Q9TYV5 · NSUN1_CAEEL

  • Protein
    26S rRNA (cytosine-C(5))-methyltransferase nsun-1
  • Gene
    nsun-1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Methyltransferase which methylates the carbon-5 position of cytosine 2982 to 5-methylcytosine (m5C2982) in 26S rRNA (PubMed:33283887, PubMed:33289480).
May play a role in the translation of leucine and proline codons (Probable). May be required for the translation of specific mRNAs such as mRNAs involved in gonad development, collagen production and cuticle integrity (PubMed:33289480).
Plays a role in ensuring the correct localization of the germline-specific protein gld-1 during development (PubMed:33289480).
Not required for pre-rRNA processing, the production of mature 5S, 5.8S, 18S or 26S rRNAs or global translation (PubMed:33289480).
Plays a role in positively regulating fertility (PubMed:33283887).

Catalytic activity

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site313-319S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site337S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site382S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site439Nucleophile

GO annotations

AspectTerm
Cellular Componentnucleolus
Molecular FunctionRNA binding
Molecular FunctionrRNA (cytosine-C5-)-methyltransferase activity
Biological Processcuticle development
Biological Processgonad morphogenesis
Biological Processmaturation of LSU-rRNA
Biological Processnegative regulation of cellular response to heat
Biological Processnegative regulation of locomotion involved in locomotory behavior
Biological Processpositive regulation of egg-laying behavior
Biological Processpositive regulation of fertilization
Biological Processpositive regulation of multicellular organism growth
Biological Processpositive regulation of translation
Biological ProcessrRNA base methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    26S rRNA (cytosine-C(5))-methyltransferase nsun-1
  • EC number
  • Alternative names
    • 5-methylcytosine rRNA methyltransferase nsun-1
    • NOL1/NOP2/Sun domain family member 1
    • RNA cytosine C(5)-methyltransferase nsun-1
    • rRNA cytosine C(5)-methyltransferase nsun-1

Gene names

    • Name
      nsun-1
    • Synonyms
      nol-1
      , nol-2
    • ORF names
      W07E6.1

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    Q9TYV5
  • Secondary accessions
    • A0A0M7RDQ9

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

RNAi-mediated knockdown in L1 larvae results in sterility (PubMed:33283887).
RNAi-mediated knockdown in adults results in reduced egg-laying (PubMed:33289480).
RNAi-mediated knockdown from day 0 of adulthood or in the germline does not affect lifespan (PubMed:33289480).
RNAi-mediated knockdown in somatic tissues reduces lifespan by 10% and reduces body length and results in morphological defects in the gonad leading to the production of no oocytes (PubMed:33289480).
RNAi-mediated knockdown increases resistance to heat stress and increases the speed of locomotion (PubMed:33289480).
RNAi-mediated knockdown disrupts the localization of the germline-specific protein gld-1, results in collagen deposition and increases cuticle permeability (PubMed:33289480).
RNAi-mediated knockdown reduces the methylation of cytosine to 5-methylcytosine (m5C) in 26S rRNA (PubMed:33289480).
RNAi-mediated knockdown does not affect the methylation of cytosine 2381 to 5-methylcytosine (m5C2381) in 26S rRNA (PubMed:33289480).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis439In mj437; viable and produce viable progeny. Reduces body length. Increases the reduction in body length, and at 25 degrees Celsius there is a reduced number of progeny in a nsun-4 (mj457), nsun-2 (mj458) and nsun-5 (tm3898) mutant background. Abolishes methylation of carbon-5 cysteines and its metabolic derivative 2'-O-methyl-5-hydroxymethylcytosine in RNA in a nsun-4 (mj457), nsun-2 (mj458) and nsun-5 (tm3898) mutant background.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004524761-66426S rRNA (cytosine-C5)-methyltransferase nsun-1

Proteomic databases

Expression

Developmental stage

Expression increases during the larval stages to adulthood.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region1-105Disordered
Compositional bias11-40Basic and acidic residues
Compositional bias50-66Basic and acidic residues
Compositional bias67-81Acidic residues
Region513-664Disordered
Compositional bias517-560Basic and acidic residues
Compositional bias570-585Basic and acidic residues
Compositional bias607-624Basic and acidic residues
Compositional bias648-664Basic residues

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q9TYV5-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    664
  • Mass (Da)
    73,905
  • Last updated
    2001-10-01 v2
  • Checksum
    B5D2B1C00C4DFE09
MAIVKKKKVSAASKAATEKPEVKKAVKRPVAETEAVTPKKKKLVKKVKKSAKKAHEEEPIEQVEKLQLIDDDEDGLEGLSFPGSDDEDLRDDYSDDDSDAGDHLPIEKKSAALDKQKEKIIAEGEEELQLNIANQATFELPTVEEIENEMKSVPNLEIVKQRIADVIQVLGDFKNRRDPQKSRENYVEVLKKDLCSQYGYNDYLMGKFMDLFPNGAELLEFLEANDNPRPVTIRANSLKVKRRDLAKNLINRGMNVDPAADWTKVGLVVYDSQVPVGATPEYLAGHYMIQGLNSLLPVMALAPQPGDRVLDMCSAPGGKTSHIAALMKNSGVLFANDANFTRCRAIIGNLHRLGVNNTVVCNLGGEEFSKIRPNGFDRILLDAPCSGTGVIWKDQSVKTSKDSQDVQRRHTMQRQLILSALDSLDANSPNGGYLVYSTCSVLVEENEAVVNFLLERRHCELVPTGLSIGVDGYTRFRDYRFHPSLSMTKRYYPHVHNIDGFYVAKIKKLSNAKMSKQGVMEKEKEKAAQKSQNKKNKAEAEASESSDDEEEKKNGVEVNGQKKPAKKQQQKKQKADGDDSDDDNNAPMKNIGSGARANKKRRNQKKAAEKQAAVKEDDGFNTVGNVKRAKKPTQFKSKVPKRAAARTGAKSVKNRRKKMLAKQQ

Q9TYV5-2

  • Name
    b
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Features

Showing features for alternative sequence, compositional bias.

TypeIDPosition(s)Description
Alternative sequenceVSP_0610101-253in isoform b
Compositional bias11-40Basic and acidic residues
Compositional bias50-66Basic and acidic residues
Compositional bias67-81Acidic residues
Compositional bias517-560Basic and acidic residues
Compositional bias570-585Basic and acidic residues
Compositional bias607-624Basic and acidic residues
Compositional bias648-664Basic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BX284602
EMBL· GenBank· DDBJ
CCD73642.1
EMBL· GenBank· DDBJ
Genomic DNA
BX284602
EMBL· GenBank· DDBJ
CUR29991.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp