Q9UT23 · MPH1_SCHPO
- ProteinATP-dependent DNA helicase fml1
- Genefml1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids834 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
ATP-dependent DNA helicase involved in DNA damage repair by homologous recombination and in genome maintenance. Capable of unwinding D-loops (PubMed:18851838, PubMed:22723423, PubMed:22844101).
Plays a role in limiting crossover recombination during mitotic DNA double-strand break (DSB) repair (PubMed:18851838).
Component of a FANCM-MHF complex which promotes gene conversion at blocked replication forks, probably by reversal of the stalled fork (PubMed:18851838, PubMed:20347428).
FANCM-MHF also promotes non-crossover recombination in meiotic cells (PubMed:22723423).
Plays a role in limiting crossover recombination during mitotic DNA double-strand break (DSB) repair (PubMed:18851838).
Component of a FANCM-MHF complex which promotes gene conversion at blocked replication forks, probably by reversal of the stalled fork (PubMed:18851838, PubMed:20347428).
FANCM-MHF also promotes non-crossover recombination in meiotic cells (PubMed:22723423).
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | FANCM-MHF complex | |
Cellular Component | nucleolus | |
Cellular Component | nucleus | |
Cellular Component | site of double-strand break | |
Molecular Function | 3'-5' DNA helicase activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | four-way junction DNA binding | |
Molecular Function | four-way junction helicase activity | |
Biological Process | DNA strand displacement | |
Biological Process | double-strand break repair via synthesis-dependent strand annealing | |
Biological Process | interstrand cross-link repair | |
Biological Process | meiotic strand displacement involved in double-strand break repair via SDSA | |
Biological Process | Okazaki fragment processing involved in mitotic DNA replication | |
Biological Process | reciprocal meiotic recombination | |
Biological Process | recombinational interstrand cross-link repair | |
Biological Process | replication fork processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent DNA helicase fml1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Taphrinomycotina > Schizosaccharomycetes > Schizosaccharomycetales > Schizosaccharomycetaceae > Schizosaccharomyces
Accessions
- Primary accessionQ9UT23
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Hypersensitive to the alkylating agent methyl methanesulfonate (MMS) and the cross-linking agent cisplatin.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 99 | Retains DNA-binding activity but is unable to unwind D loops or dissociate synthetic HJs. | ||||
Sequence: K → R | ||||||
Mutagenesis | 196 | Retains DNA-binding activity but is unable to unwind D loops or dissociate synthetic HJs. | ||||
Sequence: D → N |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 20 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000310751 | 1-834 | ATP-dependent DNA helicase fml1 | |||
Sequence: MSDDSFSSDEDWDELDTQVVDKIENEYHNNTIGLNGYSVDEYFDANDSNRYRLQHELDESAAQQWVYPINVSFRDYQFNIVQKALFENVLVALPTGLGKTFIAAVVMMNYLRWFPKSYIVFMAPTKPLVTQQMEACYKITGIPKSQTAELSGHVPVTTRNQYYQSRNVFFVTPQTILNDIKHGICDRTRISCLVIDEAHRSTGNYAYVEVVHLLSLSNKNFRILALSATPGNKLEAIQNVIDSLHISRIEIRTENSIDISQYVQKKEVDFFPVDLSAEITDIRDRFSSILEPMLQKLNKGNYYRIQNAKDITSFTVVQAKQAFLAMSGQNFPANQKWDILNTFDALATFAYPLNLLLNHGIRPFYQKLREVEEECFVGRSGYKKRIINHENYRPLMDDIEILLRDQSFVGHPKLEHLERIVTEYFEKEQTKDTRIMIFVEIRSSAEEILRFLGKFYPNVRPAIFIGQSAVRKAAGMSQKLQNETVKQFQKGEVNTLIATSIGEEGLDIGEVDMIICYDASASPIRMLQRMGRTGRKRKGYIYMLLTRGKEEAKWERAKDAYRTLQDNIVSGRGLSLSEKSYRILPEKFRPVCDKRVIEIPKENEEVVVAPKKVQLRTKIKKKFFMPENALNGFITASALGKPKRALAKSEESPFEICPVTYSIEQEKKLEKYKRVCLRGLDIHRNRRLSQLSVTGRIPHSLATKSIHSFLKHLNTIDSQKAQEWRREINNQFQVSNINSTDRDTKQPKMHDFRQPLHPNPMTTLKRKGQHNSFSYDKSTLFYDNNNNLEEDLPDVNISISSRNEEASKTKPFDDRQQRLQQLVEKRKRMKGMLI |
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for domain, motif, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 80-248 | Helicase ATP-binding | ||||
Sequence: IVQKALFENVLVALPTGLGKTFIAAVVMMNYLRWFPKSYIVFMAPTKPLVTQQMEACYKITGIPKSQTAELSGHVPVTTRNQYYQSRNVFFVTPQTILNDIKHGICDRTRISCLVIDEAHRSTGNYAYVEVVHLLSLSNKNFRILALSATPGNKLEAIQNVIDSLHISR | ||||||
Motif | 196-199 | DEAH box | ||||
Sequence: DEAH | ||||||
Domain | 416-582 | Helicase C-terminal | ||||
Sequence: HLERIVTEYFEKEQTKDTRIMIFVEIRSSAEEILRFLGKFYPNVRPAIFIGQSAVRKAAGMSQKLQNETVKQFQKGEVNTLIATSIGEEGLDIGEVDMIICYDASASPIRMLQRMGRTGRKRKGYIYMLLTRGKEEAKWERAKDAYRTLQDNIVSGRGLSLSEKSYR | ||||||
Region | 650-690 | Interaction with MHF complex | ||||
Sequence: EESPFEICPVTYSIEQEKKLEKYKRVCLRGLDIHRNRRLSQ | ||||||
Region | 738-769 | Disordered | ||||
Sequence: NSTDRDTKQPKMHDFRQPLHPNPMTTLKRKGQ |
Sequence similarities
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length834
- Mass (Da)96,555
- Last updated2006-02-21 v2
- Checksum987AD715109C8215
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CU329670 EMBL· GenBank· DDBJ | CAB57423.2 EMBL· GenBank· DDBJ | Genomic DNA |