Q9UMR5 · PPT2_HUMAN
- ProteinLysosomal thioesterase PPT2
- GenePPT2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids302 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Removes thioester-linked fatty acyl groups from various substrates including S-palmitoyl-CoA. Has the highest S-thioesterase activity for the acyl groups palmitic and myristic acid followed by other short- and long-chain acyl substrates. However, because of structural constraints, is unable to remove palmitate from peptides or proteins.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
67 μM | S-palmitoyl-CoA | |||||
37 μM | S-palmitoyl-N-acetylcysteamine | |||||
117 μM | 4-methylumbelliferyl-6-S-palmitoyl-beta-D-glucopyranoside |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
1.7 μmol/min/mg | toward S-palmitoyl-CoA | ||||
3.3 μmol/min/mg | toward S-palmitoyl-N-acetylcysteamine | ||||
0.43 μmol/min/mg | toward 4-methylumbelliferyl-6-S-palmitoyl-beta-D-glucopyranoside |
pH Dependence
Optimum pH is 7.0.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 111 | Nucleophile | ||||
Sequence: S | ||||||
Active site | 228 | |||||
Sequence: D | ||||||
Active site | 283 | |||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular exosome | |
Cellular Component | lysosomal lumen | |
Cellular Component | lysosome | |
Molecular Function | palmitoyl hydrolase activity | |
Molecular Function | thiolester hydrolase activity | |
Biological Process | fatty-acyl-CoA biosynthetic process |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameLysosomal thioesterase PPT2
- EC number
- Short namesPPT-2
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ9UMR5
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Disease & Variants
Features
Showing features for natural variant, mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_027107 | 5 | in dbSNP:rs3134604 | |||
Sequence: C → W | ||||||
Natural variant | VAR_027108 | 34 | in dbSNP:rs3096696 | |||
Sequence: A → E | ||||||
Mutagenesis | 111 | Abolishes enzymatic activity. | ||||
Sequence: S → A | ||||||
Mutagenesis | 228 | Abolishes enzymatic activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 283 | Abolishes enzymatic activity. | ||||
Sequence: H → A | ||||||
Mutagenesis | 287 | No effect on enzymatic activity. | ||||
Sequence: H → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 267 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for signal, chain, glycosylation, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-27 | |||||
Sequence: MLGLCGQRLPAAWVLLLLPFLPLLLLA | ||||||
Chain | PRO_0000025554 | 28-302 | Lysosomal thioesterase PPT2 | |||
Sequence: APAPHRASYKPVIVVHGLFDSSYSFRHLLEYINETHPGTVVTVLDLFDGRESLRPLWEQVQGFREAVVPIMAKAPQGVHLICYSQGGLVCRALLSVMDDHNVDSFISLSSPQMGQYGDTDYLKWLFPTSMRSNLYRICYSPWGQEFSICNYWHDPHHDDLYLNASSFLALINGERDHPNATVWRKNFLRVGHLVLIGGPDDGVITPWQSSFFGFYDANETVLEMEEQLVYLRDSFGLKTLLARGAIVRCPMAGISHTAWHSNRTLYETCIEPWLS | ||||||
Glycosylation | 60 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 109↔117 | |||||
Sequence: CYSQGGLVC | ||||||
Disulfide bond | 165↔176 | |||||
Sequence: CYSPWGQEFSIC | ||||||
Glycosylation | 190 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 206 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 245 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 276↔296 | |||||
Sequence: CPMAGISHTAWHSNRTLYETC | ||||||
Glycosylation | 289 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Broadly expressed, with highest levels in skeletal muscle.
Gene expression databases
Organism-specific databases
Structure
Sequence & Isoforms
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 3 isoforms produced by Alternative splicing.
