Q9TTA5 · SMAL1_BOVIN

  • Protein
    SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
  • Gene
    SMARCAL1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks (By similarity).

Features

Showing features for binding site.

1940100200300400500600700800900
TypeIDPosition(s)Description
Binding site455-462ATP (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentnuclear replication fork
Cellular Componentnucleus
Cellular Componentsite of double-strand break
Molecular FunctionATP binding
Molecular FunctionATP-dependent chromatin remodeler activity
Molecular FunctionATP-dependent DNA/DNA annealing activity
Molecular Functionhelicase activity
Molecular Functionhydrolase activity
Biological ProcessDNA damage response
Biological ProcessDNA repair
Biological Processregulation of transcription by RNA polymerase II
Biological Processreplication fork processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
  • EC number
  • Alternative names
    • HepA-related protein
    • Sucrose nonfermenting protein 2-like 1

Gene names

    • Name
      SMARCAL1
    • Synonyms
      HARP

Organism names

  • Taxonomic identifier
  • Strain
    • Hereford
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos

Accessions

  • Primary accession
    Q9TTA5
  • Secondary accessions
    • F1MDP3

Proteomes

Subcellular Location

Nucleus
Note: Recruited to damaged DNA regions.

Keywords

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain.

Type
IDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylserine
ChainPRO_00000743472-940SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
Modified residue125Phosphoserine
Modified residue131Phosphoserine
Modified residue153Phosphoserine
Modified residue200Phosphoserine

Post-translational modification

DNA damage-regulated phosphorylation by kinases that may include ATM, ATR and PRKDC.

Keywords

Proteomic databases

Expression

Tissue specificity

Expressed in mature oocytes, 2-4 cell stage embryos and 8-16 cell stage embryos. Expressed at lower levels in morulae and blastocysts.

Developmental stage

Expressed during early embryogenesis.

Interaction

Subunit

Interacts with RPA2; the interaction is direct and mediates the recruitment by the RPA complex of SMARCAL1 to sites of DNA damage.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias, coiled coil, domain, motif.

Type
IDPosition(s)Description
Region1-155Disordered
Region2-36Mediates interaction with RPA2
Compositional bias18-39Basic and acidic residues
Coiled coil18-40
Compositional bias40-98Polar residues
Domain229-299HARP 1
Domain325-396HARP 2
Domain442-597Helicase ATP-binding
Motif546-549DESH box
Region662-682Disordered
Domain708-864Helicase C-terminal
Region899-918Disordered

Sequence similarities

Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    940
  • Mass (Da)
    104,776
  • Last updated
    2014-07-09 v2
  • Checksum
    AB29A63444B23C95
MSISPLKCPCLLQRSRGKIEANRQKALARRAEKLLAEQHQKPAQSKQGPSQNLPRDPSKSGSHGIFFKQQNPSSSSHGDQRPQNPHSFSPNTSEQAKGMWQRPEEMPTACPSYRPPNQVTVAGISPPLANSPPGVPSQQLWGCELGQGHPQASLETQSTPFANTTHEPLAKVKNFQETAASSCGQPPRDPELEARMARPSTSGQNISGSVMPRTEGRLQQKAGTPLHRVVGSQQGRCIRNGERFQVKIGYNEALIAVFKSLPSRSYDPATKTWNFSMTDYGPLMKAAQRLPGITLQPLEGAEGHMESPSTSSGIIAKTGLPAAPSLAFVKGQCVLISRARFEADISYSEDLIALFKQMDSRKYDVKTRKWSFLLEEYSKLMERVRGLPQVQLDPLPKTLTLFRAQLQKTSLSPVADIPEADLSRVDSKLVSSLLPFQRAGVNFAIAQRGRLLLADDMGLGKTIQAICIAAYYRKEWPLLVVVPSSVRFTWEQAFCRWLPSLNPLDINVVVTGKDRLTDGLVNIVSFDLLSKLEKQLKPPFKVVIIDESHFLKNIKTARCRAAMPLLKVAKRVILLSGTPAMSRPAELYTQILAVRPTFFPQFHAFGLRYCGAKRQPWGWDYSGSSNLGELKLLLEEAVMLRRLKGDVLSQLPAKQARWWWSPQARSTPGPEPPWMPPPRMTTKDKTKQQQKEALILFFNRTAEAKIPSIIEYILDLLESGREKFLVFAHHKVVLDAITKELERKRVQHIRIDGSTSSADRETSASSFSCPRALRGVLSITAANMGLTFSSADLVVFGELFWNPGVLMQAEDRVHRIGQLSSVSIHYLVARGTADDYLWPLIQEKIKVLGEAGLSETNFSEMTEATDYFSKDSKQQKIYNLFQKSFEEDGNDMELLEAAESFDPGSQDTGDKLDESTLTGSPVKKKRFEFFDNWDSFTSPL

Features

Showing features for sequence conflict, compositional bias.

Type
IDPosition(s)Description
Sequence conflict17in Ref. 1; AAF22285
Compositional bias18-39Basic and acidic residues
Compositional bias40-98Polar residues
Sequence conflict89in Ref. 1; AAF22285
Sequence conflict126in Ref. 1; AAF22285
Sequence conflict162in Ref. 1; AAF22285
Sequence conflict170in Ref. 1; AAF22285
Sequence conflict196-197in Ref. 1; AAF22285
Sequence conflict387in Ref. 1; AAF22285
Sequence conflict558-560in Ref. 1; AAF22285
Sequence conflict656in Ref. 1; AAF22285
Sequence conflict667in Ref. 1; AAF22285
Sequence conflict678-679in Ref. 1; AAF22285

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF173643
EMBL· GenBank· DDBJ
AAF22285.1
EMBL· GenBank· DDBJ
mRNA
DAAA02005900
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

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