Q9SRK5 · LSF2_ARATH

Function

function

Starch-associated phosphoglucan phosphatase that selectively dephosphorylates the glucan C3 position. Probably participates in the regulation of starch degradation.

Features

Showing features for binding site, active site.

128220406080100120140160180200220240260280
TypeIDPosition(s)Description
Binding site83substrate
Binding site153-156substrate
Binding site161substrate
Binding site177-180substrate
Active site193Phosphocysteine intermediate
Binding site194-199substrate
Binding site230substrate
Binding site245substrate
Binding site251substrate
Binding site259-263substrate
Binding site268substrate

GO annotations

AspectTerm
Cellular Componentchloroplast
Molecular Functioncarbohydrate phosphatase activity
Molecular Functionphosphoprotein phosphatase activity
Molecular Functionstarch binding
Molecular Functionsugar-phosphatase activity
Biological Processstarch catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphoglucan phosphatase LSF2, chloroplastic
  • EC number
  • Alternative names
    • Phosphoglucan phosphatase like sex Four2
    • Protein LIKE SEX4 2

Gene names

    • Name
      LSF2
    • ORF names
      F9F8.24
    • Ordered locus names
      At3g10940

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9SRK5
  • Secondary accessions
    • Q8LBS6

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

No visible phenotype under normal growth conditions, but starch of mutant plants contains high levels of C3-bound phosphate.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis83Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate.
Mutagenesis85Nearly abolishes starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate.
Mutagenesis135Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate.
Mutagenesis136Abolishes starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate.
Mutagenesis153Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate.
Mutagenesis155Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate.
Mutagenesis157Decreases starch binding and starch C3 dephosphorylation. Moderate decrease of phosphatase activity with soluble substrates; when associated with A-261. Nearly abolishes activity with water-insoluble starch; when associated with A-261.
Mutagenesis162Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate.
Mutagenesis180Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate.
Mutagenesis193Abolishes phosphatase activity.
Mutagenesis194-198Abolishes glucan phosphatase activity.
Mutagenesis245Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate.
Mutagenesis261Strongly decreases starch binding and starch C3 dephosphorylation. Moderate decrease of phosphatase activity with soluble substrates; when associated with A-157. Nearly abolishes activity with water-insoluble starch; when associated with A-157.
Mutagenesis268Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 27 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-61Chloroplast
ChainPRO_000041733562-282Phosphoglucan phosphatase LSF2, chloroplastic

Proteomic databases

Expression

Tissue specificity

Widely expressed.

Induction

Expressed with a circadian rhythm showing a peak at the end of the day and then decreasing to reach the lowest levels at the end of the night.

Gene expression databases

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain92-249Tyrosine-protein phosphatase
Motif193-199Glucan phosphatase signature motif CXAGXGR

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    282
  • Mass (Da)
    32,087
  • Last updated
    2000-05-01 v1
  • Checksum
    BDB1A93855C98849
MSVIGSKSCIFSVARYTRENEKSSCFTSINKKSSLDLRFPRNLAGVSCKFSGENPGTNGVSLSSKNKMEDYNTAMKRLMRSPYEYHHDLGMNYTLIRDELIVGSQPQKPEDIDHLKQEQNVAYILNLQQDKDIEYWGIDLDSIVRRCKELGIRHMRRPAKDFDPLSLRSQLPKAVSSLEWAVSEGKGRVYVHCSAGLGRAPGVSIAYMYWFCDMNLNTAYDTLVSKRPCGPNKGAIRGATYDLAKNDPWKEPFESLPENAFEDIADWERKLIQERVRALRGT

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict38-39in Ref. 5; AAM64580

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC009991
EMBL· GenBank· DDBJ
AAF01527.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002686
EMBL· GenBank· DDBJ
AEE74980.1
EMBL· GenBank· DDBJ
Genomic DNA
BT024510
EMBL· GenBank· DDBJ
ABD19691.1
EMBL· GenBank· DDBJ
mRNA
AK226225
EMBL· GenBank· DDBJ
BAE98389.1
EMBL· GenBank· DDBJ
mRNA
AY087019
EMBL· GenBank· DDBJ
AAM64580.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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