Q9TYV5 · NSUN1_CAEEL
- Protein26S rRNA (cytosine-C(5))-methyltransferase nsun-1
- Genensun-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids664 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Methyltransferase which methylates the carbon-5 position of cytosine 2982 to 5-methylcytosine (m5C2982) in 26S rRNA (PubMed:33283887, PubMed:33289480).
May play a role in the translation of leucine and proline codons (Probable). May be required for the translation of specific mRNAs such as mRNAs involved in gonad development, collagen production and cuticle integrity (PubMed:33289480).
Plays a role in ensuring the correct localization of the germline-specific protein gld-1 during development (PubMed:33289480).
Not required for pre-rRNA processing, the production of mature 5S, 5.8S, 18S or 26S rRNAs or global translation (PubMed:33289480).
Plays a role in positively regulating fertility (PubMed:33283887).
May play a role in the translation of leucine and proline codons (Probable). May be required for the translation of specific mRNAs such as mRNAs involved in gonad development, collagen production and cuticle integrity (PubMed:33289480).
Plays a role in ensuring the correct localization of the germline-specific protein gld-1 during development (PubMed:33289480).
Not required for pre-rRNA processing, the production of mature 5S, 5.8S, 18S or 26S rRNAs or global translation (PubMed:33289480).
Plays a role in positively regulating fertility (PubMed:33283887).
Catalytic activity
- a cytidine in 26S rRNA + S-adenosyl-L-methionine = a 5-methylcytidine in 26S rRNA + H+ + S-adenosyl-L-homocysteine
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleolus | |
Molecular Function | RNA binding | |
Molecular Function | rRNA (cytosine-C5-)-methyltransferase activity | |
Biological Process | cuticle development | |
Biological Process | gonad morphogenesis | |
Biological Process | maturation of LSU-rRNA | |
Biological Process | negative regulation of cellular response to heat | |
Biological Process | negative regulation of locomotion involved in locomotory behavior | |
Biological Process | positive regulation of egg-laying behavior | |
Biological Process | positive regulation of fertilization | |
Biological Process | positive regulation of multicellular organism growth | |
Biological Process | positive regulation of translation | |
Biological Process | rRNA base methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name26S rRNA (cytosine-C(5))-methyltransferase nsun-1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ9TYV5
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
RNAi-mediated knockdown in L1 larvae results in sterility (PubMed:33283887).
RNAi-mediated knockdown in adults results in reduced egg-laying (PubMed:33289480).
RNAi-mediated knockdown from day 0 of adulthood or in the germline does not affect lifespan (PubMed:33289480).
RNAi-mediated knockdown in somatic tissues reduces lifespan by 10% and reduces body length and results in morphological defects in the gonad leading to the production of no oocytes (PubMed:33289480).
RNAi-mediated knockdown increases resistance to heat stress and increases the speed of locomotion (PubMed:33289480).
RNAi-mediated knockdown disrupts the localization of the germline-specific protein gld-1, results in collagen deposition and increases cuticle permeability (PubMed:33289480).
RNAi-mediated knockdown reduces the methylation of cytosine to 5-methylcytosine (m5C) in 26S rRNA (PubMed:33289480).
RNAi-mediated knockdown does not affect the methylation of cytosine 2381 to 5-methylcytosine (m5C2381) in 26S rRNA (PubMed:33289480).
RNAi-mediated knockdown in adults results in reduced egg-laying (PubMed:33289480).
RNAi-mediated knockdown from day 0 of adulthood or in the germline does not affect lifespan (PubMed:33289480).
RNAi-mediated knockdown in somatic tissues reduces lifespan by 10% and reduces body length and results in morphological defects in the gonad leading to the production of no oocytes (PubMed:33289480).
RNAi-mediated knockdown increases resistance to heat stress and increases the speed of locomotion (PubMed:33289480).
RNAi-mediated knockdown disrupts the localization of the germline-specific protein gld-1, results in collagen deposition and increases cuticle permeability (PubMed:33289480).
RNAi-mediated knockdown reduces the methylation of cytosine to 5-methylcytosine (m5C) in 26S rRNA (PubMed:33289480).
