Q9SQ80 · G2OX1_PEA
- ProteinGibberellin 2-beta-dioxygenase 1
- GeneGA2OX1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids327 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the 2-beta-hydroxylation of several biologically active gibberellins, leading to the homeostatic regulation of their endogenous level. Catabolism of gibberellins (GAs) plays a central role in plant development. Converts GA9/GA20 to GA51/GA29 and GA4/GA1 to GA34/GA8.
Catalytic activity
- gibberellin A1 + 2-oxoglutarate + O2 = gibberellin A8 + succinate + CO2
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.024 μM | GA20 |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
4.4 pmol/sec/mg |
Pathway
Plant hormone biosynthesis; gibberellin biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 200 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 202 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 257 | Fe cation (UniProtKB | ChEBI) | |||
Active site | 267 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | C-19 gibberellin 2-beta-dioxygenase activity | |
Molecular Function | metal ion binding | |
Biological Process | gibberellin biosynthetic process | |
Biological Process | gibberellin catabolic process | |
Biological Process | response to light stimulus |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGibberellin 2-beta-dioxygenase 1
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > Hologalegina > IRL clade > Fabeae > Pisum
Accessions
- Primary accessionQ9SQ80
- Secondary accessions
Phenotypes & Variants
Disruption phenotype
Plants display to the slender phenotype, characterized by large amounts of GA20 that cannot be degraded, and is metabolized to excess GA1 on germination, producing plants with a characteristic slender or hyperelongated phenotype.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000067311 | 1-327 | Gibberellin 2-beta-dioxygenase 1 | ||
Expression
Tissue specificity
Predominantly expressed in roots, flowers, young fruits and seeds.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 171-276 | Fe2OG dioxygenase | |||
Sequence similarities
Belongs to the iron/ascorbate-dependent oxidoreductase family. GA2OX subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length327
- Mass (Da)36,811
- Last updated2000-05-01 v1
- Checksum2728A3B5C871CFB2
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 116 | in Ref. 1; AAF13734 and 2; AAD45425 | |||
Sequence conflict | 127 | in Ref. 1; AAF08609 | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF056935 EMBL· GenBank· DDBJ | AAF08609.1 EMBL· GenBank· DDBJ | mRNA | ||
AF101382 EMBL· GenBank· DDBJ | AAF13734.1 EMBL· GenBank· DDBJ | mRNA | Sequence problems. | |
AF101383 EMBL· GenBank· DDBJ | AAF13735.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF100955 EMBL· GenBank· DDBJ | AAD45425.1 EMBL· GenBank· DDBJ | mRNA |