Q9VG96 · GSTD4_DROME
- ProteinGlutathione S-transferase D4
- GeneGstD4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids215 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028).
May be involved in detoxification (PubMed:22082028).
May be involved in detoxification (PubMed:22082028).
Catalytic activity
- glutathione + RX = a halide anion + an S-substituted glutathione + H+
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
2.47 mM | glutathione | |||||
2.04 mM | 1-chloro-2,4-dinitrobenzene |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
0.26 μmol/min/mg | with 1-chloro-2,4-dinitrobenzene as substrate | ||||
1.45 μmol/min/mg | with 4-hydroxy-2-nonenal as substrate | ||||
99.5 nmol/min/mg | with adrenochrome as substrate | ||||
0.044 μmol/min/mg | with phenethyl isothiocyanate as substrate | ||||
0.047 μmol/min/mg | with 2-hydroxyethyl disulfide as substrate |
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | glutathione transferase activity | |
Biological Process | glutathione metabolic process | |
Biological Process | response to toxic substance |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutathione S-transferase D4
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ9VG96
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000185956 | 1-215 | Glutathione S-transferase D4 | |||
Sequence: MDFYYSPRSSGSRTIIMVAKALGLELNKKQLRITEGEHLKPEFLKLNPQHTIPTLVDNGFAIWESRAIAVYLVEKYGKDDSLFPNDPQKRALINQRLYFDMGTLHDSFMKYYYPFIRTGQLGNAENYKKVEAAFEFLDIFLEGQDYVAGSQLTVADIAILSSVSTFEVVEFDISKYPNVARWYANAKKITPGWDENWKGLLQMKTMYEAQKASLK |
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
Homodimer.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9VG96 | GstD6 Q9VG94 | 3 | EBI-117276, EBI-152218 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-80 | GST N-terminal | ||||
Sequence: MDFYYSPRSSGSRTIIMVAKALGLELNKKQLRITEGEHLKPEFLKLNPQHTIPTLVDNGFAIWESRAIAVYLVEKYGKDD | ||||||
Domain | 86-207 | GST C-terminal | ||||
Sequence: DPQKRALINQRLYFDMGTLHDSFMKYYYPFIRTGQLGNAENYKKVEAAFEFLDIFLEGQDYVAGSQLTVADIAILSSVSTFEVVEFDISKYPNVARWYANAKKITPGWDENWKGLLQMKTMY |
Sequence similarities
Belongs to the GST superfamily. Delta family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length215
- Mass (Da)24,736
- Last updated2000-05-01 v1
- Checksum9556E0A1A54BBE1F
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 142 | in Ref. 1; M97702 | ||||
Sequence: E → V |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M97702 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AE014297 EMBL· GenBank· DDBJ | AAF54789.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY071648 EMBL· GenBank· DDBJ | AAL49270.1 EMBL· GenBank· DDBJ | mRNA |