Q9SJZ6 · MED18_ARATH
- ProteinMediator of RNA polymerase II transcription subunit 18
- GeneMED18
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids219 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors (PubMed:22021418, PubMed:24451981).
Involved in the regulation of histone H3 lysine tri-methylation (H3K36me3). Associates with the promoter, coding and terminator regions of target genes suggesting its function in transcription initiation, elongation and termination. Multifunctional protein which regulates plant immunity, especially during necrotrophic fungal infection (e.g. B.cinerea and A.brassicicola), flowering time and responses to hormones (e.g. abscisic acid ABA and ethylene) through interactions with distinct transcription factors (PubMed:24451981).
Involved in the regulation of histone H3 lysine tri-methylation (H3K36me3). Associates with the promoter, coding and terminator regions of target genes suggesting its function in transcription initiation, elongation and termination. Multifunctional protein which regulates plant immunity, especially during necrotrophic fungal infection (e.g. B.cinerea and A.brassicicola), flowering time and responses to hormones (e.g. abscisic acid ABA and ethylene) through interactions with distinct transcription factors (PubMed:24451981).
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameMediator of RNA polymerase II transcription subunit 18
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9SJZ6
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Deregulated expression of glutaredoxins GRX480, GRXS13 and thioredoxin TRX-h5 leading to enhanced susceptibility to fungal infection (e.g. B.cinerea and A.brassicicola). Altered RNA polymerase II occupancy and histone H3 lysine tri-methylation (H3K36me3) of target genes. Insensitivity to abscisic acid (ABA) and to the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC). Delayed flowering.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 3 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000418352 | 1-219 | Mediator of RNA polymerase II transcription subunit 18 | ||
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
Component of the Mediator complex (PubMed:17560376).
Interacts with YY1 to suppress disease susceptibility via the repression of genes glutaredoxins GRX480, GRXS13 and thioredoxin TRX-h5. Binds to ABI4 to regulate abscisic acid responses; recruited by ABI4 to ABI5 promoter in the presence of abscisic acid (ABA). Interacts with SUF4 to regulate flowering time; recruited by SUF4 to FLC promoter (PubMed:24451981).
Interacts with YY1 to suppress disease susceptibility via the repression of genes glutaredoxins GRX480, GRXS13 and thioredoxin TRX-h5. Binds to ABI4 to regulate abscisic acid responses; recruited by ABI4 to ABI5 promoter in the presence of abscisic acid (ABA). Interacts with SUF4 to regulate flowering time; recruited by SUF4 to FLC promoter (PubMed:24451981).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | Q9SJZ6 | MED20A Q6NPF4 | 3 | EBI-1386265, EBI-1386270 |
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length219
- Mass (Da)23,365
- Last updated2000-05-01 v1
- ChecksumFE53D1C081F36F55
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC006592 EMBL· GenBank· DDBJ | AAD22361.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC07298.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT029378 EMBL· GenBank· DDBJ | ABK32192.1 EMBL· GenBank· DDBJ | mRNA |