Q9SZM3 · CDPKQ_ARATH
- ProteinCalcium-dependent protein kinase 26
- GeneCPK26
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids484 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
May play a role in signal transduction pathways that involve calcium as a second messenger.
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]
Activity regulation
Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 30-38 | ATP (UniProtKB | ChEBI) | ||||
Sequence: LGQGQFGTT | ||||||
Binding site | 53 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Active site | 148 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 338 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 340 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 342 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 349 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 374 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 376 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 378 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 380 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 385 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 410 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 412 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 414 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 416 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 421 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 444 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 446 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 448 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 450 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 455 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | calcium ion binding | |
Molecular Function | calcium-dependent protein serine/threonine kinase activity | |
Molecular Function | calmodulin binding | |
Molecular Function | calmodulin-dependent protein kinase activity | |
Molecular Function | protein serine kinase activity | |
Biological Process | intracellular signal transduction |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCalcium-dependent protein kinase 26
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9SZM3
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000363348 | 1-484 | Calcium-dependent protein kinase 26 | |||
Sequence: MKHSGGNQACYVLGQKTPSIRDLYSLGHKLGQGQFGTTYMCKEISTGREYACKSITKRKLISKEDVEDVRREIQIMHHLAGYKNIVTIKGAYEDPLYVHIVMELCSGGELFDRIIQRGHYSERKAAELIKIIVGVVEACHSLGVMHRDLKPENFLLVNKDDDFSLKAIDFGLSVFFKPGQIFEDVVGSPYYVAPEVLLKHYGPEADVWTAGVILYILVSGVPPFWAETQQGIFDAVLKGHIDFDSDPWPLISDSAKNLIRGMLCSRPSERLTAHQVLRHPWICENGVAPDRALDPAVLSRLKQFSAMNKLKQMALRVIAESLSEEEIAGLKEMFKAMDTDNSGAITFDELKAGLRRYGSTLKDTEIRDLMEAADIDKSGTIDYGEFIAATIHLNKLEREEHLLSAFRYFDKDGSGYITIDELQHACAEQGMSDVFLEDVIKEVDQDNDGRIDYGEFVAMMQKGIVGRTMRKSINMSIRNNAVSQ | ||||||
Modified residue | 188 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 24-282 | Protein kinase | ||||
Sequence: YSLGHKLGQGQFGTTYMCKEISTGREYACKSITKRKLISKEDVEDVRREIQIMHHLAGYKNIVTIKGAYEDPLYVHIVMELCSGGELFDRIIQRGHYSERKAAELIKIIVGVVEACHSLGVMHRDLKPENFLLVNKDDDFSLKAIDFGLSVFFKPGQIFEDVVGSPYYVAPEVLLKHYGPEADVWTAGVILYILVSGVPPFWAETQQGIFDAVLKGHIDFDSDPWPLISDSAKNLIRGMLCSRPSERLTAHQVLRHPWI | ||||||
Region | 288-318 | Autoinhibitory domain | ||||
Sequence: APDRALDPAVLSRLKQFSAMNKLKQMALRVI | ||||||
Domain | 325-360 | EF-hand 1 | ||||
Sequence: EEIAGLKEMFKAMDTDNSGAITFDELKAGLRRYGST | ||||||
Domain | 361-396 | EF-hand 2 | ||||
Sequence: LKDTEIRDLMEAADIDKSGTIDYGEFIAATIHLNKL | ||||||
Domain | 397-432 | EF-hand 3 | ||||
Sequence: EREEHLLSAFRYFDKDGSGYITIDELQHACAEQGMS | ||||||
Domain | 436-466 | EF-hand 4 | ||||
Sequence: LEDVIKEVDQDNDGRIDYGEFVAMMQKGIVG |
Domain
There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (288-318) inactivates kinase activity under calcium-free conditions (By similarity).
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q9SZM3-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length484
- Mass (Da)54,343
- Last updated2000-05-01 v1
- Checksum6D7299927264FD38
Q9SZM3-2
- Name2
- Differences from canonical
- 1-144: Missing
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F4JTL3 | F4JTL3_ARATH | CPK26 | 514 |
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_036291 | 1-144 | in isoform 2 | |||
Sequence: Missing | ||||||
Sequence conflict | 249 | in Ref. 3; BX827943 | ||||
Sequence: P → A |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL035538 EMBL· GenBank· DDBJ | CAB37563.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL161593 EMBL· GenBank· DDBJ | CAB80488.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | AEE86899.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | AEE86901.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | ANM67055.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX827943 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
BT029228 EMBL· GenBank· DDBJ | ABJ98560.1 EMBL· GenBank· DDBJ | mRNA |