Q9SRK5 · LSF2_ARATH
- ProteinPhosphoglucan phosphatase LSF2, chloroplastic
- GeneLSF2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids282 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Starch-associated phosphoglucan phosphatase that selectively dephosphorylates the glucan C3 position. Probably participates in the regulation of starch degradation.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 83 | substrate | ||||
Sequence: Y | ||||||
Binding site | 153-156 | substrate | ||||
Sequence: RHMR | ||||||
Binding site | 161 | substrate | ||||
Sequence: D | ||||||
Binding site | 177-180 | substrate | ||||
Sequence: SLEW | ||||||
Active site | 193 | Phosphocysteine intermediate | ||||
Sequence: C | ||||||
Binding site | 194-199 | substrate | ||||
Sequence: SAGLGR | ||||||
Binding site | 230 | substrate | ||||
Sequence: G | ||||||
Binding site | 245 | substrate | ||||
Sequence: K | ||||||
Binding site | 251 | substrate | ||||
Sequence: E | ||||||
Binding site | 259-263 | substrate | ||||
Sequence: NAFED | ||||||
Binding site | 268 | substrate | ||||
Sequence: E |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | carbohydrate phosphatase activity | |
Molecular Function | phosphoprotein phosphatase activity | |
Molecular Function | starch binding | |
Molecular Function | sugar-phosphatase activity | |
Biological Process | starch catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoglucan phosphatase LSF2, chloroplastic
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9SRK5
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
No visible phenotype under normal growth conditions, but starch of mutant plants contains high levels of C3-bound phosphate.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 83 | Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 85 | Nearly abolishes starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 135 | Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 136 | Abolishes starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate. | ||||
Sequence: W → A | ||||||
Mutagenesis | 153 | Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate. | ||||
Sequence: R → A | ||||||
Mutagenesis | 155 | Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate. | ||||
Sequence: M → A | ||||||
Mutagenesis | 157 | Decreases starch binding and starch C3 dephosphorylation. Moderate decrease of phosphatase activity with soluble substrates; when associated with A-261. Nearly abolishes activity with water-insoluble starch; when associated with A-261. | ||||
Sequence: R → A | ||||||
Mutagenesis | 162 | Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate. | ||||
Sequence: F → A | ||||||
Mutagenesis | 180 | Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate. | ||||
Sequence: W → A | ||||||
Mutagenesis | 193 | Abolishes phosphatase activity. | ||||
Sequence: C → S | ||||||
Mutagenesis | 194-198 | Abolishes glucan phosphatase activity. | ||||
Sequence: SAGLG → TTGFD | ||||||
Mutagenesis | 245 | Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate. | ||||
Sequence: K → A | ||||||
Mutagenesis | 261 | Strongly decreases starch binding and starch C3 dephosphorylation. Moderate decrease of phosphatase activity with soluble substrates; when associated with A-157. Nearly abolishes activity with water-insoluble starch; when associated with A-157. | ||||
Sequence: F → A | ||||||
Mutagenesis | 268 | Decreases starch C3 dephosphorylation. No effect on phosphatase activity with p-nitrophenyl phosphate. | ||||
Sequence: E → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 27 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-61 | Chloroplast | ||||
Sequence: MSVIGSKSCIFSVARYTRENEKSSCFTSINKKSSLDLRFPRNLAGVSCKFSGENPGTNGVS | ||||||
Chain | PRO_0000417335 | 62-282 | Phosphoglucan phosphatase LSF2, chloroplastic | |||
Sequence: LSSKNKMEDYNTAMKRLMRSPYEYHHDLGMNYTLIRDELIVGSQPQKPEDIDHLKQEQNVAYILNLQQDKDIEYWGIDLDSIVRRCKELGIRHMRRPAKDFDPLSLRSQLPKAVSSLEWAVSEGKGRVYVHCSAGLGRAPGVSIAYMYWFCDMNLNTAYDTLVSKRPCGPNKGAIRGATYDLAKNDPWKEPFESLPENAFEDIADWERKLIQERVRALRGT |
Proteomic databases
Expression
Tissue specificity
Widely expressed.
Induction
Expressed with a circadian rhythm showing a peak at the end of the day and then decreasing to reach the lowest levels at the end of the night.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 92-249 | Tyrosine-protein phosphatase | ||||
Sequence: NYTLIRDELIVGSQPQKPEDIDHLKQEQNVAYILNLQQDKDIEYWGIDLDSIVRRCKELGIRHMRRPAKDFDPLSLRSQLPKAVSSLEWAVSEGKGRVYVHCSAGLGRAPGVSIAYMYWFCDMNLNTAYDTLVSKRPCGPNKGAIRGATYDLAKNDPW | ||||||
Motif | 193-199 | Glucan phosphatase signature motif CXAGXGR | ||||
Sequence: CSAGLGR |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length282
- Mass (Da)32,087
- Last updated2000-05-01 v1
- ChecksumBDB1A93855C98849
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 38-39 | in Ref. 5; AAM64580 | ||||
Sequence: RF → SL |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC009991 EMBL· GenBank· DDBJ | AAF01527.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE74980.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT024510 EMBL· GenBank· DDBJ | ABD19691.1 EMBL· GenBank· DDBJ | mRNA | ||
AK226225 EMBL· GenBank· DDBJ | BAE98389.1 EMBL· GenBank· DDBJ | mRNA | ||
AY087019 EMBL· GenBank· DDBJ | AAM64580.1 EMBL· GenBank· DDBJ | mRNA |