RNAi-mediated knockdown does not affect the methylation of cytosine 2381 to 5-methylcytosine (m5C2381) in 26S rRNA (PubMed:33289480).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 439 | In mj437; viable and produce viable progeny. Reduces body length. Increases the reduction in body length, and at 25 degrees Celsius there is a reduced number of progeny in a nsun-4 (mj457), nsun-2 (mj458) and nsun-5 (tm3898) mutant background. Abolishes methylation of carbon-5 cysteines and its metabolic derivative 2'-O-methyl-5-hydroxymethylcytosine in RNA in a nsun-4 (mj457), nsun-2 (mj458) and nsun-5 (tm3898) mutant background. | ||||
Sequence: C → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000452476 | 1-664 | 26S rRNA (cytosine-C5)-methyltransferase nsun-1 | |||
Sequence: MAIVKKKKVSAASKAATEKPEVKKAVKRPVAETEAVTPKKKKLVKKVKKSAKKAHEEEPIEQVEKLQLIDDDEDGLEGLSFPGSDDEDLRDDYSDDDSDAGDHLPIEKKSAALDKQKEKIIAEGEEELQLNIANQATFELPTVEEIENEMKSVPNLEIVKQRIADVIQVLGDFKNRRDPQKSRENYVEVLKKDLCSQYGYNDYLMGKFMDLFPNGAELLEFLEANDNPRPVTIRANSLKVKRRDLAKNLINRGMNVDPAADWTKVGLVVYDSQVPVGATPEYLAGHYMIQGLNSLLPVMALAPQPGDRVLDMCSAPGGKTSHIAALMKNSGVLFANDANFTRCRAIIGNLHRLGVNNTVVCNLGGEEFSKIRPNGFDRILLDAPCSGTGVIWKDQSVKTSKDSQDVQRRHTMQRQLILSALDSLDANSPNGGYLVYSTCSVLVEENEAVVNFLLERRHCELVPTGLSIGVDGYTRFRDYRFHPSLSMTKRYYPHVHNIDGFYVAKIKKLSNAKMSKQGVMEKEKEKAAQKSQNKKNKAEAEASESSDDEEEKKNGVEVNGQKKPAKKQQQKKQKADGDDSDDDNNAPMKNIGSGARANKKRRNQKKAAEKQAAVKEDDGFNTVGNVKRAKKPTQFKSKVPKRAAARTGAKSVKNRRKKMLAKQQ |
Proteomic databases
Expression
Developmental stage
Expression increases during the larval stages to adulthood.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-105 | Disordered | ||||
Sequence: MAIVKKKKVSAASKAATEKPEVKKAVKRPVAETEAVTPKKKKLVKKVKKSAKKAHEEEPIEQVEKLQLIDDDEDGLEGLSFPGSDDEDLRDDYSDDDSDAGDHLP | ||||||
Compositional bias | 11-40 | Basic and acidic residues | ||||
Sequence: AASKAATEKPEVKKAVKRPVAETEAVTPKK | ||||||
Compositional bias | 50-66 | Basic and acidic residues | ||||
Sequence: SAKKAHEEEPIEQVEKL | ||||||
Compositional bias | 67-81 | Acidic residues | ||||
Sequence: QLIDDDEDGLEGLSF | ||||||
Region | 513-664 | Disordered | ||||
Sequence: KMSKQGVMEKEKEKAAQKSQNKKNKAEAEASESSDDEEEKKNGVEVNGQKKPAKKQQQKKQKADGDDSDDDNNAPMKNIGSGARANKKRRNQKKAAEKQAAVKEDDGFNTVGNVKRAKKPTQFKSKVPKRAAARTGAKSVKNRRKKMLAKQQ | ||||||
Compositional bias | 517-560 | Basic and acidic residues | ||||
Sequence: QGVMEKEKEKAAQKSQNKKNKAEAEASESSDDEEEKKNGVEVNG | ||||||
Compositional bias | 570-585 | Basic and acidic residues | ||||
Sequence: QKKQKADGDDSDDDNN | ||||||
Compositional bias | 607-624 | Basic and acidic residues | ||||
Sequence: AAEKQAAVKEDDGFNTVG | ||||||
Compositional bias | 648-664 | Basic residues | ||||
Sequence: GAKSVKNRRKKMLAKQQ |
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q9TYV5-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Namea
- Length664
- Mass (Da)73,905
- Last updated2001-10-01 v2
- ChecksumB5D2B1C00C4DFE09
Q9TYV5-2
- Nameb
- Differences from canonical
- 1-253: Missing
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_061010 | 1-253 | in isoform b | |||
Sequence: Missing | ||||||
Compositional bias | 11-40 | Basic and acidic residues | ||||
Sequence: AASKAATEKPEVKKAVKRPVAETEAVTPKK | ||||||
Compositional bias | 50-66 | Basic and acidic residues | ||||
Sequence: SAKKAHEEEPIEQVEKL | ||||||
Compositional bias | 67-81 | Acidic residues | ||||
Sequence: QLIDDDEDGLEGLSF | ||||||
Compositional bias | 517-560 | Basic and acidic residues | ||||
Sequence: QGVMEKEKEKAAQKSQNKKNKAEAEASESSDDEEEKKNGVEVNG | ||||||
Compositional bias | 570-585 | Basic and acidic residues | ||||
Sequence: QKKQKADGDDSDDDNN | ||||||
Compositional bias | 607-624 | Basic and acidic residues | ||||
Sequence: AAEKQAAVKEDDGFNTVG | ||||||
Compositional bias | 648-664 | Basic residues | ||||
Sequence: GAKSVKNRRKKMLAKQQ |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX284602 EMBL· GenBank· DDBJ | CCD73642.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284602 EMBL· GenBank· DDBJ | CUR29991.1 EMBL· GenBank· DDBJ | Genomic DNA |