Q9SJN0 · ABI5_ARATH
- ProteinProtein ABSCISIC ACID-INSENSITIVE 5
- GeneABI5
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids442 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Can also trans-activate its own promoter, suggesting that it is autoregulated. Plays a role in sugar-mediated senescence.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | DNA-binding transcription factor activity | |
Molecular Function | transcription cis-regulatory region binding | |
Biological Process | abscisic acid-activated signaling pathway | |
Biological Process | negative regulation of seed germination | |
Biological Process | positive regulation of DNA-templated transcription | |
Biological Process | regulation of DNA-templated transcription | |
Biological Process | response to abscisic acid | |
Biological Process | response to gibberellin | |
Biological Process | response to salt stress | |
Biological Process | response to water deprivation | |
Biological Process | seed development | |
Biological Process | seed germination | |
Biological Process | sugar mediated signaling pathway |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameProtein ABSCISIC ACID-INSENSITIVE 5
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9SJN0
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Exhibits abscisic acid (ABA) insensitivity.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 391 | Loss of sumoylation. | ||||
Sequence: K → R |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 27 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000369605 | 1-442 | Protein ABSCISIC ACID-INSENSITIVE 5 | |||
Sequence: MVTRETKLTSEREVESSMAQARHNGGGGGENHPFTSLGRQSSIYSLTLDEFQHALCENGKNFGSMNMDEFLVSIWNAEENNNNQQQAAAAAGSHSVPANHNGFNNNNNNGGEGGVGVFSGGSRGNEDANNKRGIANESSLPRQGSLTLPAPLCRKTVDEVWSEIHRGGGSGNGGDSNGRSSSSNGQNNAQNGGETAARQPTFGEMTLEDFLVKAGVVREHPTNPKPNPNPNQNQNPSSVIPAAAQQQLYGVFQGTGDPSFPGQAMGVGDPSGYAKRTGGGGYQQAPPVQAGVCYGGGVGFGAGGQQMGMVGPLSPVSSDGLGHGQVDNIGGQYGVDMGGLRGRKRVVDGPVEKVVERRQRRMIKNRESAARSRARKQAYTVELEAELNQLKEENAQLKHALAELERKRKQQYFESLKSRAQPKLPKSNGRLRTLMRNPSCPL | ||||||
Modified residue | 42 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 64 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 145 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 201 | Phosphothreonine | ||||
Sequence: T | ||||||
Cross-link | 391 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) | ||||
Sequence: K |
Post-translational modification
Phosphorylated by SRK2D and SRK2I in vitro.
Ubiquitinated. AFP1, KEG and RPN10 mediate its proteasome-dependent degradation. Its stability or degradation plays a central role in abscisic acid response. Sumoylated at Lys-391 by SIZ1. Sumoylation protects ABI5 from proteasome degradation, attenuating ABA signaling and sensitivity to ABA.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Predominantly expressed in seeds.
Induction
Up-regulated by drought, salt, abscisic acid (ABA) and glucose or 2-deoxy-glucose (2DG). Autoregulated. Positively regulated by the light-signaling component HY5.
Developmental stage
Expressed in embryo during the latest stages of seed maturation.
Gene expression databases
Interaction
Subunit
DNA-binding homodimer. DNA-binding heterodimer with AREB3/DPBF3 or EEL/DPBF4. Interacts with ABI3, KEG, the mediator subunit MED25, and the AFP proteins AFP1, AFP2, AFP3 and AFP4. Interacts with TAP46. Interacts with the 36 kDa catalytic subunit (subunit C) of PP2A (PubMed:11489176, PubMed:12569131, PubMed:16247556, PubMed:17194765, PubMed:18484180, PubMed:22822206, PubMed:24357600).
Interacts with FYPP1 and FYPP3 (PubMed:22715043).
Interacts with FREE1 (via C-terminus) (PubMed:30962512).
Interacts with FYPP1 and FYPP3 (PubMed:22715043).
Interacts with FREE1 (via C-terminus) (PubMed:30962512).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9SJN0 | IAA30 Q9M1R4 | 3 | EBI-1778690, EBI-3946710 | |
BINARY | Q9SJN0 | KEG Q9FY48 | 2 | EBI-1778690, EBI-1955729 | |
BINARY | Q9SJN0 | SRK2A P43291 | 3 | EBI-1778690, EBI-401164 | |
BINARY | Q9SJN0 | SUMO1 P55852 | 2 | EBI-1778690, EBI-15763381 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-18 | Basic and acidic residues | ||||
Sequence: MVTRETKLTSEREVESSM | ||||||
Region | 1-37 | Disordered | ||||
Sequence: MVTRETKLTSEREVESSMAQARHNGGGGGENHPFTSL | ||||||
Compositional bias | 83-113 | Polar residues | ||||
Sequence: NQQQAAAAAGSHSVPANHNGFNNNNNNGGEG | ||||||
Region | 83-151 | Disordered | ||||
Sequence: NQQQAAAAAGSHSVPANHNGFNNNNNNGGEGGVGVFSGGSRGNEDANNKRGIANESSLPRQGSLTLPAP | ||||||
Compositional bias | 123-145 | Polar residues | ||||
Sequence: RGNEDANNKRGIANESSLPRQGS | ||||||
Region | 164-201 | Disordered | ||||
Sequence: IHRGGGSGNGGDSNGRSSSSNGQNNAQNGGETAARQPT | ||||||
Compositional bias | 173-197 | Polar residues | ||||
Sequence: GGDSNGRSSSSNGQNNAQNGGETAA | ||||||
Region | 218-238 | Disordered | ||||
Sequence: REHPTNPKPNPNPNQNQNPSS | ||||||
Domain | 355-418 | bZIP | ||||
Sequence: VERRQRRMIKNRESAARSRARKQAYTVELEAELNQLKEENAQLKHALAELERKRKQQYFESLKS | ||||||
Region | 357-376 | Basic motif | ||||
Sequence: RRQRRMIKNRESAARSRARK | ||||||
Region | 383-404 | Leucine-zipper | ||||
Sequence: LEAELNQLKEENAQLKHALAEL | ||||||
Region | 414-442 | Disordered | ||||
Sequence: ESLKSRAQPKLPKSNGRLRTLMRNPSCPL |
Sequence similarities
Belongs to the bZIP family. ABI5 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length442
- Mass (Da)47,007
- Last updated2000-05-01 v1
- ChecksumF879F51B99A99140
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1P8B106 | A0A1P8B106_ARATH | ABI5 | 444 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-18 | Basic and acidic residues | ||||
Sequence: MVTRETKLTSEREVESSM | ||||||
Compositional bias | 83-113 | Polar residues | ||||
Sequence: NQQQAAAAAGSHSVPANHNGFNNNNNNGGEG | ||||||
Compositional bias | 123-145 | Polar residues | ||||
Sequence: RGNEDANNKRGIANESSLPRQGS | ||||||
Compositional bias | 173-197 | Polar residues | ||||
Sequence: GGDSNGRSSSSNGQNNAQNGGETAA |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF334206 EMBL· GenBank· DDBJ | AAK19599.1 EMBL· GenBank· DDBJ | mRNA | ||
AC006921 EMBL· GenBank· DDBJ | AAD21438.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC09226.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | ANM62587.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT026517 EMBL· GenBank· DDBJ | ABH04624.1 EMBL· GenBank· DDBJ | mRNA |