larval development. Expression increases again in the wandering third larval stage. The lowest amount of expression is observed in the early and mid-pupal stages. After this, expression increases in the late pupal stage in both male and female adults.

Gene expression databases

    • FBgn0028841Expressed in spermathecum and 14 other cell types or tissues

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the methyltransferase superfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    297
  • Mass (Da)
    34,065
  • Last updated
    2002-10-01 v2
  • Checksum
    9ACFBB7E0472E010
MNQASLYQHANQVQRHDAKLILDEFASTMQWRSDGEDALLDVGSGSGNVLMDFVKPLLPIRGQLVGTDISSQMVHYASKHYQREERTRFQVLDIGCERLPEELSGRFDHVTSFYCLHWVQNLKGALGNIYNLLKPEGGDCLLAFLASNPVYEVYKILKTNDKWSTFMQDVENFISPLHYSLSPGEEFSQLLNDVGFVQHNVEIRNEVFVYEGVRTLKDNVKAICPFLERMPADLHEQFLDDFIDIVISMNLQQGENNEDQKFLSPYKLVVAYARKTPEFVNNVFLEPTHQNLVKGIN

Sequence caution

The sequence AAL68063.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict166in Ref. 1; BAC98836 and 4; AAL68063
Sequence conflict172in Ref. 4; AAL68063
Sequence conflict182in Ref. 1; BAC98836 and 4; AAL68063

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB113579
EMBL· GenBank· DDBJ
BAC98836.1
EMBL· GenBank· DDBJ
mRNA
AE014134
EMBL· GenBank· DDBJ
AAF53533.2
EMBL· GenBank· DDBJ
Genomic DNA
AE014134
EMBL· GenBank· DDBJ
AHN54494.1
EMBL· GenBank· DDBJ
Genomic DNA
AY075194
EMBL· GenBank· DDBJ
AAL68063.1
EMBL· GenBank· DDBJ
mRNA Different initiation

Genome annotation databases

Similar Proteins

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