Q9USU3 · FBH1_SCHPO

Function

function

Involved in ATP-dependent DNA-unwinding in a 3' to 5' direction, and ATP-ase activities stimulated by the single-stranded DNA-binding protein ssb1. Essential for viability and normal growth of stationary phase cells and in the absence of either srs2 or rqh1 DNA helicase (PubMed:16135799, PubMed:9228070).
Involved in DNA recombination repair of strand breaks and stalled or collapsed replication forks, on the rhp51-dependent pathway: promotes rhp51 filament dissolution from stalled forks, thereby inhibiting homologous recombination and preventing excessive recombination (PubMed:16135800, PubMed:19546232).
Ubiquitination and DNA helicase activities are essential for controlling rhp51-dependent recombination in the absence of rad22. Plays a role in the processing of toxic recombination intermediates. Promotes proper chromosome segregation (PubMed:16135799, PubMed:16135800, PubMed:19546232, PubMed:9228070).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Pathway

Protein modification; protein ubiquitination.

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentnuclear SCF ubiquitin ligase complex
Cellular Componentnucleus
Cellular Componentsingle-stranded DNA-dependent ATP-dependent DNA helicase complex
Molecular Function3'-5' DNA helicase activity
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functionisomerase activity
Molecular Functionsingle-stranded 3'-5' DNA helicase activity
Molecular Functionsingle-stranded DNA binding
Molecular Functionubiquitin-like ligase-substrate adaptor activity
Biological ProcessDNA recombination
Biological Processdouble-strand break repair via homologous recombination
Biological Processmitotic recombination-dependent replication fork processing
Biological Processprotein ubiquitination
Biological Processregulation of DNA recombination
Biological Processreplication fork processing
Biological Processresolution of meiotic recombination intermediates
Biological ProcessSCF-dependent proteasomal ubiquitin-dependent protein catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    F-box DNA helicase protein 1
  • EC number
  • Alternative names
    • DNA 3'-5' helicase 1

Gene names

    • Name
      fbh1
    • Synonyms
      fdh
      , fdh1
    • ORF names
      SPBC336.01

Organism names

Accessions

  • Primary accession
    Q9USU3

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis14Modest reduction in DNA helicase activity; when in combination with A-15.
Mutagenesis15Modest reduction in DNA helicase activity; when in combination with A-14.
Mutagenesis485Prevents DNA helicase activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002714301-878F-box DNA helicase protein 1

Proteomic databases

Interaction

Subunit

Part of the E3 ubiquitin ligase Skp1-Cullin-1-F-box (SCF) complex. Interacts with skp1 and ssb1.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain8-56F-box

Sequence similarities

Belongs to the helicase family. UvrD subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    878
  • Mass (Da)
    99,705
  • Last updated
    2001-10-01 v2
  • Checksum
    E051D904F8293C15
MSAQHLHSCKFYRLPLEIIPLICRFLSVQDIQSFIRVFPSFQTILDSSNDLFWKKKNYELRIRRNRLLRGSYAAGVSSSAMNGFVNGTQISSTPAEREYYSKSDEIKNICGLPPGPMKVEQIGHAIDHLVSETHTVDHLGSSIKASFFHIDDVPLEWISSICMQVQSFFSPAAREAISSLKSRTTTSNLLLSLFVGILFEDDLWYFFNTLYMLSSTSAIEFAYFLDSIFTVVRTDYEHYRDPLSQTLITSCTRIHNIVAVIESPYDEPNTKGLTSEQKMIVECQLNPGEVLKVKAFAGTGKTKALLEFAKSRPKDKILYVAFNKAAKEDAELRFPFNVKCSTMHGLAYGAILAQADLPQAKLERQLSNSTIASLLSLQVAFPKANRKNNPGTPSASLVASHIMFTLNRFMHSTDWQLGFRHISKRSLEVTKLSKEKLLAYTKKLWSLIVNFEYTHAPLIPDAYMKLLHLYEFPNIFSKYDYILFDEAQDFTPCMVDLIYRQKHARIVIVGDAHQCIYGFRGANACAFNENLYPSTKQLCLTKSFRFGNSVAKYANFLLSLKGENVKLKGVQNDHAYWSSASNPNNVSGAFRFFPHTIIFRTNKELILQSIRLSVSLPKEIPIAILGSMRKKAFQLLRSGSELAHGQRPSHPKLKDFSSWGEFEVHVKNSAEEDAELALVYDMADELFSESFLSRLDNCEKRLMDSKDDGDNGIILATAHQSKGLEWDNVQLGNDFRPKFDSVSFSRIGSSRYLQEEINILYVALTRAKKRLILNDTITKLYALECGLVRFAGGILTEDQLQPGKVALFVDWQIDKFSFFYETPAEGYNLLVEANEKSVWDIFFGVLSGAWQNYIANTSERLKRSMLFIENQLFAVHDQ

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF380117
EMBL· GenBank· DDBJ
AAK58077.1
EMBL· GenBank· DDBJ
Genomic DNA
CU329671
EMBL· GenBank· DDBJ
CAC44128.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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