Q9UIJ7 · KAD3_HUMAN

  • Protein
    GTP:AMP phosphotransferase AK3, mitochondrial
  • Gene
    AK3
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Mitochondrial adenylate kinase with a specific GTP:AMP phosphotransferase activity (PubMed:11485571, PubMed:32822537).
Could also use ITP as phosphate donor (PubMed:11485571).
Its physiological function is to recycle GTP into GDP which is necessary for the TCA cycle in the mitochondrial matrix (Probable)

Catalytic activity

Activity regulation

Inhibited by ATP.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
10 μMGTP
kcat is 57 sec-1 with GTP as phosphate donor.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site17GTP (UniProtKB | ChEBI)
Binding site19GTP (UniProtKB | ChEBI)
Binding site20GTP (UniProtKB | ChEBI)
Binding site21GTP (UniProtKB | ChEBI)
Binding site22GTP (UniProtKB | ChEBI)
Binding site38AMP (UniProtKB | ChEBI)
Binding site43AMP (UniProtKB | ChEBI)
Binding site64AMP (UniProtKB | ChEBI)
Binding site91AMP (UniProtKB | ChEBI)
Binding site94AMP (UniProtKB | ChEBI)
Binding site98AMP (UniProtKB | ChEBI)
Binding site128GTP (UniProtKB | ChEBI)
Binding site138GTP (UniProtKB | ChEBI)
Binding site139GTP (UniProtKB | ChEBI)
Binding site161GTP (UniProtKB | ChEBI)
Binding site172GTP (UniProtKB | ChEBI)
Binding site201GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentmitochondrial matrix
Cellular Componentmitochondrion
Molecular FunctionATP binding
Molecular FunctionGTP binding
Molecular Functionnucleoside triphosphate adenylate kinase activity
Biological ProcessADP biosynthetic process
Biological ProcessAMP metabolic process
Biological Processblood coagulation
Biological ProcessGTP metabolic process
Biological ProcessITP metabolic process
Biological Processnucleoside triphosphate biosynthetic process
Biological ProcessUTP metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    GTP:AMP phosphotransferase AK3, mitochondrial
  • EC number
  • Alternative names
    • Adenylate kinase 3
    • Adenylate kinase 3 alpha-like 1
    • Adenylate kinase isozyme 3

Gene names

    • Name
      AK3
    • Synonyms
      AK3L1
      , AK6
      , AKL3L

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo

Accessions

  • Primary accession
    Q9UIJ7
  • Secondary accessions
    • B4DP58
    • D3DRI1
    • E7ET30
    • Q5VYW6
    • Q9H576

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Disease & Variants

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 372 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Organism-specific databases

Miscellaneous

Genetic variation databases

PTM/Processing

Features

Showing features for chain, modified residue, modified residue (large scale data).

TypeIDPosition(s)SourceDescription
ChainPRO_00001589221-227UniProtGTP:AMP phosphotransferase AK3, mitochondrial
Modified residue20UniProtN6-succinyllysine
Modified residue (large scale data)22PRIDEPhosphothreonine
Modified residue34UniProtN6-acetyllysine
Modified residue37UniProtPhosphoserine
Modified residue (large scale data)37PRIDEPhosphoserine
Modified residue (large scale data)38PRIDEPhosphoserine
Modified residue57UniProtN6-succinyllysine
Modified residue64UniProtN6-acetyllysine; alternate
Modified residue64UniProtN6-succinyllysine; alternate
Modified residue80UniProtN6-acetyllysine; alternate
Modified residue80UniProtN6-succinyllysine; alternate
Modified residue174UniProtN6-acetyllysine; alternate
Modified residue174UniProtN6-succinyllysine; alternate
Modified residue189UniProtN6-acetyllysine; alternate
Modified residue189UniProtN6-succinyllysine; alternate
Modified residue203UniProtN6-acetyllysine

Keywords

Proteomic databases

2D gel databases

PTM databases

Expression

Tissue specificity

Highly expressed in heart, skeletal muscle and liver, moderately expressed in pancreas and kidney, and weakly expressed in placenta, brain and lung.

Gene expression databases

    • ENSG00000147853Expressed in cardiac muscle of right atrium and 194 other cell types or tissues

Organism-specific databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region37-66NMP
Region127-164LID

Domain

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP hydrolysis.

Sequence similarities

Belongs to the adenylate kinase family. AK3 subfamily.

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete

This entry describes 3 isoforms produced by Alternative splicing.

Q9UIJ7-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    227
  • Mass (Da)
    25,565
  • Last updated
    2007-01-23 v4
  • Checksum
    98A0EDF4FD9C9CEF
MGASARLLRAVIMGAPGSGKGTVSSRITTHFELKHLSSGDLLRDNMLRGTEIGVLAKAFIDQGKLIPDDVMTRLALHELKNLTQYSWLLDGFPRTLPQAEALDRAYQIDTVINLNVPFEVIKQRLTARWIHPASGRVYNIEFNPPKTVGIDDLTGEPLIQREDDKPETVIKRLKAYEDQTKPVLEYYQKKGVLETFSGTETNKIWPYVYAFLQTKVPQRSQKASVTP

Q9UIJ7-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Q9UIJ7-3

  • Name
    3
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Features

Showing features for alternative sequence, sequence conflict.

TypeIDPosition(s)Description
Alternative sequenceVSP_0430901-70in isoform 2
Sequence conflict5in Ref. 1; BAA87913
Sequence conflict38in Ref. 1; BAA87913
Alternative sequenceVSP_04487651-90in isoform 3
Sequence conflict57in Ref. 1; BAA87913
Sequence conflict69-71in Ref. 1; BAA87913
Sequence conflict181in Ref. 3; BAG60470

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB021870
EMBL· GenBank· DDBJ
BAA87913.1
EMBL· GenBank· DDBJ
mRNA
AF183419
EMBL· GenBank· DDBJ
AAG09688.1
EMBL· GenBank· DDBJ
mRNA
AK001553
EMBL· GenBank· DDBJ
BAA91753.1
EMBL· GenBank· DDBJ
mRNA
AK001951
EMBL· GenBank· DDBJ
BAA91996.1
EMBL· GenBank· DDBJ
mRNA
AK027534
EMBL· GenBank· DDBJ
BAB55183.1
EMBL· GenBank· DDBJ
mRNA
AK098205
EMBL· GenBank· DDBJ
BAG53592.1
EMBL· GenBank· DDBJ
mRNA
AK298200
EMBL· GenBank· DDBJ
BAG60470.1
EMBL· GenBank· DDBJ
mRNA
AL136231
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AL353151
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CH471071
EMBL· GenBank· DDBJ
EAW58779.1
EMBL· GenBank· DDBJ
Genomic DNA
CH471071
EMBL· GenBank· DDBJ
EAW58780.1
EMBL· GenBank· DDBJ
Genomic DNA
CH471071
EMBL· GenBank· DDBJ
EAW58781.1
EMBL· GenBank· DDBJ
Genomic DNA
BC013771
EMBL· GenBank· DDBJ
AAH13771.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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