Q9V292 · PRIS_PYRAB
- ProteinDNA primase small subunit PriS
- GenepriS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids345 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own, synthesizing DNA strands up to 3 kB. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability for RNA fragments up to 150 bases. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair. Displays gap-filling and strand-displacement activities.
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
60.5 mM | dNTPs | in the presence of 5 mM Mg2+ | ||||
5 mM | dNTPs | in the presence of 10 mM Mg2+ | ||||
198 mM | dNTPs | in the presence of 10 mM Mg2+ 5 mM Mn2+ | ||||
27.5 mM | NTPs | in the presence of 10 mM Mg2+ |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
1.12 pmol/min/mg | for dNTPs (in the presence of 5 mM Mg2+) | ||||
0.7 pmol/min/mg | for dNTPs (in the presence of 10 mM Mg2+) | ||||
5.9 pmol/min/mg | for dNTPs (in the presence of 10 mM Mg2+ and 5 mM Mn2+) | ||||
0.88 pmol/min/mg | for NTPs (in the presence of 5 mM Mg2+) |
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 95 | |||||
Sequence: D | ||||||
Active site | 97 | |||||
Sequence: D | ||||||
Binding site | 106 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 108 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 114 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 117 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Active site | 280 | |||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | DNA-directed RNA polymerase complex | |
Cellular Component | primosome complex | |
Molecular Function | DNA primase activity | |
Molecular Function | metal ion binding |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA primase small subunit PriS
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Thermococci > Thermococcales > Thermococcaceae > Pyrococcus
Accessions
- Primary accessionQ9V292
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000046748 | 1-345 | DNA primase small subunit PriS | |||
Sequence: MLLREVTKEERKEFYSNEWNAKQIPDFILQNLDKREFGFDHTGEGPSDRKNSYTDVRDLEDYIKATAPYAVYSSVAFYEKPQEMEGWLGAELVFDIDAKDLPLRRCNHEPGKVCPICLNDAKEIARDTLIVLKEELGFEDVHVVYSGRGYHIRVMDGWALSLDSKSRERILSFISASEIEDHSEFRKMLLERRGWFVLNHGYPRVFRLRFGYFILRVKVEHLINFGIRKNIAKRILDNKETIYEEFVRKGILAAFPDGVGIESLAKLFALSTRFSKAYFDGRVTVDLKRILRLPSTLHSKVGLIAKYIGNNERDVMRFNPFKHAVPKFRRKEVKEEYKRFLEENF |
Interaction
Subunit
Heterodimer of a small subunit (PriS) and a large subunit (PriL).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 106-117 | Zinc knuckle motif | ||||
Sequence: CNHEPGKVCPIC |
Sequence similarities
Belongs to the eukaryotic-type primase small subunit family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length345
- Mass (Da)40,508
- Last updated2000-05-01 v1
- Checksum782D8FBA1D71FEDF
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ248283 EMBL· GenBank· DDBJ | CAB49106.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
HE613800 EMBL· GenBank· DDBJ | CCE69558.1 EMBL· GenBank· DDBJ | Genomic DNA |