Q9UMR5-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length302
- Mass (Da)34,225
- Last updated2010-05-18 v4
- ChecksumE242D677970B4BEA
Q9UMR5-2
- Name2
- SynonymsI
- NoteCatalytically inactive due to lack of His-283. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
- Differences from canonical
- 256-300: VYLRDSFGLKTLLARGAIVRCPMAGISHTAWHSNRTLYETCIEPW → PARPTHQSELLLLRLVCLKPPRRKKPACRVQRQSESWGPGLSCA
Q9UMR5-3
- Name3
- Differences from canonical
- 1-1: M → MKSCGSM
Computationally mapped potential isoform sequences
There are 23 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A2ABN6 | A2ABN6_HUMAN | PPT2 | 97 | ||
A2ABN7 | A2ABN7_HUMAN | PPT2 | 158 | ||
G8JLL2 | G8JLL2_HUMAN | PPT2 | 180 | ||
H3BNV4 | H3BNV4_HUMAN | PPT2-EGFL8 | 129 | ||
A0A140T9Y1 | A0A140T9Y1_HUMAN | PPT2 | 242 | ||
A0A140T8X7 | A0A140T8X7_HUMAN | PPT2 | 308 | ||
A0A024RCX6 | A0A024RCX6_HUMAN | PPT2 | 302 | ||
A0A140TA16 | A0A140TA16_HUMAN | PPT2 | 54 | ||
A0A0G2JK02 | A0A0G2JK02_HUMAN | PPT2 | 180 | ||
A0A0G2JK07 | A0A0G2JK07_HUMAN | PPT2 | 158 | ||
A0A0G2JLK3 | A0A0G2JLK3_HUMAN | PPT2 | 39 | ||
A0A0G2JLK6 | A0A0G2JLK6_HUMAN | PPT2 | 132 | ||
A0A0G2JLK8 | A0A0G2JLK8_HUMAN | PPT2 | 97 | ||
A0A0G2JLK9 | A0A0G2JLK9_HUMAN | PPT2 | 127 | ||
A0A0G2JLL3 | A0A0G2JLL3_HUMAN | 301 | |||
A0A0G2JKR3 | A0A0G2JKR3_HUMAN | PPT2 | 236 | ||
A0A0G2JLA3 | A0A0G2JLA3_HUMAN | 301 | |||
A0A0G2JID8 | A0A0G2JID8_HUMAN | PPT2 | 158 | ||
A0A0G2JI52 | A0A0G2JI52_HUMAN | PPT2 | 93 | ||
A0A0G2JIU6 | A0A0G2JIU6_HUMAN | PPT2 | 180 | ||
A0A0G2JHI0 | A0A0G2JHI0_HUMAN | PPT2 | 93 | ||
A0A0G2JHB6 | A0A0G2JHB6_HUMAN | PPT2 | 200 | ||
A0A0G2JH76 | A0A0G2JH76_HUMAN | PPT2 | 302 |
Sequence caution
Features
Showing features for alternative sequence, sequence conflict.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF020543 EMBL· GenBank· DDBJ | AAB80730.1 EMBL· GenBank· DDBJ | mRNA | ||
AF020544 EMBL· GenBank· DDBJ | AAB80731.1 EMBL· GenBank· DDBJ | mRNA | ||
Y17958 EMBL· GenBank· DDBJ | CAB46981.1 EMBL· GenBank· DDBJ | mRNA | ||
AL110128 EMBL· GenBank· DDBJ | CAB53659.1 EMBL· GenBank· DDBJ | mRNA | ||
CR533546 EMBL· GenBank· DDBJ | CAG38577.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AK292729 EMBL· GenBank· DDBJ | BAF85418.1 EMBL· GenBank· DDBJ | mRNA | ||
U89336 EMBL· GenBank· DDBJ | AAB47495.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL662828 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL662884 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL845464 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BX284686 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BX927239 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CR753803 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CR812478 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CR933878 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH471081 EMBL· GenBank· DDBJ | EAX03597.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH471081 EMBL· GenBank· DDBJ | EAX03591.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC001355 EMBL· GenBank· DDBJ | AAH01355.1 EMBL· GenBank· DDBJ | mRNA |