Q9UJA5 · TRM6_HUMAN
- ProteintRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
- GeneTRMT6
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids497 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Substrate-binding subunit of tRNA (adenine-N1-)-methyltransferase, which catalyzes the formation of N1-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA (PubMed:16043508).
Together with the TRMT61A catalytic subunit, part of a mRNA N1-methyltransferase complex that mediates methylation of adenosine residues at the N1 position of a small subset of mRNAs: N1 methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries (PubMed:29072297, PubMed:29107537).
Together with the TRMT61A catalytic subunit, part of a mRNA N1-methyltransferase complex that mediates methylation of adenosine residues at the N1 position of a small subset of mRNAs: N1 methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries (PubMed:29072297, PubMed:29107537).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 94-104 | substrate | ||||
Sequence: NRNIVDDGKSQ | ||||||
Binding site | 145-154 | substrate | ||||
Sequence: KYIKKKKKKY | ||||||
Binding site | 175-182 | substrate | ||||
Sequence: REPGKINH | ||||||
Binding site | 349 | substrate | ||||
Sequence: R | ||||||
Binding site | 377 | substrate | ||||
Sequence: R | ||||||
Binding site | 415-423 | substrate | ||||
Sequence: RERGGVINL | ||||||
Binding site | 434-441 | substrate | ||||
Sequence: QVLPDRSH |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Cellular Component | tRNA (m1A) methyltransferase complex | |
Molecular Function | RNA binding | |
Biological Process | mRNA processing | |
Biological Process | tRNA methylation |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ9UJA5
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_053789 | 293 | in dbSNP:rs6139876 | |||
Sequence: E → K | ||||||
Natural variant | VAR_053790 | 299 | in dbSNP:rs451571 | |||
Sequence: E → G | ||||||
Natural variant | VAR_053791 | 333 | in dbSNP:rs35203742 | |||
Sequence: P → L |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 513 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000233098 | 1-497 | UniProt | tRNA (adenine58-N1)-methyltransferase non-catalytic subunit TRM6 | |||
Sequence: MEGSGEQPGPQPQHPGDHRIRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTSGGSLQPKKKREEPTAETKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEEIVQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHMRYDTLAQMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGPVRAATACFGFPKSFLSGLYEFPLNKVDSLLHGTFSAKMLSSEPKDSALVEESNGTLEEKQASEQENEDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQRKRHLEAAALLSERNADGLIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVINLRLSETWLRNYQVLPDRSHPKLLMSGGGGYLLSGFTVAMDNLKADTSLKSNASTLESHETEEPAAKKRKCPESDS | |||||||
Modified residue | 107 | UniProt | Phosphothreonine | ||||
Sequence: T | |||||||
Modified residue (large scale data) | 288 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 291 | PRIDE | Phosphothreonine | ||||
Sequence: T | |||||||
Modified residue | 298 | UniProt | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue | 305 | UniProt | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 475 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 476 | PRIDE | Phosphothreonine | ||||
Sequence: T |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Heterotetramer; composed of two copies of TRMT6 and two copies of TRMT61A.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9UJA5 | KAT5 Q92993 | 3 | EBI-934061, EBI-399080 | |
BINARY | Q9UJA5 | LMO3 Q8TAP4-4 | 3 | EBI-934061, EBI-11742507 | |
BINARY | Q9UJA5 | PPIA P62937-2 | 3 | EBI-934061, EBI-25884072 | |
BINARY | Q9UJA5 | SETDB1 Q15047-2 | 3 | EBI-934061, EBI-9090795 | |
BINARY | Q9UJA5 | TRMT61A Q96FX7 | 4 | EBI-934061, EBI-934042 | |
BINARY | Q9UJA5 | YWHAG P61981 | 3 | EBI-934061, EBI-359832 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-20 | Disordered | ||||
Sequence: MEGSGEQPGPQPQHPGDHRI | ||||||
Region | 69-100 | Disordered | ||||
Sequence: TSGGSLQPKKKREEPTAETKEAGTDNRNIVDD | ||||||
Compositional bias | 74-100 | Basic and acidic residues | ||||
Sequence: LQPKKKREEPTAETKEAGTDNRNIVDD | ||||||
Region | 276-354 | Disordered | ||||
Sequence: SSEPKDSALVEESNGTLEEKQASEQENEDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQR | ||||||
Compositional bias | 292-354 | Basic and acidic residues | ||||
Sequence: LEEKQASEQENEDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQR | ||||||
Region | 472-497 | Disordered | ||||
Sequence: SNASTLESHETEEPAAKKRKCPESDS | ||||||
Compositional bias | 477-497 | Basic and acidic residues | ||||
Sequence: LESHETEEPAAKKRKCPESDS |
Sequence similarities
Belongs to the TRM6/GCD10 family.
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 4 isoforms produced by Alternative splicing.
Q9UJA5-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length497
- Mass (Da)55,799
- Last updated2000-05-01 v1
- Checksum920800D0722A6CBB
Q9UJA5-2
- Name2
- Differences from canonical
- 372-497: LIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVINLRLSETWLRNYQVLPDRSHPKLLMSGGGGYLLSGFTVAMDNLKADTSLKSNASTLESHETEEPAAKKRKCPESDS → CVDGRRQETPQCMWALLQTDWHSLSPCL
Q9UJA5-3
- Name3
Q9UJA5-4
- Name4
- Differences from canonical
- 1-170: Missing
Sequence caution
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_054740 | 1-170 | in isoform 4 | |||
Sequence: Missing | ||||||
Compositional bias | 74-100 | Basic and acidic residues | ||||
Sequence: LQPKKKREEPTAETKEAGTDNRNIVDD | ||||||
Compositional bias | 292-354 | Basic and acidic residues | ||||
Sequence: LEEKQASEQENEDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQR | ||||||
Alternative sequence | VSP_031100 | 344-365 | in isoform 3 | |||
Sequence: QEKQRRQEEQRKRHLEAAALLS → SGKNRGRQGRSSGKDFWGCRFA | ||||||
Alternative sequence | VSP_031101 | 366-497 | in isoform 3 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_018025 | 372-497 | in isoform 2 | |||
Sequence: LIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVINLRLSETWLRNYQVLPDRSHPKLLMSGGGGYLLSGFTVAMDNLKADTSLKSNASTLESHETEEPAAKKRKCPESDS → CVDGRRQETPQCMWALLQTDWHSLSPCL | ||||||
Compositional bias | 477-497 | Basic and acidic residues | ||||
Sequence: LESHETEEPAAKKRKCPESDS |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB032979 EMBL· GenBank· DDBJ | BAA86467.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AF132943 EMBL· GenBank· DDBJ | AAD27718.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AK000613 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
AK300812 EMBL· GenBank· DDBJ | BAG62468.1 EMBL· GenBank· DDBJ | mRNA | ||
AL035461 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC001262 EMBL· GenBank· DDBJ | AAH01262.1 EMBL· GenBank· DDBJ | mRNA |