Q9SXS3 · C93C2_GLYEC

Function

function

2-hydroxyisoflavanone synthase, which catalyzes the hydroxylation associated with 1,2-aryl migration of flavanones. Converts liquiritigenin and naringenin into highly unstable precursors of the isoflavones daidzein and genistein. Acts only on substrates with (2S)-chirality.

Miscellaneous

Lys-375 is essential for the intramolecular aryl migration of a flavanone molecule to produce the isoflavonoid skeleton (PubMed:12207646).
Arg-104 and Leu-371 are essential for the correct P450 conformation (PubMed:12207646, PubMed:15809082).

Catalytic activity

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
8 μMliquiritigenin

pH Dependence

Optimum pH is 7.5.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site450Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum
Cellular Componentintracellular membrane-bounded organelle
Cellular Componentmembrane
Molecular Function2-hydroxyisoflavanone synthase activity
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
Biological Processisoflavonoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    2-hydroxyisoflavanone synthase
  • EC number
  • Short names
    2HI synthase
  • Alternative names
    • CYP Ge-8
    • Cytochrome P450 93C2
    • Isoflavonoid synthase

Gene names

    • Name
      CYP93C2
    • Synonyms
      IFS

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > Hologalegina > IRL clade > Galegeae > Glycyrrhiza

Accessions

  • Primary accession
    Q9SXS3

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane2-22Helical

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis104Total loss of catalytic activity.
Mutagenesis106No effect on catalytic activity.
Mutagenesis119No effect on catalytic activity.
Mutagenesis305No effect on catalytic activity.
Mutagenesis310Decreased intramolecular 1,2-aryl migration and increased production of 3-hydroxyflavanone and flavone.
Mutagenesis310Decreased intramolecular 1,2-aryl migration and increased production of 3-hydroxyflavanone and flavone. 5% activity and production of mainly 3-hydroxyflavanone; when associated with T-375. 0.1% activity and production of only flavone; when associated with V-371 and T-375.
Mutagenesis371Production of mainly 3-hydroxyflavanone; when associated with T-375.
Mutagenesis371Total loss of catalytic activity. 5% activity and production of mainly 3-hydroxyflavanone; when associated with T-375. 0.1% activity and production of only flavone; when associated with T-310 and T-375.
Mutagenesis375Loss of intramolecular 1,2-aryl migration and production of only 3-hydroxyflavanone.
Mutagenesis375Loss of intramolecular 1,2-aryl migration and production of only 3-hydroxyflavanone. 5% activity and production of mainly 3-hydroxyflavanone; when associated with T-310; I-371 or V-371. 0.1% activity and production of only flavone; when associated with T-310 and V-371.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004198221-5232-hydroxyisoflavanone synthase

Expression

Induction

Up-regulated upon elicitation.

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    523
  • Mass (Da)
    59,429
  • Last updated
    2000-05-01 v1
  • Checksum
    A0E7F1127847BAB2
MLVELAITLLVIALFIHLRPTLSAKSKSLRHLPNPPSPKPRLPFVGHLHLLDKPLLHYSLIDLSKRYGPLYSLYFGSMPTVVASTPELFKLFLQTHEASSFNTRFQTSAIRRLTYDNSVAMVPFGPYWKFIRKLIMNDLLNATTVNKLRPLRSQEIRKVLRVMAQSAESQVPLNVTEELLKWTNSTISRMMLGEAEEIRDIARDVLKIFGEYSLTDFIWPLKKLKVGQYEKRIDDIFNRFDPVIERVIKKRQEIRKKRKERNGEIEEGEQSVVFLDTLLDFAEDETMEIKITKEQIKGLVVDFFSAGTDSTAVATDWALSELINNPRVFQKAREEIDAVVGKDRLVDEADVQNLPYIRSIVKETFRMHPPLPVVKRKCVQECEVDGYVIPEGALILFNVWAVGRDPKYWDRPTEFRPERFLENVGEGDQAVDLRGQHFQLLPFGSGRRMCPGVNLATAGMATLLASVIQCFDLSVVGPQGKILKGNDAKVSMEERAGLTVPRAHNLICVPVARSSAVPKLFSS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB023636
EMBL· GenBank· DDBJ
BAA76380.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp