Q9SMJ3 · ZDS_CAPAN
- ProteinZeta-carotene desaturase, chloroplastic/chromoplastic
- GeneZDS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids588 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the conversion of zeta-carotene to lycopene via the intermediary of neurosporene. It carries out two consecutive desaturations (introduction of double bonds) at positions C-7 and C-7'. Shows stereoselectivity toward trans C15-C15'zeta-carotene double bond. The zeta-carotene produced by the phytoene desaturase PDS has a C15-C15' double bond in the cis configuration and it requires isomerization before being recognized as substrate by ZDS. No activity with all-trans-zeta-carotene. The main product is 7,9,7',9'-tetra-cis-lycopene (pro-lycopene).
Catalytic activity
- 9,9'-di-cis-zeta-carotene + 2 a quinone = 7,7',9,9'-tetra-cis-lycopene + 2 a quinol
Cofactor
6-decylubiquinone (UniProtKB | Rhea| CHEBI:52020 )
Note: Lipophilic quinones such as decyl-plastoquinone or decyl-ubiquinone.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
8.4 μM | zeta-carotene | |||||
9 μM | neurosporene |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
0.665 nmol/h/mg | with zeta-carotene as substrate | ||||
0.0542 nmol/h/mg | with neurosporene as substrate |
Pathway
Carotenoid biosynthesis; lycopene biosynthesis.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | chromoplast | |
Molecular Function | 7,9,9'-tricis-neurosporene:quinone oxidoreductase activity | |
Molecular Function | 9,9'-di-cis-zeta-carotene desaturase activity | |
Molecular Function | 9,9'-dicis-carotene:quinone oxidoreductase activity | |
Biological Process | carotene biosynthetic process | |
Biological Process | carotenoid biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameZeta-carotene desaturase, chloroplastic/chromoplastic
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Solanoideae > Capsiceae > Capsicum
Accessions
- Primary accessionQ9SMJ3
Subcellular Location
Phenotypes & Variants
Chemistry
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-49 | Chloroplast and chromoplast | ||||
Sequence: MATCSAYLCCPATSASLKKRVFPDGSAGFLFFGGRRLSNRLVTPKSVIR | ||||||
Chain | PRO_0000041606 | 50-588 | Zeta-carotene desaturase, chloroplastic/chromoplastic | |||
Sequence: ADLNSMVSDMSTNAPKGLFPPEPEHYRGPKLKVAIIGAGLAGMSTAVELLDQGHEVDIYESRTFIGGKVGSFVDKRGNHIEMGLHVFFGCYNNLFRLMKKVGAEKNLLVKEHTHTFVNKGGEIGELDFRFPVGAPLHGINAFLSTNQLKTYDKARNAVALALSPVVRALVDPDGALQQIRDLDSVSFSDWFMSKGGTRASIQRMWDPVAYALGFIDCDNISARCMLTIFALFATKTEASLLRMLKGSPDVYLSGPIKKYIIDKGGRFHLRWGCREVLYETSSDGSMYVSGLAMSKATQKKIVKADAYVAACVVPGIKRLVPQKWRELEFFGNIYKLIGVPVVTVQLRYNGWVTELQDLERSRQSKRATGLDNLLYTPDADFSCFADLALASPEDYYIEGQGSLLQCVLTPGDPYMPLPNEEIIRRVSKQVLALFPSSQGLEVTWSSVVKIGQSLYREGPGKDPFRPDQKTPVENFFLAGSYTKQDYIDSMEGATLSGRQASAYICDAGEQLLALRKKIAAAELNEISKGVSLSDELSLV |
Interaction
Subunit
Monomer and dimer.
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length588
- Mass (Da)64,684
- Last updated2000-05-01 v1
- Checksum55F5668FAEE7EA91
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X89897 EMBL· GenBank· DDBJ | CAA61985.1 EMBL· GenBank· DDBJ | mRNA |