Q9TTA5 · SMAL1_BOVIN
- ProteinSWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
- GeneSMARCAL1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids940 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks (By similarity).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 455-462 | ATP (UniProtKB | ChEBI) | |||
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nuclear replication fork | |
Cellular Component | nucleus | |
Cellular Component | site of double-strand break | |
Molecular Function | ATP binding | |
Molecular Function | ATP-dependent chromatin remodeler activity | |
Molecular Function | ATP-dependent DNA/DNA annealing activity | |
Molecular Function | helicase activity | |
Molecular Function | hydrolase activity | |
Biological Process | DNA damage response | |
Biological Process | DNA repair | |
Biological Process | regulation of transcription by RNA polymerase II | |
Biological Process | replication fork processing |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos
Accessions
- Primary accessionQ9TTA5
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Recruited to damaged DNA regions.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Initiator methionine | 1 | Removed | |||
Modified residue | 2 | N-acetylserine | |||
Chain | PRO_0000074347 | 2-940 | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 | ||
Modified residue | 125 | Phosphoserine | |||
Modified residue | 131 | Phosphoserine | |||
Modified residue | 153 | Phosphoserine | |||
Modified residue | 200 | Phosphoserine | |||
Post-translational modification
DNA damage-regulated phosphorylation by kinases that may include ATM, ATR and PRKDC.
Keywords
- PTM
Proteomic databases
Expression
Tissue specificity
Expressed in mature oocytes, 2-4 cell stage embryos and 8-16 cell stage embryos. Expressed at lower levels in morulae and blastocysts.
Developmental stage
Expressed during early embryogenesis.
Interaction
Subunit
Interacts with RPA2; the interaction is direct and mediates the recruitment by the RPA complex of SMARCAL1 to sites of DNA damage.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, coiled coil, domain, motif.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-155 | Disordered | |||
Region | 2-36 | Mediates interaction with RPA2 | |||
Compositional bias | 18-39 | Basic and acidic residues | |||
Coiled coil | 18-40 | ||||
Compositional bias | 40-98 | Polar residues | |||
Domain | 229-299 | HARP 1 | |||
Domain | 325-396 | HARP 2 | |||
Domain | 442-597 | Helicase ATP-binding | |||
Motif | 546-549 | DESH box | |||
Region | 662-682 | Disordered | |||
Domain | 708-864 | Helicase C-terminal | |||
Region | 899-918 | Disordered | |||
Sequence similarities
Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length940
- Mass (Da)104,776
- Last updated2014-07-09 v2
- ChecksumAB29A63444B23C95
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 17 | in Ref. 1; AAF22285 | |||
Compositional bias | 18-39 | Basic and acidic residues | |||
Compositional bias | 40-98 | Polar residues | |||
Sequence conflict | 89 | in Ref. 1; AAF22285 | |||
Sequence conflict | 126 | in Ref. 1; AAF22285 | |||
Sequence conflict | 162 | in Ref. 1; AAF22285 | |||
Sequence conflict | 170 | in Ref. 1; AAF22285 | |||
Sequence conflict | 196-197 | in Ref. 1; AAF22285 | |||
Sequence conflict | 387 | in Ref. 1; AAF22285 | |||
Sequence conflict | 558-560 | in Ref. 1; AAF22285 | |||
Sequence conflict | 656 | in Ref. 1; AAF22285 | |||
Sequence conflict | 667 | in Ref. 1; AAF22285 | |||
Sequence conflict | 678-679 | in Ref. 1; AAF22285 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF173643 EMBL· GenBank· DDBJ | AAF22285.1 EMBL· GenBank· DDBJ | mRNA | ||
DAAA02005900 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |