Q9SXS3 · C93C2_GLYEC
- Protein2-hydroxyisoflavanone synthase
- GeneCYP93C2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids523 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
2-hydroxyisoflavanone synthase, which catalyzes the hydroxylation associated with 1,2-aryl migration of flavanones. Converts liquiritigenin and naringenin into highly unstable precursors of the isoflavones daidzein and genistein. Acts only on substrates with (2S)-chirality.
Miscellaneous
Lys-375 is essential for the intramolecular aryl migration of a flavanone molecule to produce the isoflavonoid skeleton (PubMed:12207646).
Arg-104 and Leu-371 are essential for the correct P450 conformation (PubMed:12207646, PubMed:15809082).
Arg-104 and Leu-371 are essential for the correct P450 conformation (PubMed:12207646, PubMed:15809082).
Catalytic activity
- (2S)-liquiritigenin + reduced [NADPH--hemoprotein reductase] + O2 = (2R,3S)-2,4',7-trihydroxyisoflavanone + oxidized [NADPH--hemoprotein reductase] + H2O + H+
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
8 μM | liquiritigenin |
pH Dependence
Optimum pH is 7.5.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | intracellular membrane-bounded organelle | |
Cellular Component | membrane | |
Molecular Function | 2-hydroxyisoflavanone synthase activity | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen | |
Biological Process | isoflavonoid biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-hydroxyisoflavanone synthase
- EC number
- Short names2HI synthase
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > Hologalegina > IRL clade > Galegeae > Glycyrrhiza
Accessions
- Primary accessionQ9SXS3
Subcellular Location
UniProt Annotation
GO Annotation
Microsome membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 2-22 | Helical | ||||
Sequence: LVELAITLLVIALFIHLRPTL |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 104 | Total loss of catalytic activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 106 | No effect on catalytic activity. | ||||
Sequence: Q → E | ||||||
Mutagenesis | 119 | No effect on catalytic activity. | ||||
Sequence: V → L | ||||||
Mutagenesis | 305 | No effect on catalytic activity. | ||||
Sequence: S → T | ||||||
Mutagenesis | 310 | Decreased intramolecular 1,2-aryl migration and increased production of 3-hydroxyflavanone and flavone. | ||||
Sequence: S → A or V | ||||||
Mutagenesis | 310 | Decreased intramolecular 1,2-aryl migration and increased production of 3-hydroxyflavanone and flavone. 5% activity and production of mainly 3-hydroxyflavanone; when associated with T-375. 0.1% activity and production of only flavone; when associated with V-371 and T-375. | ||||
Sequence: S → T | ||||||
Mutagenesis | 371 | Production of mainly 3-hydroxyflavanone; when associated with T-375. | ||||
Sequence: L → I | ||||||
Mutagenesis | 371 | Total loss of catalytic activity. 5% activity and production of mainly 3-hydroxyflavanone; when associated with T-375. 0.1% activity and production of only flavone; when associated with T-310 and T-375. | ||||
Sequence: L → V | ||||||
Mutagenesis | 375 | Loss of intramolecular 1,2-aryl migration and production of only 3-hydroxyflavanone. | ||||
Sequence: K → A or R | ||||||
Mutagenesis | 375 | Loss of intramolecular 1,2-aryl migration and production of only 3-hydroxyflavanone. 5% activity and production of mainly 3-hydroxyflavanone; when associated with T-310; I-371 or V-371. 0.1% activity and production of only flavone; when associated with T-310 and V-371. | ||||
Sequence: K → T |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000419822 | 1-523 | 2-hydroxyisoflavanone synthase | |||
Sequence: MLVELAITLLVIALFIHLRPTLSAKSKSLRHLPNPPSPKPRLPFVGHLHLLDKPLLHYSLIDLSKRYGPLYSLYFGSMPTVVASTPELFKLFLQTHEASSFNTRFQTSAIRRLTYDNSVAMVPFGPYWKFIRKLIMNDLLNATTVNKLRPLRSQEIRKVLRVMAQSAESQVPLNVTEELLKWTNSTISRMMLGEAEEIRDIARDVLKIFGEYSLTDFIWPLKKLKVGQYEKRIDDIFNRFDPVIERVIKKRQEIRKKRKERNGEIEEGEQSVVFLDTLLDFAEDETMEIKITKEQIKGLVVDFFSAGTDSTAVATDWALSELINNPRVFQKAREEIDAVVGKDRLVDEADVQNLPYIRSIVKETFRMHPPLPVVKRKCVQECEVDGYVIPEGALILFNVWAVGRDPKYWDRPTEFRPERFLENVGEGDQAVDLRGQHFQLLPFGSGRRMCPGVNLATAGMATLLASVIQCFDLSVVGPQGKILKGNDAKVSMEERAGLTVPRAHNLICVPVARSSAVPKLFSS |
Expression
Induction
Up-regulated upon elicitation.
Structure
Sequence
- Sequence statusComplete
- Length523
- Mass (Da)59,429
- Last updated2000-05-01 v1
- ChecksumA0E7F1127847BAB2
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB023636 EMBL· GenBank· DDBJ | BAA76380.1 EMBL· GenBank· DDBJ | mRNA |