Q9SYM5 · RHM1_ARATH
- ProteinTrifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM1
- GeneRHM1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids669 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Trifunctional enzyme involved in UDP-beta-L-rhamnose biosynthesis, a precursor of the primary cell wall components rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II) (PubMed:14671019, PubMed:17190829, PubMed:19056285).
Plays a major role in supplying UDP-rhamnose for flavonol biosynthesis (PubMed:18757557).
Catalyzes the dehydration of UDP-glucose to form UDP-4-dehydro-6-deoxy-D-glucose followed by the epimerization of the C3' and C5' positions of UDP-4-dehydro-6-deoxy-D-glucose to form UDP-4-keto-beta-L-rhamnose and the reduction of UDP-4-keto-beta-L-rhamnose to yield UDP-beta-L-rhamnose (By similarity).
Plays a major role in supplying UDP-rhamnose for flavonol biosynthesis (PubMed:18757557).
Catalyzes the dehydration of UDP-glucose to form UDP-4-dehydro-6-deoxy-D-glucose followed by the epimerization of the C3' and C5' positions of UDP-4-dehydro-6-deoxy-D-glucose to form UDP-4-keto-beta-L-rhamnose and the reduction of UDP-4-keto-beta-L-rhamnose to yield UDP-beta-L-rhamnose (By similarity).
Miscellaneous
The increased accumulation of auxin in rol1-2 seedlings appears to be caused by a flavonol-induced modification of auxin transport (PubMed:18567791, PubMed:21502189).
In bacteria, TDP-L-rhamnose is formed by the successive action of three different enzymes on TDP-D-glucose. In plants, on the other hand, a single polypeptide probably catalyzes all three reactions that lead to the conversion of UDP-D-glucose to UDP-L-rhamnose
In bacteria, TDP-L-rhamnose is formed by the successive action of three different enzymes on TDP-D-glucose. In plants, on the other hand, a single polypeptide probably catalyzes all three reactions that lead to the conversion of UDP-D-glucose to UDP-L-rhamnose
Catalytic activity
- UDP-alpha-D-glucose = H2O + UDP-4-dehydro-6-deoxy-alpha-D-glucose
Cofactor
Protein has several cofactor binding sites:
Pathway
Carbohydrate biosynthesis.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | mitochondrion | |
Cellular Component | plasmodesma | |
Molecular Function | dTDP-glucose 4,6-dehydratase activity | |
Molecular Function | isomerase activity | |
Molecular Function | oxidoreductase activity | |
Molecular Function | UDP-glucose 4,6-dehydratase activity | |
Molecular Function | UDP-L-rhamnose synthase activity | |
Biological Process | auxin export across the plasma membrane | |
Biological Process | cell differentiation | |
Biological Process | cell wall organization | |
Biological Process | flavonol biosynthetic process | |
Biological Process | regulation of cell population proliferation | |
Biological Process | UDP-rhamnose biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTrifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM1
- Alternative names
Including 2 domains:
- Recommended nameUDP-glucose 4,6-dehydratase
- EC number
- Recommended nameUDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose 4-keto-reductase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9SYM5
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Hyponastic growth, aberrant pavement cell and stomatal morphology in cotyledons, and defective trichome formation.
Features
Showing features for mutagenesis.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 17 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000183252 | 1-669 | Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM1 | |||
Sequence: MASYTPKNILITGAAGFIASHVANRLIRSYPDYKIVVLDKLDYCSNLKNLNPSKHSPNFKFVKGDIASADLVNHLLITEGIDTIMHFAAQTHVDNSFGNSFEFTKNNIYGTHVLLEACKVTGQIRRFIHVSTDEVYGETDEDALVGNHEASQLLPTNPYSATKAGAEMLVMAYGRSYGLPVITTRGNNVYGPNQFPEKLIPKFILLAMRGQVLPIHGDGSNVRSYLYCEDVAEAFEVVLHKGEVGHVYNIGTKKERRVNDVAKDICKLFNMDPEANIKFVDNRPFNDQRYFLDDQKLKKLGWSERTTWEEGLKKTMDWYTQNPEWWGDVSGALLPHPRMLMMPGGRHFDGSEDNSLAATLSEKPSQTHMVVPSQRSNGTPQKPSLKFLIYGKTGWIGGLLGKICDKQGIAYEYGKGRLEDRSSLLQDIQSVKPTHVFNSAGVTGRPNVDWCESHKTETIRANVAGTLTLADVCREHGLLMMNFATGCIFEYDDKHPEGSGIGFKEEDTPNFTGSFYSKTKAMVEELLKEYDNVCTLRVRMPISSDLNNPRNFITKISRYNKVVNIPNSMTVLDELLPISIEMAKRNLKGIWNFTNPGVVSHNEILEMYRDYINPEFKWANFTLEEQAKVIVAPRSNNEMDASKLKKEFPELLSIKESLIKYAYGPNKKT |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in roots, stems, leaves, seedlings, inflorescence tips, and siliques. Detected in the adaxial side of cotyledons, in the emerging leaves and in trichomes. Also detected in the root tip, more precisely in the epidermal cells in the meristematic and elongation zone.
Gene expression databases
Structure
Family & Domains
Domain
The dehydratase activity is contained in the N-terminal region while the epimerase and reductase activities are in the C-terminal region.
Sequence similarities
In the N-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.
In the C-terminal section; belongs to the dTDP-4-dehydrorhamnose reductase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length669
- Mass (Da)75,372
- Last updated2000-05-01 v1
- ChecksumFB8DF9C864F176D4
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC007260 EMBL· GenBank· DDBJ | AAD30579.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE36122.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY042833 EMBL· GenBank· DDBJ | AAK68773.1 EMBL· GenBank· DDBJ | mRNA | ||
AY081471 EMBL· GenBank· DDBJ | AAM10033.1 EMBL· GenBank· DDBJ | mRNA |