Q9SQR2 · ADRC2_ARATH
- ProteinNADPH-dependent aldehyde reductase 2, chloroplastic
- GeneChlADR2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids272 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Aldehyde reductase that catalyzes the reduction of the aldehyde carbonyl groups on saturated and alpha,beta-unsaturated aldehydes with more than 5 carbons (PubMed:21169366).
No activity on alpha,beta-unsaturated ketones (PubMed:21169366).
Can use propionaldehyde, butyraldehyde, methylglyoxal, (e)-2-pentenal, (E)-2-hexenal, (Z)-3-hexenal and (E)-2-nonenal as substrates, but not propenal (acrolein), crotonaldehyde, 2-butanone, 3-buten-2-one or 1-penten-3-one (PubMed:21169366).
No activity on alpha,beta-unsaturated ketones (PubMed:21169366).
Can use propionaldehyde, butyraldehyde, methylglyoxal, (e)-2-pentenal, (E)-2-hexenal, (Z)-3-hexenal and (E)-2-nonenal as substrates, but not propenal (acrolein), crotonaldehyde, 2-butanone, 3-buten-2-one or 1-penten-3-one (PubMed:21169366).
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
7.8 mM | butyraldehyde | |||||
0.6 mM | (E)-2-pentenal | |||||
2.5 mM | (E)-2-hexenal | |||||
5 mM | methylglyoxal |
kcat is 29.6 sec-1 for butyraldehyde. kcat is 6.4 sec-1 for (E)-2-pentenal. kcat is 1.8 sec-1 for (E)-2-hexenal. kcat is 28.8 sec-1 for methylglyoxal.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | alcohol dehydrogenase (NADP+) activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADPH-dependent aldehyde reductase 2, chloroplastic
- EC number
- Short namesAtChlADR2
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9SQR2
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-53 | Chloroplast | ||||
Sequence: MAAASSVSSPPLCLAGRVAIVTGSSRGIGRAIAIHLAELGARVVVNYSTSPVE | ||||||
Chain | PRO_0000439504 | 54-272 | NADPH-dependent aldehyde reductase 2, chloroplastic | |||
Sequence: AEKVATAITTNCSKDAEVAGKSPRVIVVKADISEPSQVKSLFDEAERVFESPVHILVNSAAIADPNHSTISDMSVELFDRIISVNTRGAFICAREAANRLKRGGGGRIILLSTSLVQTLNTNYGSYTASKAAVEAMAKILAKELKGTEITVNCVSPGPVATEMFYTGLSNEIVEKVKSQNLFGRIGETKDIAPVVGFLASDAGEWINGQVIMANGGCLL |
Proteomic databases
Expression
Gene expression databases
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9SQR2 | At4g13180 Q9SVQ9 | 3 | EBI-4471202, EBI-4471207 |
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length272
- Mass (Da)28,435
- Last updated2000-05-01 v1
- Checksum1F0542EE7C599276
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC011698 EMBL· GenBank· DDBJ | AAF05859.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE74024.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT002321 EMBL· GenBank· DDBJ | AAN86154.1 EMBL· GenBank· DDBJ | mRNA | ||
AY045807 EMBL· GenBank· DDBJ | AAK76481.2 EMBL· GenBank· DDBJ | mRNA |