Q9T0N8 · CKX1_MAIZE

  • Protein
    Cytokinin dehydrogenase 1
  • Gene
    CKX1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the oxidation of cytokinins, a family of N6-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group (PubMed:11154345).
Cleaves trans-zeatin, N6-dimethylallyladenine (isopentenyladenine), isopentenyladenosine, zeatin riboside and cis-zeatin, but not dihydrozeatin, kinetin and benzylaminopurine (PubMed:11154345).

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Activity regulation

Competitive inhibition by phenylureas.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site100FAD (UniProtKB | ChEBI)
Binding site102FAD (UniProtKB | ChEBI)
Binding site103FAD (UniProtKB | ChEBI)
Binding site104FAD (UniProtKB | ChEBI)
Binding site106FAD (UniProtKB | ChEBI)
Binding site110FAD (UniProtKB | ChEBI)
Binding site169FAD (UniProtKB | ChEBI)
Binding site169N6-dimethylallyladenine (UniProtKB | ChEBI)
Binding site169trans-zeatin (UniProtKB | ChEBI)
Binding site174FAD (UniProtKB | ChEBI)
Binding site180FAD (UniProtKB | ChEBI)
Binding site184FAD (UniProtKB | ChEBI)
Binding site235FAD (UniProtKB | ChEBI)
Binding site381N6-dimethylallyladenine (UniProtKB | ChEBI)
Binding site381trans-zeatin (UniProtKB | ChEBI)
Binding site456trans-zeatin (UniProtKB | ChEBI)
Binding site491FAD (UniProtKB | ChEBI)
Binding site527FAD (UniProtKB | ChEBI)
Binding site530FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentextracellular region
Molecular Functioncytokinin dehydrogenase activity
Molecular FunctionFAD binding
Molecular Functionoxidoreductase activity
Biological Processcytokinin metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytokinin dehydrogenase 1
  • EC number
  • Alternative names
    • Cytokinin oxidase 1 (CKO 1; COX 1)
    • ZmCKX1

Gene names

    • Name
      CKX1

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Nobilis
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > PACMAD clade > Panicoideae > Andropogonodae > Andropogoneae > Tripsacinae > Zea

Accessions

  • Primary accession
    Q9T0N8
  • Secondary accessions
    • O81158

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

PTM/Processing

Features

Showing features for signal, chain, glycosylation, modified residue.

TypeIDPosition(s)Description
Signal1-18
ChainPRO_000002042419-534Cytokinin dehydrogenase 1
Glycosylation52N-linked (GlcNAc...) asparagine
Glycosylation63N-linked (GlcNAc...) asparagine
Glycosylation89N-linked (GlcNAc...) asparagine
Modified residue105Pros-8alpha-FAD histidine
Glycosylation134N-linked (GlcNAc...) asparagine
Glycosylation294N-linked (GlcNAc...) asparagine
Glycosylation323N-linked (GlcNAc...) asparagine
Glycosylation338N-linked (GlcNAc...) asparagine
Glycosylation434N-linked (GlcNAc...) asparagine

Post-translational modification

Glycosylated; with approximately 10 hexose residues per site.

Keywords

PTM databases

Expression

Tissue specificity

Expressed in immature kernels and unpollinated cobs. Weakly expressed in kernels harvested two weeks after anthesis.

Gene expression databases

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain65-245FAD-binding PCMH-type

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    534
  • Mass (Da)
    57,229
  • Last updated
    2005-05-24 v2
  • Checksum
    13FB5AF654C169E5
MAVVYYLLLAGLIACSHALAAGTPALGDDRGRPWPASLAALALDGKLRTDSNATAAASTDFGNITSALPAAVLYPSSTGDLVALLSAANSTPGWPYTIAFRGRGHSLMGQAFAPGGVVVNMASLGDAAAPPRINVSADGRYVDAGGEQVWIDVLRASLARGVAPRSWNDYLYLTVGGTLSNAGISGQAFRHGPQISNVLEMDVITGHGEMVTCSKQLNADLFDAVLGGLGQFGVITRARIAVEPAPARARWVRFVYTDFAAFSADQERLTAPRPGGGGASFGPMSYVEGSVFVNQSLATDLANTGFFTDADVARIVALAGERNATTVYSIEATLNYDNATAAAAAVDQELASVLGTLSYVEGFAFQRDVAYAAFLDRVHGEEVALNKLGLWRVPHPWLNMFVPRSRIADFDRGVFKGILQGTDIVGPLIVYPLNKSMWDDGMSAATPSEDVFYAVSLLFSSVAPNDLARLQEQNRRILRFCDLAGIQYKTYLARHTDRSDWVRHFGAAKWNRFVEMKNKYDPKRLLSPGQDIFN

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict79in Ref. 1; CAA77151
Sequence conflict168in Ref. 1; CAA77151
Sequence conflict254in Ref. 1; CAA77151

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Y18377
EMBL· GenBank· DDBJ
CAA77151.1
EMBL· GenBank· DDBJ
mRNA
AF044603
EMBL· GenBank· DDBJ
AAC27500.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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