Q9SWI1 · PKS1_ARATH
- ProteinProtein PHYTOCHROME KINASE SUBSTRATE 1
- GenePKS1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids439 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
May be responsible for light-regulated cytoplasmic sequestration of phytochromes or may be a negative regulator of phytochrome B signaling. Component of the network that modulates the very low-fluence response (VLFR) branch of phyA signaling. Acts positively in PHOT1 signaling. Regulates phytochrome-mediated photomorphogenesis and hypocotyl phototropism. Involved in the control of leaf flattening and leaf positioning. Promotes negative root phototropism and negatively regulates root gravitropism. May act by controlling auxin homeostasis.
Miscellaneous
PKS1, PKS2 and/or PKS4 are essential for phototropism but not for inhibition of gravitropism under long-term blue light irradiation.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | plasma membrane | |
Biological Process | phototropism | |
Biological Process | positive gravitropism | |
Biological Process | red or far-red light signaling pathway | |
Biological Process | red, far-red light phototransduction | |
Biological Process | response to far red light | |
Biological Process | response to red light |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameProtein PHYTOCHROME KINASE SUBSTRATE 1
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9SWI1
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Increased hypocotyl growth inhibition and cotyledon unfolding responses in the very low fluence response (VLFR) mode. Reduced phototropic response. Reduced hyponasty when grown under blue light. Loss of negative root phototropism. Auxin accumulation in protoplasts.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 57 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000058450 | 1-439 | Protein PHYTOCHROME KINASE SUBSTRATE 1 | |||
Sequence: MVTLTPSSASTPKTSFDFMKNNNSHSSLYVSSSSYLSSKEDALVTTKKLMEPSKTLNMSINPKQEEFGDEKKMVKKAPEDPEIGVFGAEKYFNGDMDSDQGSSVLSLTNPEVERTVVDSKQSAKKSTGTPSVRSESSWNSQSVLLQNKLVNSCNSSFKEKKNSNGQIQKVTNNKKSFLANLGCKCACSDGDSVDVEEKTSVKRSADPNISVITMRSSADMNTELIKIQKQEELSQRKSLEVFGSPVAIEKKSSVVQKKLPLPPWKSRTEEDDTKSEGSDSSSDLFEIEGLTGNPKPFLTRQGSDPASPTCYAPSEVSVEWSIVTASAADFSVMSECATSPVRRNRPTQIPRIPITAKSAPQRRKSSSSSGGNGFLMSCKSHKSVMVSGDLDRRSSMNKTQPSYVPRFPMETTKPKSFETRRRISNSSISHTQSSLLYSQ | ||||||
Modified residue | 238 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 244 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Phosphorylated on Ser and to a lower extent on Thr by phytochromes. Phosphorylation is stimulated twofold by red light.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in young seedlings in both darkness and light. Moderate in leaves and very low in roots and flowers. Expressed in the elongation zone of the root and hypocotyl.
Induction
Up-regulated by white, red, far-red and blue light.
Developmental stage
Decreases with development.
Gene expression databases
Interaction
Subunit
Interacts with PKS2, RPT3, PHOT1, PHOT2 and the C-termini of both phytochromes A (phyA) and B (phyB). Binds both spectral forms of phytochrome, Pr and Pfr.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9SWI1 | BPM3 O22286 | 3 | EBI-626200, EBI-540923 | |
BINARY | Q9SWI1 | BRX Q17TI5 | 3 | EBI-626200, EBI-4426649 | |
BINARY | Q9SWI1 | PHOT1 O48963 | 3 | EBI-626200, EBI-1553849 | |
BINARY | Q9SWI1 | PHYA P14712 | 3 | EBI-626200, EBI-624446 | |
BINARY | Q9SWI1 | PHYB P14713 | 2 | EBI-626200, EBI-300727 | |
BINARY | Q9SWI1 | RPT3 Q9FMF5 | 2 | EBI-626200, EBI-1553842 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-22 | Disordered | ||||
Sequence: MVTLTPSSASTPKTSFDFMKNN | ||||||
Region | 54-80 | Disordered | ||||
Sequence: KTLNMSINPKQEEFGDEKKMVKKAPED | ||||||
Compositional bias | 64-80 | Basic and acidic residues | ||||
Sequence: QEEFGDEKKMVKKAPED | ||||||
Region | 100-139 | Disordered | ||||
Sequence: QGSSVLSLTNPEVERTVVDSKQSAKKSTGTPSVRSESSWN | ||||||
Region | 259-311 | Disordered | ||||
Sequence: LPLPPWKSRTEEDDTKSEGSDSSSDLFEIEGLTGNPKPFLTRQGSDPASPTCY | ||||||
Compositional bias | 263-281 | Basic and acidic residues | ||||
Sequence: PWKSRTEEDDTKSEGSDSS | ||||||
Compositional bias | 296-310 | Polar residues | ||||
Sequence: PFLTRQGSDPASPTC | ||||||
Region | 355-439 | Disordered | ||||
Sequence: TAKSAPQRRKSSSSSGGNGFLMSCKSHKSVMVSGDLDRRSSMNKTQPSYVPRFPMETTKPKSFETRRRISNSSISHTQSSLLYSQ | ||||||
Compositional bias | 359-376 | Polar residues | ||||
Sequence: APQRRKSSSSSGGNGFLM | ||||||
Compositional bias | 390-406 | Polar residues | ||||
Sequence: LDRRSSMNKTQPSYVPR | ||||||
Compositional bias | 421-439 | Polar residues | ||||
Sequence: RRISNSSISHTQSSLLYSQ |
Sequence similarities
Belongs to the PKS family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length439
- Mass (Da)48,085
- Last updated2002-05-10 v2
- Checksum7A0A825FF7D3F3FC
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 64-80 | Basic and acidic residues | ||||
Sequence: QEEFGDEKKMVKKAPED | ||||||
Sequence conflict | 196 | in Ref. 1; AAD38033 | ||||
Sequence: E → D | ||||||
Compositional bias | 263-281 | Basic and acidic residues | ||||
Sequence: PWKSRTEEDDTKSEGSDSS | ||||||
Compositional bias | 296-310 | Polar residues | ||||
Sequence: PFLTRQGSDPASPTC | ||||||
Compositional bias | 359-376 | Polar residues | ||||
Sequence: APQRRKSSSSSGGNGFLM | ||||||
Compositional bias | 390-406 | Polar residues | ||||
Sequence: LDRRSSMNKTQPSYVPR | ||||||
Compositional bias | 421-439 | Polar residues | ||||
Sequence: RRISNSSISHTQSSLLYSQ |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF149053 EMBL· GenBank· DDBJ | AAD38033.1 EMBL· GenBank· DDBJ | mRNA | ||
AC004138 EMBL· GenBank· DDBJ | AAC32913.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC05643.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF325064 EMBL· GenBank· DDBJ | AAK17132.1 EMBL· GenBank· DDBJ | mRNA | ||
AY052708 EMBL· GenBank· DDBJ | AAK96612.1 EMBL· GenBank· DDBJ | mRNA | ||
AY063721 EMBL· GenBank· DDBJ | AAL36071.1 EMBL· GenBank· DDBJ | mRNA |