Q9USU3 · FBH1_SCHPO
- ProteinF-box DNA helicase protein 1
- Genefbh1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids878 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in ATP-dependent DNA-unwinding in a 3' to 5' direction, and ATP-ase activities stimulated by the single-stranded DNA-binding protein ssb1. Essential for viability and normal growth of stationary phase cells and in the absence of either srs2 or rqh1 DNA helicase (PubMed:16135799, PubMed:9228070).
Involved in DNA recombination repair of strand breaks and stalled or collapsed replication forks, on the rhp51-dependent pathway: promotes rhp51 filament dissolution from stalled forks, thereby inhibiting homologous recombination and preventing excessive recombination (PubMed:16135800, PubMed:19546232).
Ubiquitination and DNA helicase activities are essential for controlling rhp51-dependent recombination in the absence of rad22. Plays a role in the processing of toxic recombination intermediates. Promotes proper chromosome segregation (PubMed:16135799, PubMed:16135800, PubMed:19546232, PubMed:9228070).
Involved in DNA recombination repair of strand breaks and stalled or collapsed replication forks, on the rhp51-dependent pathway: promotes rhp51 filament dissolution from stalled forks, thereby inhibiting homologous recombination and preventing excessive recombination (PubMed:16135800, PubMed:19546232).
Ubiquitination and DNA helicase activities are essential for controlling rhp51-dependent recombination in the absence of rad22. Plays a role in the processing of toxic recombination intermediates. Promotes proper chromosome segregation (PubMed:16135799, PubMed:16135800, PubMed:19546232, PubMed:9228070).
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Pathway
Protein modification; protein ubiquitination.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | nuclear SCF ubiquitin ligase complex | |
Cellular Component | nucleus | |
Cellular Component | single-stranded DNA-dependent ATP-dependent DNA helicase complex | |
Molecular Function | 3'-5' DNA helicase activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | isomerase activity | |
Molecular Function | single-stranded 3'-5' DNA helicase activity | |
Molecular Function | single-stranded DNA binding | |
Molecular Function | ubiquitin-like ligase-substrate adaptor activity | |
Biological Process | DNA recombination | |
Biological Process | double-strand break repair via homologous recombination | |
Biological Process | mitotic recombination-dependent replication fork processing | |
Biological Process | protein ubiquitination | |
Biological Process | regulation of DNA recombination | |
Biological Process | replication fork processing | |
Biological Process | resolution of meiotic recombination intermediates | |
Biological Process | SCF-dependent proteasomal ubiquitin-dependent protein catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameF-box DNA helicase protein 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Taphrinomycotina > Schizosaccharomycetes > Schizosaccharomycetales > Schizosaccharomycetaceae > Schizosaccharomyces
Accessions
- Primary accessionQ9USU3
Proteomes
Organism-specific databases
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 14 | Modest reduction in DNA helicase activity; when in combination with A-15. | |||
Mutagenesis | 15 | Modest reduction in DNA helicase activity; when in combination with A-14. | |||
Mutagenesis | 485 | Prevents DNA helicase activity. | |||
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000271430 | 1-878 | F-box DNA helicase protein 1 | ||
Proteomic databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 8-56 | F-box | |||
Sequence similarities
Belongs to the helicase family. UvrD subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length878
- Mass (Da)99,705
- Last updated2001-10-01 v2
- ChecksumE051D904F8293C15
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF380117 EMBL· GenBank· DDBJ | AAK58077.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CU329671 EMBL· GenBank· DDBJ | CAC44128.1 EMBL· GenBank· DDBJ | Genomic DNA |