Q9SJN0 · ABI5_ARATH

Function

function

Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Can also trans-activate its own promoter, suggesting that it is autoregulated. Plays a role in sugar-mediated senescence.

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentnucleus
Molecular FunctionDNA-binding transcription factor activity
Molecular Functiontranscription cis-regulatory region binding
Biological Processabscisic acid-activated signaling pathway
Biological Processnegative regulation of seed germination
Biological Processpositive regulation of DNA-templated transcription
Biological Processregulation of DNA-templated transcription
Biological Processresponse to abscisic acid
Biological Processresponse to gibberellin
Biological Processresponse to salt stress
Biological Processresponse to water deprivation
Biological Processseed development
Biological Processseed germination
Biological Processsugar mediated signaling pathway

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Protein ABSCISIC ACID-INSENSITIVE 5
  • Alternative names
    • Dc3 promoter-binding factor 1 (AtDPBF1)
    • Protein GROWTH-INSENSITIVITY TO ABA 1
    • bZIP transcription factor 39 (AtbZIP39)

Gene names

    • Name
      ABI5
    • Synonyms
      BZIP39, DPBF1, GIA1, NEM1
    • ORF names
      F2H17.12
    • Ordered locus names
      At2g36270

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9SJN0

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Nucleus

Keywords

Phenotypes & Variants

Disruption phenotype

Exhibits abscisic acid (ABA) insensitivity.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis391Loss of sumoylation.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 27 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue, cross-link.

TypeIDPosition(s)Description
ChainPRO_00003696051-442Protein ABSCISIC ACID-INSENSITIVE 5
Modified residue42Phosphoserine
Modified residue64Phosphoserine
Modified residue145Phosphoserine
Modified residue201Phosphothreonine
Cross-link391Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Post-translational modification

Phosphorylated by SRK2D and SRK2I in vitro.
Ubiquitinated. AFP1, KEG and RPN10 mediate its proteasome-dependent degradation. Its stability or degradation plays a central role in abscisic acid response. Sumoylated at Lys-391 by SIZ1. Sumoylation protects ABI5 from proteasome degradation, attenuating ABA signaling and sensitivity to ABA.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Predominantly expressed in seeds.

Induction

Up-regulated by drought, salt, abscisic acid (ABA) and glucose or 2-deoxy-glucose (2DG). Autoregulated. Positively regulated by the light-signaling component HY5.

Developmental stage

Expressed in embryo during the latest stages of seed maturation.

Gene expression databases

Interaction

Subunit

DNA-binding homodimer. DNA-binding heterodimer with AREB3/DPBF3 or EEL/DPBF4. Interacts with ABI3, KEG, the mediator subunit MED25, and the AFP proteins AFP1, AFP2, AFP3 and AFP4. Interacts with TAP46. Interacts with the 36 kDa catalytic subunit (subunit C) of PP2A (PubMed:11489176, PubMed:12569131, PubMed:16247556, PubMed:17194765, PubMed:18484180, PubMed:22822206, PubMed:24357600).
Interacts with FYPP1 and FYPP3 (PubMed:22715043).
Interacts with FREE1 (via C-terminus) (PubMed:30962512).

Binary interactions

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for compositional bias, region, domain.

TypeIDPosition(s)Description
Compositional bias1-18Basic and acidic residues
Region1-37Disordered
Compositional bias83-113Polar residues
Region83-151Disordered
Compositional bias123-145Polar residues
Region164-201Disordered
Compositional bias173-197Polar residues
Region218-238Disordered
Domain355-418bZIP
Region357-376Basic motif
Region383-404Leucine-zipper
Region414-442Disordered

Sequence similarities

Belongs to the bZIP family. ABI5 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    442
  • Mass (Da)
    47,007
  • Last updated
    2000-05-01 v1
  • Checksum
    F879F51B99A99140
MVTRETKLTSEREVESSMAQARHNGGGGGENHPFTSLGRQSSIYSLTLDEFQHALCENGKNFGSMNMDEFLVSIWNAEENNNNQQQAAAAAGSHSVPANHNGFNNNNNNGGEGGVGVFSGGSRGNEDANNKRGIANESSLPRQGSLTLPAPLCRKTVDEVWSEIHRGGGSGNGGDSNGRSSSSNGQNNAQNGGETAARQPTFGEMTLEDFLVKAGVVREHPTNPKPNPNPNQNQNPSSVIPAAAQQQLYGVFQGTGDPSFPGQAMGVGDPSGYAKRTGGGGYQQAPPVQAGVCYGGGVGFGAGGQQMGMVGPLSPVSSDGLGHGQVDNIGGQYGVDMGGLRGRKRVVDGPVEKVVERRQRRMIKNRESAARSRARKQAYTVELEAELNQLKEENAQLKHALAELERKRKQQYFESLKSRAQPKLPKSNGRLRTLMRNPSCPL

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1P8B106A0A1P8B106_ARATHABI5444

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-18Basic and acidic residues
Compositional bias83-113Polar residues
Compositional bias123-145Polar residues
Compositional bias173-197Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF334206
EMBL· GenBank· DDBJ
AAK19599.1
EMBL· GenBank· DDBJ
mRNA
AC006921
EMBL· GenBank· DDBJ
AAD21438.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002685
EMBL· GenBank· DDBJ
AEC09226.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002685
EMBL· GenBank· DDBJ
ANM62587.1
EMBL· GenBank· DDBJ
Genomic DNA
BT026517
EMBL· GenBank· DDBJ
ABH04624.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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