Q9V011 · SYM_PYRAB
- ProteinMethionine--tRNA ligase
- GenemetG
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids722 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.
Catalytic activity
- ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | methionine-tRNA ligase activity | |
Molecular Function | tRNA binding | |
Biological Process | methionyl-tRNA aminoacylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMethionine--tRNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Thermococci > Thermococcales > Thermococcaceae > Pyrococcus
Accessions
- Primary accessionQ9V011
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000139194 | 1-722 | Methionine--tRNA ligase | |||
Sequence: MVRYMVTSALPYANGPIHAGHLAGAYLPADIFVRYLRLKGEDVVFICGTDEHGTPISFRALKEGRSPREIVDEFHEQIKITFQRAKISFDFFGRTELPIHYKLSQEFFLKAYENGHLVKKVTKQAYCEHDKMFLPDRFVIGTCPYCGAEDQKGDQCEVCGRPLTPEILINPRCAICGRPISFRDSAHYYIKMQDFAERLKRWIEKQPWKPNVKNMVLSWIEEGLEERAITRDLNWGIPVPLDEEDMKGKVLYVWFEAPIGYISITIEHFKRIGKPNEWKKYWLNIDGQTRVIHFIGKDNIPFHAIFWPAFLMAYGKYKDEEVEAEWNLPYDIPANEYLTLEGKKFSTSRNWAIWVHEFLDVFPADYLRYYLTTIMPETRDSDFSFSDFKVRINEELVNNLGNFVHRALTFVNRYFDGVVPERGELDELDREALEEIEKAFKEVGELIMNYRFKDALKRVMSLASFGNRYFDHKQPWKTAKEDKVRTGTTVNISLQIVKALGILLEPFLPDASEKIWHLLNLDEVKRWEFRELPAGHKVRKPEILFKKVTDDQIIYFILNYMAKGNPEGARILLDKYYKREDVIRVAKEKFGDEAEVVLRRVYKDIKLKEKKEGKEMYVKFDDFAKLDLRVGKIIEVKDHPNADKLYVVKVDLGDEVRTLVAGLKKYYKPEELLNRYVVVVANLEPKKLRGIGSQGMLLAADDGERVALLMPDKEVKLGAKVR |
Interaction
Structure
Family & Domains
Features
Showing features for motif, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 11-21 | 'HIGH' region | ||||
Sequence: PYANGPIHAGH | ||||||
Motif | 344-348 | 'KMSKS' region | ||||
Sequence: KFSTS | ||||||
Domain | 622-722 | tRNA-binding | ||||
Sequence: DFAKLDLRVGKIIEVKDHPNADKLYVVKVDLGDEVRTLVAGLKKYYKPEELLNRYVVVVANLEPKKLRGIGSQGMLLAADDGERVALLMPDKEVKLGAKVR |
Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length722
- Mass (Da)84,295
- Last updated2000-05-01 v1
- ChecksumA69078A91D5BE028
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ248286 EMBL· GenBank· DDBJ | CAB49895.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
HE613800 EMBL· GenBank· DDBJ | CCE70393.1 EMBL· GenBank· DDBJ | Genomic DNA |