Q9TME7 · Q9TME7_9PHAE
- ProteinRibulose bisphosphate carboxylase large chain
- GenerbcL
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids488 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.
Miscellaneous
The basic functional RuBisCO is composed of a large chain homodimer in a 'head-to-tail' conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric 'cap' on each end of the 'barrel'.
Catalytic activity
- 2 (2R)-3-phosphoglycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O
- D-ribulose 1,5-bisphosphate + O2 = 2-phosphoglycolate + (2R)-3-phosphoglycerate + 2 H+
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 127 | substrate; in homodimeric partner | |||
Binding site | 177 | substrate | |||
Active site | 179 | Proton acceptor | |||
Binding site | 181 | substrate | |||
Binding site | 205 | Mg2+ (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 207 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 208 | Mg2+ (UniProtKB | ChEBI) | |||
Active site | 297 | Proton acceptor | |||
Binding site | 298 | substrate | |||
Binding site | 330 | substrate | |||
Site | 337 | Transition state stabilizer | |||
Binding site | 382 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | membrane | |
Molecular Function | magnesium ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | ribulose-bisphosphate carboxylase activity | |
Biological Process | reductive pentose-phosphate cycle |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibulose bisphosphate carboxylase large chain
- EC number
- Short namesRuBisCO large subunit
Gene names
Encoded on
- Plastid
Organism names
- Organism
- Taxonomic lineageEukaryota > Sar > Stramenopiles > Ochrophyta > PX clade > Phaeophyceae > Ectocarpales > Scytosiphonaceae > Endarachne
Accessions
- Primary accessionQ9TME7
Subcellular Location
UniProt Annotation
GO Annotation
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 260-280 | Helical | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 205 | N6-carboxylysine | |||
Interaction
Subunit
Heterohexadecamer of 8 large chains and 8 small chains.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 28-148 | Ribulose bisphosphate carboxylase large subunit ferrodoxin-like N-terminal | |||
Domain | 158-465 | Ribulose bisphosphate carboxylase large subunit C-terminal | |||
Sequence similarities
Belongs to the RuBisCO large chain family. Type I subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length488
- Mass (Da)53,967
- Last updated2000-05-01 v1
- ChecksumE441E0E64536349B
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
MF460360 EMBL· GenBank· DDBJ | AXC47209.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB022244 EMBL· GenBank· DDBJ | BAA86087.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB578992 EMBL· GenBank· DDBJ | BAJ78329.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB578993 EMBL· GenBank· DDBJ | BAJ78330.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB860185 EMBL· GenBank· DDBJ | BAO56985.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB860186 EMBL· GenBank· DDBJ | BAO56986.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB871992 EMBL· GenBank· DDBJ | BAO56994.1 EMBL· GenBank· DDBJ | Genomic DNA |