Q9SJY5 · PUMP5_ARATH
- ProteinMitochondrial uncoupling protein 5
- GenePUMP5
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids313 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
PUMPS are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. This leads to a decrease in the efficiency of oxidative phosphorylation and an increase in heat production. May be involved in protecting plant cells against oxidative stress damage. Recombinant PUMP5, reconstituted into liposomes, transports a wide range of dicarboxylic acids including malate, oxaloacetate and succinate as well as phosphate, sulfate and thiosulfate. However, it is unknown if these transports are of any biological significance in vivo.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial inner membrane | |
Molecular Function | dicarboxylic acid transmembrane transporter activity | |
Molecular Function | oxidative phosphorylation uncoupler activity | |
Biological Process | cellular response to hypoxia | |
Biological Process | mitochondrial transport | |
Biological Process | proton transmembrane transport |
Keywords
- Biological process
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameMitochondrial uncoupling protein 5
- Short namesAtPUMP5
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9SJY5
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Mitochondrion inner membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 6-26 | Helical; Name=1 | ||||
Sequence: FAEGGIASIVAGCSTHPLDLI | ||||||
Transmembrane | 77-97 | Helical; Name=2 | ||||
Sequence: MRALFSGVSATVLRQTLYSTT | ||||||
Transmembrane | 123-143 | Helical; Name=3 | ||||
Sequence: IGAGAIAGAIGAAVGNPADVA | ||||||
Transmembrane | 182-202 | Helical; Name=4 | ||||
Sequence: RGSSLTINRAMLVTSSQLASY | ||||||
Transmembrane | 223-243 | Helical; Name=5 | ||||
Sequence: VSASFAAGFVASVASNPVDVI | ||||||
Transmembrane | 280-300 | Helical; Name=6 | ||||
Sequence: YKGFIPTVSRQAPFTVVLFVT |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000420259 | 1-313 | Mitochondrial uncoupling protein 5 | |||
Sequence: MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLQGESAPIQTNLRPALAFQTSTTVNAPPLRVGVIGVGSRLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKGEWTDPETKTMPLMKKIGAGAIAGAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDAITQMIRGEGVTSLWRGSSLTINRAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASFAAGFVASVASNPVDVIKTRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTVSRQAPFTVVLFVTLEQVKKLFKDYDF |
Proteomic databases
Expression
Tissue specificity
Expressed in roots, leaves, stems and flowers.
Induction
By cold stress and high light.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Repeat | 4-108 | Solcar 1 | ||||
Sequence: KGFAEGGIASIVAGCSTHPLDLIKVRMQLQGESAPIQTNLRPALAFQTSTTVNAPPLRVGVIGVGSRLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKGE | ||||||
Repeat | 117-208 | Solcar 2 | ||||
Sequence: MPLMKKIGAGAIAGAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDAITQMIRGEGVTSLWRGSSLTINRAMLVTSSQLASYDSVKET | ||||||
Repeat | 217-307 | Solcar 3 | ||||
Sequence: DGLGTHVSASFAAGFVASVASNPVDVIKTRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTVSRQAPFTVVLFVTLEQVKKL |
Sequence similarities
Belongs to the mitochondrial carrier (TC 2.A.29) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length313
- Mass (Da)33,357
- Last updated2000-05-01 v1
- Checksum447903EAA3865E6C
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 130 | in Ref. 4; AAK44155 | ||||
Sequence: G → V |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AM236862 EMBL· GenBank· DDBJ | CAJ86454.1 EMBL· GenBank· DDBJ | mRNA | ||
AC006592 EMBL· GenBank· DDBJ | AAD22351.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC07315.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF370340 EMBL· GenBank· DDBJ | AAK44155.1 EMBL· GenBank· DDBJ | mRNA | ||
AF370588 EMBL· GenBank· DDBJ | AAK43907.1 EMBL· GenBank· DDBJ | mRNA | ||
AY142648 EMBL· GenBank· DDBJ | AAN13106.1 EMBL· GenBank· DDBJ | mRNA | ||
AK230325 EMBL· GenBank· DDBJ | BAF02125.1 EMBL· GenBank· DDBJ | mRNA | Frameshift |