Q9SJZ6 · MED18_ARATH

Function

function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors (PubMed:22021418, PubMed:24451981).
Involved in the regulation of histone H3 lysine tri-methylation (H3K36me3). Associates with the promoter, coding and terminator regions of target genes suggesting its function in transcription initiation, elongation and termination. Multifunctional protein which regulates plant immunity, especially during necrotrophic fungal infection (e.g. B.cinerea and A.brassicicola), flowering time and responses to hormones (e.g. abscisic acid ABA and ethylene) through interactions with distinct transcription factors (PubMed:24451981).

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentmediator complex
Cellular Componentnucleus
Molecular Functiontranscription coregulator activity
Biological Processabscisic acid-activated signaling pathway
Biological Processcarpel development
Biological Processdefense response
Biological ProcessDNA-templated transcription termination
Biological Processethylene-activated signaling pathway
Biological ProcessmiRNA processing
Biological Processnegative regulation of DNA-templated transcription
Biological Processpetal development
Biological Processpositive regulation of transcription initiation by RNA polymerase II
Biological Processregulation of defense response to fungus
Biological Processregulation of DNA-templated transcription
Biological Processregulation of DNA-templated transcription elongation
Biological Processregulation of DNA-templated transcription initiation
Biological Processregulation of photoperiodism, flowering
Biological Processregulation of salicylic acid mediated signaling pathway
Biological Processregulation of termination of DNA-templated transcription
Biological Processregulation of timing of transition from vegetative to reproductive phase
Biological Processregulation of vernalization response
Biological Processresponse to abscisic acid
Biological Processresponse to ethylene
Biological Processsepal development
Biological Processspecification of floral organ number
Biological Processstamen development

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Mediator of RNA polymerase II transcription subunit 18

Gene names

    • Name
      MED18
    • Synonyms
      MED18_1
    • ORF names
      F14M13.23
    • Ordered locus names
      At2g22370

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9SJZ6

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Deregulated expression of glutaredoxins GRX480, GRXS13 and thioredoxin TRX-h5 leading to enhanced susceptibility to fungal infection (e.g. B.cinerea and A.brassicicola). Altered RNA polymerase II occupancy and histone H3 lysine tri-methylation (H3K36me3) of target genes. Insensitivity to abscisic acid (ABA) and to the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC). Delayed flowering.

Variants

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The viewer provides 3 variants from UniProt as well as other sources including ClinVar and dbSNP.

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PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004183521-219Mediator of RNA polymerase II transcription subunit 18

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

Component of the Mediator complex (PubMed:17560376).
Interacts with YY1 to suppress disease susceptibility via the repression of genes glutaredoxins GRX480, GRXS13 and thioredoxin TRX-h5. Binds to ABI4 to regulate abscisic acid responses; recruited by ABI4 to ABI5 promoter in the presence of abscisic acid (ABA). Interacts with SUF4 to regulate flowering time; recruited by SUF4 to FLC promoter (PubMed:24451981).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntAct
BINARY Q9SJZ6MED20A Q6NPF43EBI-1386265, EBI-1386270

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the Mediator complex subunit 18 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    219
  • Mass (Da)
    23,365
  • Last updated
    2000-05-01 v1
  • Checksum
    FE53D1C081F36F55
MSMECVVQGIIETQHVEALEILLQGLCGVQRERLRVHELCLRSGPNLGVVSSEVRLLCDLDQPEPTWTVKHVGGAMRGAGADQISVLVRNMIESKVSKNALRMFYALGYKLDHELLKVGFAFHFQRTAHISVSVSSVNKMPKVHAIDEAVPVTPGMQIVDVTAPATSENYSEVAAAVSSFCEFLAPLVHLSKPSISTGVVPTAAAAAASLMSDGGGTTL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC006592
EMBL· GenBank· DDBJ
AAD22361.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002685
EMBL· GenBank· DDBJ
AEC07298.1
EMBL· GenBank· DDBJ
Genomic DNA
BT029378
EMBL· GenBank· DDBJ
ABK32192.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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