Q9SU14 · NUDT7_ARATH
- ProteinNudix hydrolase 7
- GeneNUDT7
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids282 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can use both NADH and ADP-ribose as substrates, but not 8-oxo-dGTP, cyclic ADP-ribose, GDP-mannose, UDP-glucose, ATP, or GTP. Exerts negative control of EDS1 signaling.
Catalytic activity
- ADP-D-ribose + H2O = D-ribose 5-phosphate + AMP + 2 H+
Cofactor
Activity regulation
Not inhibited by fluoride.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
26.23 μM | NADH | |||||
25.8 μM | ADP-ribose |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
3.681 nmol/min/mg | with NADH as substrate | ||||
2678 nmol/min/mg | with ADP-ribose as substrate |
pH Dependence
Optimum pH is 8.5.
Temperature Dependence
Optimum temperature is 50 degrees Celsius.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Cellular Component | plasma membrane | |
Molecular Function | ADP-ribose diphosphatase activity | |
Molecular Function | metal ion binding | |
Molecular Function | NAD binding | |
Molecular Function | NAD+ diphosphatase activity | |
Molecular Function | NADH pyrophosphatase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNudix hydrolase 7
- EC number
- Short namesAtNUDT7
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9SU14
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localized at the plasma membrane when in complex with GG2, but present in the cytoplasm when associated with GG1. Detected in the cytoplasm and nucleus when interacting with RACK1A.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Growth retardation, constitutive pathogen resistance phenotype and increased levels of reactive oxygen species and NADH.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 154 | Loss of hydrolase activity. | ||||
Sequence: E → Q |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 26 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000057127 | 1-282 | Nudix hydrolase 7 | |||
Sequence: MGTRAQQIPLLEGETDNYDGVTVTMVEPMDSEVFTESLRASLSHWREEGKKGIWIKLPLGLANLVEAAVSEGFRYHHAEPEYLMLVSWISETPDTIPANASHVVGAGALVINKNTKEVLVVQERSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETGIIADFVEVLAFRQSHKAILKKKTDMFFLCVLSPRSYDITEQKSEILQAKWMPIQEYVDQPWNKKNEMFKFMANICQKKCEEEYLGFAIVPTTTSSGKESFIYCNADHAKRLKVSRDQASASL |
Proteomic databases
Expression
Tissue specificity
Expressed in stems, leaves, roots, flowers and siliques.
Induction
Rapid and transient induction by biotic and abiotic stresses. Not induced by H2O2.
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 101-233 | Nudix hydrolase | ||||
Sequence: SHVVGAGALVINKNTKEVLVVQERSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETGIIADFVEVLAFRQSHKAILKKKTDMFFLCVLSPRSYDITEQKSEILQAKWMPIQEYVDQPWNKKNEMFKFMAN | ||||||
Motif | 139-160 | Nudix box | ||||
Sequence: GVINEGEDIWTGVAREVEEETG |
Sequence similarities
Belongs to the Nudix hydrolase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
Q9SU14-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length282
- Mass (Da)31,884
- Last updated2000-05-01 v1
- ChecksumEA37FC0F1C2FDCB7
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F4JRE7 | F4JRE7_ARATH | NUDT7 | 322 | ||
A0A1P8B842 | A0A1P8B842_ARATH | NUDT7 | 199 | ||
A0A1P8B846 | A0A1P8B846_ARATH | NUDT7 | 217 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 6 | in Ref. 4; AAM62604 | ||||
Sequence: Q → L | ||||||
Sequence conflict | 217 | in Ref. 4; AAM62604 | ||||
Sequence: V → I |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL049640 EMBL· GenBank· DDBJ | CAB40989.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL161534 EMBL· GenBank· DDBJ | CAB78314.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | AEE83167.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | AEE83168.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | AEE83169.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF325104 EMBL· GenBank· DDBJ | AAK17172.1 EMBL· GenBank· DDBJ | mRNA | ||
AF370209 EMBL· GenBank· DDBJ | AAK44024.1 EMBL· GenBank· DDBJ | mRNA | ||
AY056344 EMBL· GenBank· DDBJ | AAL07193.1 EMBL· GenBank· DDBJ | mRNA | ||
AY085375 EMBL· GenBank· DDBJ | AAM62604.1 EMBL· GenBank· DDBJ | mRNA |