Q9SU14 · NUDT7_ARATH

Function

function

Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can use both NADH and ADP-ribose as substrates, but not 8-oxo-dGTP, cyclic ADP-ribose, GDP-mannose, UDP-glucose, ATP, or GTP. Exerts negative control of EDS1 signaling.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Not inhibited by fluoride.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
26.23 μMNADH
25.8 μMADP-ribose
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
3.681 nmol/min/mgwith NADH as substrate
2678 nmol/min/mgwith ADP-ribose as substrate

pH Dependence

Optimum pH is 8.5.

Temperature Dependence

Optimum temperature is 50 degrees Celsius.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site154Mg2+ (UniProtKB | ChEBI)
Binding site158Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleus
Cellular Componentplasma membrane
Molecular FunctionADP-ribose diphosphatase activity
Molecular Functionmetal ion binding
Molecular FunctionNAD binding
Molecular FunctionNAD+ diphosphatase activity
Molecular FunctionNADH pyrophosphatase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nudix hydrolase 7
  • EC number
  • Short names
    AtNUDT7
  • Alternative names

Gene names

    • Name
      NUDT7
    • Synonyms
      GFG1, NUDX7
    • ORF names
      T20K18.70
    • Ordered locus names
      At4g12720

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9SU14
  • Secondary accessions
    • Q8LEK5

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Nucleus
Cytoplasm
Cell membrane
Note: Localized at the plasma membrane when in complex with GG2, but present in the cytoplasm when associated with GG1. Detected in the cytoplasm and nucleus when interacting with RACK1A.

Keywords

Phenotypes & Variants

Disruption phenotype

Growth retardation, constitutive pathogen resistance phenotype and increased levels of reactive oxygen species and NADH.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis154Loss of hydrolase activity.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 26 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000571271-282Nudix hydrolase 7

Proteomic databases

Expression

Tissue specificity

Expressed in stems, leaves, roots, flowers and siliques.

Induction

Rapid and transient induction by biotic and abiotic stresses. Not induced by H2O2.

Gene expression databases

Interaction

Subunit

Homodimer. Interacts with RACK1A, GG1 and GG2.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain101-233Nudix hydrolase
Motif139-160Nudix box

Sequence similarities

Belongs to the Nudix hydrolase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete

This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.

Q9SU14-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    282
  • Mass (Da)
    31,884
  • Last updated
    2000-05-01 v1
  • Checksum
    EA37FC0F1C2FDCB7
MGTRAQQIPLLEGETDNYDGVTVTMVEPMDSEVFTESLRASLSHWREEGKKGIWIKLPLGLANLVEAAVSEGFRYHHAEPEYLMLVSWISETPDTIPANASHVVGAGALVINKNTKEVLVVQERSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETGIIADFVEVLAFRQSHKAILKKKTDMFFLCVLSPRSYDITEQKSEILQAKWMPIQEYVDQPWNKKNEMFKFMANICQKKCEEEYLGFAIVPTTTSSGKESFIYCNADHAKRLKVSRDQASASL

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
F4JRE7F4JRE7_ARATHNUDT7322
A0A1P8B842A0A1P8B842_ARATHNUDT7199
A0A1P8B846A0A1P8B846_ARATHNUDT7217

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict6in Ref. 4; AAM62604
Sequence conflict217in Ref. 4; AAM62604

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL049640
EMBL· GenBank· DDBJ
CAB40989.1
EMBL· GenBank· DDBJ
Genomic DNA
AL161534
EMBL· GenBank· DDBJ
CAB78314.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002687
EMBL· GenBank· DDBJ
AEE83167.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002687
EMBL· GenBank· DDBJ
AEE83168.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002687
EMBL· GenBank· DDBJ
AEE83169.1
EMBL· GenBank· DDBJ
Genomic DNA
AF325104
EMBL· GenBank· DDBJ
AAK17172.1
EMBL· GenBank· DDBJ
mRNA
AF370209
EMBL· GenBank· DDBJ
AAK44024.1
EMBL· GenBank· DDBJ
mRNA
AY056344
EMBL· GenBank· DDBJ
AAL07193.1
EMBL· GenBank· DDBJ
mRNA
AY085375
EMBL· GenBank· DDBJ
AAM62604.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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