Q9VJK8 · JHAMT_DROME
- ProteinJuvenile hormone acid O-methyltransferase
- Genejhamt
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids297 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
O-methyltransferase that transfers a methyl group from S-adenosyl-L-methionine (SAM) to the carboxyl group of juvenile hormone acids to produce active juvenile hormones in the corpora allata, the last step during juvenile hormone biosynthesis (PubMed:18549957).
Also able to methylate farnesoate to methyl farnesoate (PubMed:18549957).
Also able to methylate farnesoate to methyl farnesoate (PubMed:18549957).
Catalytic activity
- (2E,6E)-farnesoate + S-adenosyl-L-methionine = methyl (2E,6E)-farnesoate + S-adenosyl-L-homocysteine
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
3.32 μM | juvenile hormone III acid | |||||
0.18 μM | farnesoate |
kcat is 7.7 min-1 for juvenile hormone III acid. kcat is 10.1 min-1 for farnesoate. kcat is 0.103 min-1 for laureate acid. kcat is 0.041 min-1 for palmitate acid.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Molecular Function | 3,4-dihydroxy-5-polyprenylbenzoic acid O-methyltransferase activity | |
Molecular Function | farnesoic acid O-methyltransferase activity | |
Molecular Function | juvenile hormone acid methyltransferase activity | |
Biological Process | juvenile hormone biosynthetic process | |
Biological Process | male courtship behavior | |
Biological Process | methylation | |
Biological Process | ubiquinone biosynthetic process |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameJuvenile hormone acid O-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ9VJK8
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000433619 | 1-297 | Juvenile hormone acid O-methyltransferase | |||
Sequence: MNQASLYQHANQVQRHDAKLILDEFASTMQWRSDGEDALLDVGSGSGNVLMDFVKPLLPIRGQLVGTDISSQMVHYASKHYQREERTRFQVLDIGCERLPEELSGRFDHVTSFYCLHWVQNLKGALGNIYNLLKPEGGDCLLAFLASNPVYEVYKILKTNDKWSTFMQDVENFISPLHYSLSPGEEFSQLLNDVGFVQHNVEIRNEVFVYEGVRTLKDNVKAICPFLERMPADLHEQFLDDFIDIVISMNLQQGENNEDQKFLSPYKLVVAYARKTPEFVNNVFLEPTHQNLVKGIN |
Proteomic databases
Expression
Tissue specificity
Predominantly expressed in corpora allata. Also expressed at low level in testis.
Developmental stage
Highly expressed in the first instar larvae and then gradually decreases during larval development. Expression increases again in the wandering third larval stage. The lowest amount of expression is observed in the early and mid-pupal stages. After this, expression increases in the late pupal stage in both male and female adults.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length297
- Mass (Da)34,065
- Last updated2002-10-01 v2
- Checksum9ACFBB7E0472E010
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 166 | in Ref. 1; BAC98836 and 4; AAL68063 | ||||
Sequence: F → Y | ||||||
Sequence conflict | 172 | in Ref. 4; AAL68063 | ||||
Sequence: N → S | ||||||
Sequence conflict | 182 | in Ref. 1; BAC98836 and 4; AAL68063 | ||||
Sequence: S → N |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB113579 EMBL· GenBank· DDBJ | BAC98836.1 EMBL· GenBank· DDBJ | mRNA | ||
AE014134 EMBL· GenBank· DDBJ | AAF53533.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014134 EMBL· GenBank· DDBJ | AHN54494.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY075194 EMBL· GenBank· DDBJ | AAL68063.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |