Q9UVF8 · THI4_UROFA

Function

function

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.

Catalytic activity

Cofactor

Fe cation (UniProtKB | Rhea| CHEBI:24875 )

Note: Binds 1 Fe cation per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site91substrate
Binding site112-113substrate
Binding site120substrate
Binding site185substrate
Binding site223substrate
Binding site238substrate
Binding site290substrate
Binding site300-302substrate

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentnucleus
Molecular Functioniron ion binding
Molecular Functionpentosyltransferase activity
Biological Processthiamine biosynthetic process
Biological Processthiazole biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Thiamine thiazole synthase
  • EC number
  • Alternative names
    • Planta-induced protein 4
    • Thiazole biosynthetic enzyme

Gene names

    • Name
      THI2
    • Synonyms
      PIG4

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Basidiomycota > Pucciniomycotina > Pucciniomycetes > Pucciniales > Pucciniaceae > Uromyces

Accessions

  • Primary accession
    Q9UVF8

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000340571-338Thiamine thiazole synthase
Modified residue2212,3-didehydroalanine (Cys)

Post-translational modification

During the catalytic reaction, a sulfide is transferred from Cys-221 to a reaction intermediate, generating a dehydroalanine residue.

Expression

Tissue specificity

Highly expressed in haustoria, and only in low amounts in intercellular hyphae. Found in the basal hyphae of the uredia, but not in the pedicels and only at very low levels in uredospores.

Interaction

Subunit

Homooctamer.

Structure

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias1-39Polar residues
Region1-43Disordered

Sequence similarities

Belongs to the THI4 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    338
  • Mass (Da)
    36,213
  • Last updated
    2000-05-01 v1
  • Checksum
    C3F9611094861230
MSPVATESMYKPTTINQTAHQQAMDPLKSKQQSNATVNKPAFKPEPAVNLTPIKFAPIKEHQVQRAMVRRYFQDMEERAISDVIIVGAGSAGLSCAYALGTARPDLKITILESNVAPGGGCWLGGQLMSAMVCRKPADEFLDQVGVPYEDEGNFVVVKHAALFTSTVLSKVLAMPNVKMFNATACEDLIIKPCPINPGVQRIAGCVTNWTLVSLNHDHQSCMDPSTITAPLVCSFAGHDGPFGAFCVKRVASAGLSEGLGDMRPLDMERAEDHIANKTREILPGLIVGGMELSEFDGSARMGPTFGAMLLSGKRAAEVALQSLDRVKIEEGEVVGLAK

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-39Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AJ250427
EMBL· GenBank· DDBJ
CAB59856.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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